LOCUS Exported 11958 bp ds-DNA circular SYN 27-NOV-2018 DEFINITION Mammalian expression/lentiviral FHH-Mtor_HEAT1-9 (AA 1-409). ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11958) AUTHORS Open Biosystems TITLE Direct Submission JOURNAL Exported Nov 27, 2018 from SnapGene 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11958 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" /note="color: #ffffff" LTR 1..634 /label=5' LTR /note="5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" misc_feature 2063..2180 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" /note="color: #ffe4c4" CDS complement(2245..2619) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /note="color: #ccffcc" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" promoter complement(2639..2686) /label=EM7 promoter /note="synthetic bacterial promoter" /note="color: #ffffff; direction: LEFT" enhancer 2791..3094 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 3095..3298 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" primer_bind 3197..3216 /label=pLEX-MCS-FWD /note="color: black; sequence: CACCAAAATCAACGGGACTT" CDS 3390..3413 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /note="color: #cc99b2" /translation="DYKDDDDK" CDS 3420..3446 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /note="color: #cc99b2" /translation="YPYDVPDYA" CDS 3456..3473 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /note="color: #cc99b2" /translation="HHHHHH" CDS 3480..4709 /codon_start=1 /label=MTOR_HEAT1to9 /note="color: #cc99ff" /translation="MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQ HYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRI GRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEG RRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQ REPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGER LREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQ GLMGFGTSPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRL AAFRPSAFTD" misc_feature 4749..5321 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /note="color: #a6acb3" primer_bind complement(4800..4823) /label=pLEX-MCS-REV /note="color: black; sequence: ATATAGACAAACGCACACCGGCCT" CDS 5324..5926 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 5933..6521 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" /note="color: #ffffff" LTR 6728..6961 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 7068..7292 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" /note="color: #a6acb3" rep_origin 7338..7766 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00" promoter 7780..8109 /label=SV40 promoter /note="SV40 enhancer and early promoter" /note="color: #ffffff; direction: RIGHT" rep_origin 7960..8095 /label=SV40 ori /note="SV40 origin of replication" /note="color: #ffff00" CDS 8158..9183 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /note="color: #ccffcc" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" polyA_signal 9313..9434 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(9885..10473) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(10644..11504) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 10644 .. 11435 / #ccffcc 2: 11436 .. 11504 / #ccffcc / signal sequence Cleavage site after base 11435" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(11505..11609) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" polyA_signal 11657..11791 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac 2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag 2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt 2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat 2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag 2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg 2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac 2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg 2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt 2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc 2701 cgaggttcta gacgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc 2761 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 2821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 2881 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 2941 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 3001 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 3061 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa 3121 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa 3181 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc 3241 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg 3301 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag 3361 acaccgactc tactagagga tctaccatgg attataaaga tgacgacgat aaagcaggtt 3421 acccatacga tgtgcctgac tatgctgcag gttcacatca tcaccaccat catggatcca 3481 tgcttggaac cggacctgcc gccgccacca ccgctgccac cacatctagc aatgtgagcg 3541 tcctgcagca gtttgccagt ggcctaaaga gccggaatga ggaaaccagg gccaaagccg 3601 ccaaggagct ccagcactat gtcaccatgg aactccgaga gatgagtcaa gaggagtcta 3661 ctcgcttcta tgaccaactg aaccatcaca tttttgaatt ggtttccagc tcagatgcca 3721 atgagaggaa aggtggcatc ttggccatag ctagcctcat aggagtggaa ggtgggaatg 3781 ccacccgaat tggcagattt gccaactatc ttcggaacct cctcccctcc aatgacccag 3841 ttgtcatgga aatggcatcc aaggccattg gccgtcttgc catggcaggg gacactttta 3901 ccgctgagta cgtggaattt gaggtgaagc gagccctgga atggctgggt gctgaccgca 3961 atgagggccg gagacatgca gctgtcctgg ttctccgtga gctggccatc agcgtcccta 4021 ccttcttctt ccagcaagtg caacccttct ttgacaacat ttttgtggcc gtgtgggacc 4081 ccaaacaggc catccgtgag ggagctgtag ccgcccttcg tgcctgtctg attctcacaa 4141 cccagcgtga gccgaaggag atgcagaagc ctcagtggta caggcacaca tttgaagaag 4201 cagagaaggg atttgatgag accttggcca aagagaaggg catgaatcgg gatgatcgga 4261 tccatggagc cttgttgatc cttaacgagc tggtccgaat cagcagcatg gagggagagc 4321 gtctgagaga agaaatggaa gaaatcacac agcagcagct ggtacacgac aagtactgca 4381 aagatctcat gggcttcgga acaaaacctc gtcacattac ccccttcacc agtttccagg 4441 ctgtacagcc ccagcagtca aatgccttgg tggggctgct ggggtacagc tctcaccaag 4501 gcctcatggg atttgggacc tcccccagtc cagctaagtc caccctggtg gagagccggt 4561 gttgcagaga cttgatggag gagaaatttg atcaggtgtg ccagtgggtg ctgaaatgca 4621 ggaatagcaa gaactcgctg atccaaatga caatccttaa tttgttgccc cgcttggctg 4681 cattccgacc ttctgccttc acagattaac tcgagtctag agggcccgac gcgtctggcc 4741 accggtcgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa 4801 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg 4861 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 4921 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 4981 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 5041 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 5101 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta 5161 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 5221 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg 5281 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggcca ccgagtacaa 5341 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc 5401 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg 5461 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 5521 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 5581 ggcggtgttc gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc 5641 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 5701 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 5761 ccccggagtg gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc 5821 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 5881 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtta acaatcaacc 5941 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 6001 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 6061 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 6121 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 6181 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 6241 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 6301 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 6361 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 6421 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 6481 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 6541 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 6601 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 6661 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 6721 aggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact 6781 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6841 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6901 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6961 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 7021 gagagtgaga ggccttgaca ttgtttaaac ccgctgatca gcctcgactg tgccttctag 7081 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 7141 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 7201 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 7261 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 7321 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 7381 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 7441 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 7501 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 7561 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 7621 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 7681 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 7741 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 7801 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 7861 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 7921 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 7981 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 8041 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 8101 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgatg 8161 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 8221 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 8281 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 8341 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 8401 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 8461 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg 8521 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 8581 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 8641 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 8701 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 8761 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 8821 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 8881 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg 8941 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 9001 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 9061 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 9121 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa 9181 tagcacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 9241 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 9301 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 9361 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 9421 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 9481 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 9541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 9601 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 9661 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 9721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 9781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 9841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 9901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 9961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 10021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 10081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 10141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 10201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 10261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 10321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 10381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 10441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 10501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 10561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 10621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 10681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 10741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 10801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 10861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 10921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 10981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 11041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 11101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 11161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 11221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 11281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 11341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 11401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 11461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 11521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 11581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 11641 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag 11701 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 11761 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca 11821 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact 11881 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg 11941 tactacaaac ttagtagt //