LOCUS       Exported                2853 bp ds-DNA     circular SYN 17-OCT-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Nov 27, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        11..226
                     /label=H1 Promoter
     protein_bind    210..227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    complement(243..509)
                     /label=Mos
     promoter        complement(530..771)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     rep_origin      complement(1042..1630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1801..2661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2662..2766)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag aacgctgacg tcatcaaccc gctccaagga atcgcgggcc cagtgtcact
       61 aggcgggaac acccagcgcg cgtgcgccct ggcaggaaga tggctgtgag ggacagggga
      121 gtggcgccct gcaatatttg catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa
      181 atgtctttgg atttgggaat cttataagtt ccctatcagt gatagaggga aaaagcggcc
      241 gctaacaaca tctagggaat acttgaggca cttccccaaa ctcagttgtt ctctgcagct
      301 gagaggctcc ggtgagcggg tggcaccgta gatgacttgg tgtagagtca cgttgccccc
      361 aaactccatg attatggtac ctaggctgtt ggagtcttcg ggcgtgcgcg tgctggcagc
      421 cacaacccga actatgttgt cgtggcgtag tcttgcaatg ttcagttcag cccagaaact
      481 ccgctgggat gcacgtaggt ccttggtgca agctttttcc cggtgtttcg tcctttccac
      541 aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc
      601 attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac
      661 gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca
      721 gccttgtatc gtatatgcaa atatgaaggg aatcatggga aataggccct caattgcttt
      781 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1441 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     1501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2701 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2761 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     2821 attaacctat aaaaataggc gtatcacgag gcc
//
