LOCUS       Exported                6301 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Eric Schreiter
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    57..223
                     /label=ITR
     promoter        267..714
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             748..804
                     /codon_start=1
                     /label=NES
                     /note="NES"
                     /translation="MLQNELALKLAGLDINKTG"
     CDS             811..828
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             835..2061
                     /codon_start=1
                     /label=rsCaMPARI
                     /label=rsCaMPARI-46
                     /translation="MVSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFLYGNRVFVKYPDNIQDYFKQSFPKGYSWERSMTFEDGGICYA
                     RSDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALL
                     LEGNAHYRALSSRRKFNKTGHALRAIGRLSSGGSGGSGGSGGSDQLTEEQIAEFKEAFS
                     LFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKM
                     KDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQ
                     VNYEEFVVMMTAKCLFRTTYKAKEKGVKLPGVHYVDHTIEILSHDKDYNKVKIYEYAVA
                     HSGLPDNARR"
     misc_feature    1354..1416
                     /label=M13
     misc_feature    1453..1893
                     /label=CaM
     CDS             2071..2778
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     misc_feature    2794..3382
                     /label=WPRE
     misc_feature    3416..3647
                     /label=SV40 polyadenylation signal
     misc_feature    complement(3676..3842)
                     /label=ITR
     misc_feature    4501..5358
                     /label=Amp
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatctctgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg
      301 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt
      361 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg
      421 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc
      481 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc
      541 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg
      601 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg
      661 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat
      721 tcaagctgct agcaaggatc cgccaccatg ctgcagaacg agcttgctct taagttggct
      781 ggacttgata ttaacaagac tggaggttct catcatcacc accaccatgg atccatggtg
      841 agtgcgatta agccagacat gaagatcaaa ctgcgtatgg aaggcaacgt aaacgggcac
      901 cactttgtga tcgacggaga tggtacaggc aagccttatg agggaaaaca gaccatggat
      961 cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc
     1021 ctgtacggca acagggtatt cgtgaaatat ccagacaaca tacaagacta ttttaagcag
     1081 tcgtttccta aggggtattc gtgggaacga agcatgactt tcgaagacgg gggcatttgc
     1141 tatgccagaa gcgacataac aatggaaggg gacactttct ataataaagt tcgattttat
     1201 ggtaccaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc
     1261 tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg gtgatgtaga gatggctttg
     1321 ttgcttgaag gaaatgccca ttaccgagcg ttgtcaagcc gccgcaagtt caacaaaacc
     1381 ggccatgcct tgcgtgcaat tggccgcctg tccagcggag gctctggcgg ttccggcggt
     1441 tcaggtggat cagaccagct gaccgaggag caaattgcag aatttaagga ggcgttctct
     1501 ttgtttgaca aagatggcga cggtacaatt acaaccaagg aattgggcac agtaatgcgc
     1561 tcccttggac agaatcccac tgaagccgaa cttcaagaca tgatcaatga ggttgatgct
     1621 gacggggatg ggactatcga ctttcctgag tttcttacca tgatggctcg caaaatgaag
     1681 gatacagaca gtgaggagga aattcgtgag gcctttcgtg tgtttgataa ggacgggaac
     1741 ggttatatct cagcggcgga gctgcgtcac gttatgacca acttaggcga aaagctgact
     1801 gatgaagaag tcgatgagat gatccgcgag gcggacatcg atggtgatgg tcaagtcaat
     1861 tatgaagagt ttgtggtgat gatgaccgcg aagtgcctct tcagaactac ttacaaagct
     1921 aaggagaagg gtgtcaagtt accaggcgtg cactatgtgg accacaccat tgagatttta
     1981 agccatgaca aagattacaa caaggttaag atctatgagt atgctgttgc tcattctgga
     2041 ttgcctgaca atgccagacg aggaggtagc gtgtctaagg gcgaagagct gatcaaggaa
     2101 aatatgcgta tgaaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca
     2161 ggtgaaggag aaggcagacc gtacgaggga gtgcaaacca tgaggatcaa agtcatcgag
     2221 ggaggacccc tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt
     2281 acctttatca agtacccggc cgacatccct gatttcttta aacagtcctt tcctgagggt
     2341 tttacttggg aaagagttac gagatacgaa gatggtggag tcgtcaccgt cacgcaggac
     2401 accagccttg aggatggcga gctcgtctac aacgtcaagg tcagaggggt aaactttccc
     2461 tccaatggtc ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg
     2521 tatccagcag atggtggtct gagaggatac actgacatcg cactgaaagt tgatggtggt
     2581 ggccatctgc actgcaactt cgtgacaact tacaggtcaa aaaagaccgt cgggaacatc
     2641 aagatgcccg gtgtccatgc cgttgatcac cgcctggaaa ggatcgagga gagtgacaat
     2701 gaaacctacg tagtgcaaag agaagtggca gttgccaaat acagcaacct tggtggtggc
     2761 atggacgagc tgtacaagta aaagcttatc gataatcaac ctctggatta caaaatttgt
     2821 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2881 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2941 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3001 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3061 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3121 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3181 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3241 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3301 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3361 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gacccgggcg gccgcttcga
     3421 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3481 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3541 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     3601 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt
     3661 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
     3721 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3781 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3841 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3901 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3961 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4021 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4081 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4141 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4201 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4261 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4321 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4381 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4441 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4501 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4561 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4621 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4681 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4741 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4801 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4861 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4921 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4981 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5041 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5101 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5161 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5221 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5281 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5341 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5401 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5641 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6181 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6241 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6301 g
//
