LOCUS       Exported                2720 bp ds-DNA     circular SYN 13-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   tomas
  TITLE     Direct Submission
  JOURNAL   Exported Nov 27, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    25..381
                     /label=Lin28A
     promoter        complement(398..638)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     rep_origin      complement(909..1497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1668..2528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2529..2633)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag aaaaaagcgg ccgcggagtt taaggaaaga ggcatcaatc tgcagagtgg
       61 agaaagtggg ggtaagggtg ggttgcgtgg gtagcttgca ctgccgtgtc tcaggccggg
      121 gttcccagtg tcaccctgtc tttccttgga gggaaggaaa ggatgagaca aaggaactcc
      181 taccacactc tatctgaaag caagtgaagg cttttgtggg gaggaaccac cctagaaccc
      241 gaggctttgc caagtggctg ggctagggaa gttcttttgt agaaggctgt gtgatatttc
      301 ccttgccaga cgggaagcga aacaagtgtc aaaccgcggc cgctaatctt gctgaaaaac
      361 tcgagccatc cggaagatct ggcggccgcg gtgtttcgtc ctttccacaa gatatataaa
      421 gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat
      481 aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact
      541 aatatctttg tgtttacagt caaattaatt ccaattatct ctctaacagc cttgtatcgt
      601 atatgcaaat atgaaggaat catgggaaat aggccctcaa ttgctttcca gtcgggaaac
      661 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      721 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1321 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1801 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2641 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2701 aataggcgta tcacgaggcc
//
