LOCUS       NEW_DESTINATION_VECTOR_ 5268 bp ds-DNA     circular     26-APR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1403..1427
                     /label="misc_feature"
     misc_feature    2004..2075
                     /label="E. coli his operon terminator misc_feature"
     misc_feature    1436..1564
                     /label="Geneious type: Terminator"
     stem_loop       2012..2046
                     /label="stem_loop"
     misc_feature    4117..4121
                     /label="EcoRI misc_feature"
     misc_feature    join(4452..5268,1..472)
                     /label="URA3"
     misc_feature    2003..2003
                     /label="misc_feature"
     misc_feature    1586..1992
                     /label="ZetaDown-Not"
     misc_feature    496..695
                     /label="Geneious type: promoter prokaryote"
     misc_feature    490..493
                     /label="Geneious type: ligation"
     CDS             722..1396
                     /label="mrfp1 CDS"
     misc_feature    490..1566
                     /label="Geneious type: insert"
     primer_bind     4000..4019
                     /label="primer_bind"
     misc_feature    4131..4447
                     /label="ZetaUp"
     misc_feature    4117..4120
                     /label="Geneious type: ligation"
     CDS             complement(3017..3877)
                     /label="ampR (rev) CDS"
     misc_feature    4122..4126
                     /label="Bbsi misc_feature"
     stem_loop       complement(4089..4108)
                     /label="stem_loop"
     misc_feature    4126..4127
                     /label="Geneious type: Editing History Deletion"
     misc_feature    1565..1566
                     /label="Geneious type: motif"
     misc_feature    2258..2258
                     /label="ORI misc_feature"
     misc_feature    4128..4128
                     /label="Geneious type: Editing History Insertion"
     misc_feature    3156..3156
                     /label="Geneious type: Editing History Replacement"
     misc_feature    490..495
                     /label="Geneious type: motif"
     misc_feature    complement(4076..4079)
                     /label="end of terminator misc_feature"
     CDS             complement(2243..2857)
                     /label="rep(pMB1) (rev) CDS"
     misc_feature    704..715
                     /label="Geneious type: RBS"
     primer_bind     3139..3170
                     /label="primer_bind"
     primer_bind     complement(3139..3170)
                     /label="primer_bind"
     misc_feature    1998..2002
                     /label="PstI misc_feature"
     misc_feature    1999..2002
                     /label="Geneious type: ligation"
     primer_bind     complement(2139..2158)
                     /label="primer_bind"
ORIGIN
        1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
       61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag
      121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
      181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc
      241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
      301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
      361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
      421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaACGGtGAG
      481 ACCGGATCCc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
      721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
      781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
      841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
      901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
      961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
     1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
     1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1561 tatataTGTA CAgcGGTCTC tGAGTcatgt gtaacactcg ctctggagag ttagtcatcc
     1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg
     1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg
     1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt
     1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt
     1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac
     1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt
     1981 GCGGCCGCTG CGtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct
     2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
     2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
     3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct
     3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct GCGGCCGCtg
     4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta
     4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt
     4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga
     4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag
     4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt
     4441 tgctagaAGG Tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
     4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
     4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
     4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
     4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
     4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
     4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
     4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
     4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
     4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
     5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
     5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
     5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
     5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa
//