LOCUS       Antares2-N1             5855 bp ds-DNA     circular     19-AUG-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1321..1827
                     /label="teLuc"
     primer          2734..2753
                     /label="EBV_rev_primer"
     primer          3629..3648
                     /label="SV40pro_F_primer"
     promoter        520..589
                     /label="CMV_promoter"
     regulatory      631..640
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
     regulatory      65..352
                     /label="CAG_enhancer"
     promoter        3400..3668
                     /label="SV40_promoter"
     gene            2511..2517
                     /label="EGFP"
     origin          3567..3644
                     /label="SV40_origin"
     misc_feature    1834..2510
                     /label="CyOFP1 (B)"
     gene            3754..4542
                     /label="NeoR/KanR"
     primer          519..539
                     /label="CMV_fwd_primer"
     terminator      4720..4989
                     /label="TK_PA_terminator"
     regulatory      complement(3388..3603)
                     /label="SV40_enhancer"
     origin          5137..5756
                     /label="pBR322_origin"
     misc_feature    2643..2764
                     /label="SV40 poly(A) signal"
     promoter        10..562
                     /label="CMV_immearly_promoter"
     promoter        3288..3316
                     /label="AmpR_promoter"
     misc_feature    638..1311
                     /label="CyOFP1 (A)"
     origin          complement(2903..3209)
                     /label="f1_origin"
     primer          complement(3382..3402)
                     /label="pBABE_3_primer"
     misc_feature    631..2510
                     /label="Antares2"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttgcc gccaccatgg tgagcaaggg cgaggagctg
      661 atcaaggaga acatgagaag caagctgtac ctggaaggca gcgtgaacgg ccaccagttc
      721 aagtgcaccc acgaagggga gggcaagccc tacgagggca agcagaccaa caggatcaag
      781 gtggtggagg gaggccccct gccgttcgca ttcgacatcc tggccaccca ctttatgtac
      841 gggagcaagg tgttcatcaa gtaccccgcc gacctccccg attattttaa gcagtccttc
      901 cctgagggct tcacatggga gagagtcatg gtgttcgaag acgggggcgt gctgaccgcc
      961 acccaggaca ccagcctcca ggacggcgag ctcatctaca acgtcaaggt cagaggggtg
     1021 aacttcccag ccaacggccc cgtgatgcag aagaaaacac tgggctggga gcccagcacc
     1081 gagaccatgt accccgctga cggcggcctg gaaggcagat gcgacaaggc cctgaagctc
     1141 gtgggcgggg gccacctgca cgtcaacttc aagaccacat acaagtccaa gaaacccgtg
     1201 aagatgcccg gcgtccacta cgtggaccgc agactggaaa gaatcaagga ggccgacaac
     1261 gagacctacg tcgagcagta cgagcacgct gtggccagat actccaacct gggcggaggc
     1321 ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa cttgagtcaa
     1381 gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg
     1441 atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc
     1501 ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg
     1561 taccctgtgg ataatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac
     1621 ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc
     1681 gacggcaaaa agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag
     1741 cgcctgatca accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc
     1801 tggcgtctgc atgaacgcat tctggcgagg cacgagctga tcaaggagaa catgagaagc
     1861 aagctgtacc tggaaggcag cgtgaacggc caccagttca agtgcaccca cgaaggggag
     1921 ggcaagccct acgagggcaa gcagaccaac aggatcaagg tggtggaggg aggccccctg
     1981 ccgttcgcat tcgacatcct ggccacccac tttatgtacg ggagcaaggt gttcatcaag
     2041 taccccgccg acctccccga ttattttaag cagtccttcc ctgagggctt cacatgggag
     2101 agagtcatgg tgttcgaaga cgggggcgtg ctgaccgcca cccaggacac cagcctccag
     2161 gacggcgagc tcatctacaa cgtcaaggtc agaggggtga acttcccagc caacggcccc
     2221 gtgatgcaga agaaaacact gggctgggag cccagcaccg agaccatgta ccccgctgac
     2281 ggcggcctgg aaggcagatg cgacaaggcc ctgaagctcg tgggcggggg ccacctgcac
     2341 gtcaacttca agaccacata caagtccaag aaacccgtga agatgcccgg cgtccactac
     2401 gtggaccgca gactggaaag aatcaaggag gccgacaacg agacctacgt cgagcagtac
     2461 gagcacgctg tggccagata ctccaacctg ggcggaggca tggacgagct gtacaagtaa
     2521 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2581 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2641 ttAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA
     2701 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT
     2761 CTTAaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     2821 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     2881 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     2941 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3001 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3061 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3121 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3181 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     3241 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3301 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     3361 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3421 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3481 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3541 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3601 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3661 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     3721 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     3781 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     3841 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     3901 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     3961 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4021 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4081 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4141 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4201 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4261 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4321 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4381 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     4441 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     4501 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     4561 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     4621 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     4681 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     4741 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     4801 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     4861 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     4921 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     4981 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5041 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5101 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5161 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5221 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5281 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5341 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5401 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5461 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5521 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5581 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5641 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5701 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5761 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5821 ctgattctgt ggataaccgt attaccgcca tgcat
//