LOCUS       pRRL-EF1a-ERT2-E       11763 bp    DNA     circular     28-SEP-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|710275624|
COMMENT     VNTDBDATE|710275624|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRRL-EF1a-ERT2-ERT2-sox2TALE-VP64.gb|
COMMENT     VNTAUTHORNAME|zhaochen|
COMMENT     VNTOAUTHORNAME|McMahon Lab|
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FEATURES             Location/Qualifiers
     misc_feature    5672..6247
                     /vntifkey="21"
                     /label=WPRE
     promoter        4884..5206
                     /vntifkey="29"
                     /label=SV40\Promoter
     CDS             5207..5584
                     /vntifkey="4"
                     /label=Zeo
     promoter        23..1198
                     /vntifkey="29"
                     /label=EF-1a
     misc_feature    1358..2077
                     /vntifkey="21"
                     /label=N\terminal
     misc_feature    2078..3301
                     /vntifkey="21"
                     /label=12\Repeat
     misc_feature    3302..3361
                     /vntifkey="21"
                     /label=0.5\Repeat-NI
     misc_feature    3362..3898
                     /vntifkey="21"
                     /label=C\terminal
     primer          4735..4854
                     /vntifkey="27"
                     /label=SV40\PA\terminator
     misc_feature    3995..4009
                     /vntifkey="21"
                     /label=GSGSG
     misc_feature    3938..3994
                     /vntifkey="21"
                     /label=GCN4\peptide
     CDS             3938..4657
                     /vntifkey="4"
                     /label=GCN4\peptide_10*_v4
     misc_feature    1250..1321
                     /vntifkey="21"
                     /label=3xNLS
BASE COUNT     2918 a      2924 c      3284 g      2637 t 
ORIGIN
        1 ctagcccggg ctcgaccata tgataagctt tgcaaagatg gataaagttt taaacagaga 
       61 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      121 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      181 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      241 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      301 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      361 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      421 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      481 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      541 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      601 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      661 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      721 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
      781 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
      841 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
      901 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
      961 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1021 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1081 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1141 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctcctttc 
     1201 tagagccacc atgggtggcg cgcctccaaa aaagaagaga aaggtagatc caaaaaagaa 
     1261 gagaaaggta gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtagg 
     1321 attaattaat ggaagtggaa gtggaagtgg aggtaccatg tcgcggaccc ggctcccttc 
     1381 cccacccgca cccagcccag cgttttcggc cgactcgttc tcagacctgc ttaggcagtt 
     1441 cgacccctca ctgtttaaca catcgttgtt cgactccctt cctccgtttg gggcgcacca 
     1501 tacggaggcg gccaccgggg agtgggatga ggtgcagtcg ggattgagag ctgcggatgc 
     1561 accaccccca accatgcggg tggccgtcac cgctgcccga ccgccgaggg cgaagcccgc 
     1621 accaaggcgg agggcagcgc aaccgtccga cgcaagcccc gcagcgcaag tagatttgag 
     1681 aactttggga tattcacagc agcagcagga aaagatcaag cccaaagtga ggtcgacagt 
     1741 cgcgcagcat cacgaagcgc tggtgggtca tgggtttaca catgcccaca tcgtagcctt 
     1801 gtcgcagcac cctgcagccc ttggcacggt cgccgtcaag taccaggaca tgattgcggc 
     1861 gttgccggaa gccacacatg aggcgatcgt cggtgtgggg aaacagtgga gcggagcccg 
     1921 agcgcttgag gccctgttga cggtcgcggg agagctgaga gggcctcccc ttcagctgga 
     1981 cacgggccag ttgctgaaga tcgcgaagcg gggaggagtc acggcggtcg aggcggtgca 
     2041 cgcgtggcgc aatgcgctca cgggagcacc cctcaacctg accccagagc aggtcgtggc 
     2101 cattgcctcg aatggagggg gcaaacaggc gttggaaacc gtacaacgat tgctgccggt 
     2161 gctgtgccaa gcgcacggac tcaccccaga gcaggtcgtg gccattgcct cgaatggagg 
     2221 gggcaaacag gcgttggaaa ccgtacaacg attgctgccg gtgctgtgcc aagcgcacgg 
     2281 cctcacccca gagcaggtcg tggcaatcgc ctccaacatt ggcgggaaac aggcactcga 
     2341 gactgtccag cgcctgcttc ccgtgctgtg ccaagcgcac ggattaaccc cagagcaggt 
     2401 cgtggccatt gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac aacgattgct 
     2461 gccggtgctg tgccaagcgc acggcttaac cccagagcag gtcgtggcca ttgcctcgaa 
     2521 tggagggggc aaacaggcgt tggaaaccgt acaacgattg ctgccggtgc tgtgccaagc 
     2581 gcacggactt accccagagc aggtcgtggc gatcgcaagc cacgacggag gaaagcaagc 
     2641 cttggaaaca gtacagaggc tgttgcctgt gctgtgccaa gcgcacggcc ttaccccaga 
     2701 gcaggtcgtg gcgatcgcaa gccacgacgg aggaaagcaa gccttggaaa cagtacagag 
     2761 gctgttgcct gtgctgtgcc aagcgcacgg actaacccca gagcaggtcg tggcgatcgc 
     2821 aagccacgac ggaggaaagc aagccttgga aacagtacag aggctgttgc ctgtgctgtg 
     2881 ccaagcgcac gggctcaccc cagagcaggt cgtggccatt gcctcgaatg gagggggcaa 
     2941 acaggcgttg gaaaccgtac aacgattgct gccggtgctg tgccaagcgc acgggctaac 
     3001 cccagagcag gtcgtggcaa tcgcgagcaa taacggcgga aaacaggctt tggaaacggt 
     3061 gcagaggctc cttccagtgc tgtgccaagc gcacggccta accccagagc aggtcgtggc 
     3121 aatcgcctcc aacattggcg ggaaacaggc actcgagact gtccagcgcc tgcttcccgt 
     3181 gctgtgccaa gcgcacgggt taaccccaga gcaggtcgtg gcgatcgcaa gccacgacgg 
     3241 aggaaagcaa gccttggaaa cagtacagag gctgttgcct gtgctgtgcc aagcgcacgg 
     3301 actcacgcct gagcaggtag tggctattgc atccaatatc gggggcagac ccgcactgga 
     3361 gtcaatcgtg gcccagcttt cgaggccgga ccccgcgctg gccgcactca ctaatgatca 
     3421 tcttgtagcg ctggcctgcc tcggcggacg acccgccttg gatgcggtga agaaggggct 
     3481 cccgcacgcg cctgcattga ttaagcggac caacagaagg attcccgaga ggacatcaca 
     3541 tcgagtggca gatcacgcgc aagtggtccg cgtgctcgga ttcttccagt gtcactccca 
     3601 ccccgcacaa gcgttcgatg acgccatgac tcaatttggt atgtcgagac acggactgct 
     3661 gcagctcttt cgtagagtcg gtgtcacaga actcgaggcc cgctcgggca cactgcctcc 
     3721 cgcctcccag cggtgggaca ggattctcca agcgagcggt atgaaacgcg cgaagccttc 
     3781 acctacgtca actcagacac ctgaccaggc gagccttcat gcgttcgcag actcgctgga 
     3841 gagggatttg gacgcgccct cgcccatgca tgaaggggac caaactcgcg cgtcagctgg 
     3901 atccgccatc atacacgcgg ccgcaacaag cggtccggaa gaacttttga gcaagaatta 
     3961 tcatcttgag aacgaagtgg ctcgtcttaa gaaaggttct ggcagtggag aagaactgct 
     4021 ttcaaagaat taccacctgg aaaatgaggt agctagactg aaaaagggga gcggaagtgg 
     4081 ggaggagttg ctgagcaaaa attatcattt ggagaacgaa gtagcacgac taaagaaagg 
     4141 gtccggatcg ggtgaggagt tactctcgaa aaattatcat ctcgaaaacg aagtggctcg 
     4201 gctaaaaaag ggcagtggtt ctggagaaga gctattatct aaaaactacc acctcgaaaa 
     4261 tgaggtggca cgcttaaaaa agggaagtgg cagtggtgaa gagctactat ccaagaatta 
     4321 tcatcttgag aacgaggtag cgcgtttgaa gaagggttcc ggctcaggag aggaactgct 
     4381 ctcgaagaac tatcatcttg aaaatgaggt cgctcgatta aaaaagggat cgggcagtgg 
     4441 tgaggaacta ctttcaaaga attaccacct cgaaaacgaa gtagctcgat taaagaaagg 
     4501 ttcagggtcg ggtgaagaat tactgagtaa aaattatcat ctggaaaatg aggtagcgag 
     4561 actaaaaaag gggagtggtt ctggcgagga attgctatcg aaaaattatc atcttgagaa 
     4621 cgaagttgct aggctcaaaa agggctcagg ctcaggctga gtttaaacat cgcctgcagg 
     4681 ggaagtggaa gtgatatcgg aagtggaagc actagtggaa gtttcgaagt cgacttgttt 
     4741 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     4801 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatgtcgac 
     4861 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 
     4921 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     4981 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     5041 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     5101 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     5161 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca tggccaagtt 
     5221 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac 
     5281 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga 
     5341 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc 
     5401 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac 
     5461 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg 
     5521 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga 
     5581 ctgaccgatc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag 
     5641 accccacctg taggtttggc aagctcgaca atcaacctct ggattacaaa atttgtgaaa 
     5701 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 
     5761 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 
     5821 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 
     5881 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 
     5941 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 
     6001 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 
     6061 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 
     6121 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 
     6181 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 
     6241 tttgggccgc ctccccgcct ggaattcgag ctcggaacct ttaagaccaa tgacttacaa 
     6301 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 
     6361 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 
     6421 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 
     6481 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 
     6541 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 
     6601 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 
     6661 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     6721 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     6781 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     6841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
     6901 tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc aattcgccct 
     6961 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa 
     7021 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 
     7081 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 
     7141 ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
     7201 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
     7261 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
     7321 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 
     7381 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
     7441 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
     7501 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
     7561 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccaggtg gcacttttcg 
     7621 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 
     7681 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 
     7741 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 
     7801 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 
     7861 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 
     7921 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 
     7981 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 
     8041 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 
     8101 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 
     8161 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 
     8221 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 
     8281 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 
     8341 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 
     8401 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 
     8461 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 
     8521 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 
     8581 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 
     8641 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 
     8701 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 
     8761 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
     8821 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 
     8881 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 
     8941 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
     9001 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 
     9061 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 
     9121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 
     9181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 
     9241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
     9301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 
     9361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 
     9421 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 
     9481 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 
     9541 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 
     9601 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 
     9661 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 
     9721 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat 
     9781 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata 
     9841 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa 
     9901 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg 
     9961 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt 
    10021 gtatttaagt gcctagctcg atacaataaa cgggtctctc tggttagacc agatctgagc 
    10081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 
    10141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 
    10201 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cctgaaagcg 
    10261 aaagggaaac cagagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga 
    10321 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag 
    10381 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa 
    10441 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc 
    10501 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga 
    10561 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta 
    10621 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag 
    10681 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg 
    10741 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata 
    10801 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag 
    10861 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg 
    10921 agcagcagga agcactatgg gcgcagcctc aatgacgctg acggtacagg ccagacaatt 
    10981 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca 
    11041 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga 
    11101 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg 
    11161 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa 
    11221 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc 
    11281 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga 
    11341 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt 
    11401 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat 
    11461 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc 
    11521 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag 
    11581 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg 
    11641 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac 
    11701 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct 
    11761 cga 
//
