LOCUS       Exported                2673 bp ds-DNA     circular SYN 13-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   tomas
  TITLE     Direct Submission
  JOURNAL   Exported Nov 27, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(25..334)
                     /label=lin28A
     promoter        complement(351..591)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     rep_origin      complement(862..1450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1621..2481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2482..2586)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag aaaaaagcgg ccgcgtttga cacttgtttc gcttcccgtc tggcaaggga
       61 aatatcacac agccttctac aaaagaactt ccctagccca gccacttggc aaagcctcgg
      121 gttctagggt ggttcctccc cacaaaagcc ttcacttgct ttcagataga gtgtggtagg
      181 agttcctttg tctcatcctt tccttccctc caaggaaaga cagggtgaca ctgggaaccc
      241 cggcctgaga cacggcagtg caagctaccc acgcaaccca cccttacccc cactttctcc
      301 actctgcaga ttgatgcctc tttccttaaa ctccgcggcc gcggtgtttc gtcctttcca
      361 caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc
      421 cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca
      481 cgtattttgt actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac
      541 agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct caattgcttt
      601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      661 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1261 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     1321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1921 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1981 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2041 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2101 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2161 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2221 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2281 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2341 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2401 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2461 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2581 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     2641 attaacctat aaaaataggc gtatcacgag gcc
//
