LOCUS       PB-fLuc-tdTomato_(plann 8496 bp ds-DNA     circular     25-AUG-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          6483..6502
                     /label="EBV_rev_primer"
     primer          complement(6031..6053)
                     /label="M13_pUC_rev_primer"
     origin          complement(6750..7369)
                     /label="pBR322_origin"
     CDS             3631..5061
                     /label="tdTomato"
     promoter        complement(6067..6096)
                     /label="lac_promoter"
     origin          6237..6314
                     /label="SV40_origin"
     promoter        complement(6014..6032)
                     /label="M13_reverse_primer"
     primer          6299..6318
                     /label="SV40pro_F_primer"
     misc_feature    5625..6000
                     /label="5' Terminal Repeat"
     primer          1829..1848
                     /label="pCAG_F_primer"
     misc_feature    27..456
                     /label="3' Terminal Repeat"
     promoter        complement(8426..8454)
                     /label="AmpR_promoter"
     terminator      5105..5606
                     /label="rb_glob_PA_terminator"
     CDS             1933..3582
                     /label="luciferase"
     misc_feature    457..1870
                     /label="CAG Promoter"
     promoter        6202..6381
                     /label="SV40_promoter"
     misc_feature    5961..5995
                     /label="5' ITR specific sequence"
     feature         complement(390..423)
                     /label="loxP"
     gene            complement(7524..8384)
                     /label="Ampicillin"
     misc_feature    31..93
                     /label="3' ITR specific sequence"
     terminator      6395..6526
                     /label="SV40_PA_terminator"
ORIGIN
        1 CCCCGAAAAG TGCCACCTGG GTCGACTTAA CCCTAGAAAG ATAATCATAT TGTGACGTAC
       61 GTTAAAGATA ATCATGCGTA AAATTGACGC ATGTGTTTTA TCGATCTGTA TATCGAGGTT
      121 TATTTATTAA TTTGAATAGA TATTAAGTTT TATTATATTT ACACTTACAT ACTAATAATA
      181 AATTCAACAA ACAATTTATT TATGTTTATT TATTTATTAA AAAAAAACAA AAACTCAAAA
      241 TTTCTTCTAT AAAGTAACAA AACTTTTAAA CATTCTCTCT TTTACAAAAA TAAACTTATT
      301 TTGTACTTTA AAAACAGTCA TGTTGTATTA TAAAATAAGT AATTAGCTTA ACTTATACAT
      361 AATAGAAACA AATTATACTT ATTAATCGCA TAACTTCGTA TAATGTATGC TATACGAAGT
      421 TATGCGATTA AGGGATCTGT AGGGCGCAGT AGTCCAACTA GTTATTAATA GTAATCAATT
      481 ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTGCTATTAC CATGGGTCGA
      541 GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC CCCCAATTTT
      601 GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCGGGGGGGG GGGGGGCGCG
      661 CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC GGGGCGGGGC GAGGCGGAGA GGTGCGGCGG
      721 CAGCCAATCA GAGCGGCGCG CTCCGAAAGT TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC
      781 GGCCCTATAA AAAGCGAAGC GCGCGGCGGG CGGGAGTCGC TGCGTTGCCT TCGCCCCGTG
      841 CCCCGCTCCG CGCCGCCTCG CGCCGCCCGC CCCGGCTCTG ACTGACCGCG TTACTCCCAC
      901 AGGTGAGCGG GCGGGACGGC CCTTCTCCTC CGGGCTGTAA TTAGCGCTTG GTTTAATGAC
      961 GGCTCGTTTC TTTTCTGTGG CTGCGTGAAA GCCTTAAAGG GCTCCGGGAG GGCCCTTTGT
     1021 GCGGGGGGGA GCGGCTCGGG GGGTGCGTGC GTGTGTGTGT GCGTGGGGAG CGCCGCGTGC
     1081 GGCCCGCGCT GCCCGGCGGC TGTGAGCGCT GCGGGCGCGG CGCGGGGCTT TGTGCGCTCC
     1141 GCGTGTGCGC GAGGGGAGCG CGGCCGGGGG CGGTGCCCCG CGGTGCGGGG GGGCTGCGAG
     1201 GGGAACAAAG GCTGCGTGCG GGGTGTGTGC GTGGGGGGGT GAGCAGGGGG TGTGGGCGCG
     1261 GCGGTCGGGC TGTAACCCCC CCCTGCACCC CCCTCCCCGA GTTGCTGAGC ACGGCCCGGC
     1321 TTCGGGTGCG GGGCTCCGTG CGGGGCGTGG CGCGGGGCTC GCCGTGCCGG GCGGGGGGTG
     1381 GCGGCAGGTG GGGGTGCCGG GCGGGGCGGG GCCGCCTCGG GCCGGGGAGG GCTCGGGGGA
     1441 GGGGCGCGGC GGCCCCGGAG CGCCGGCGGC TGTCGAGGCG CGGCGAGCCG CAGCCATTGC
     1501 CTTTTATGGT AATCGTGCGA GAGGGCGCAG GGACTTCCTT TGTCCCAAAT CTGGCGGAGC
     1561 CGAAATCTGG GAGGCGCCGC CGCACCCCCT CTAGCGGGCG CGGGCGAAGC GGTGCGGCGC
     1621 CGGCAGGAAG GAAATGGGCG GGGAGGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT
     1681 CCATCTCCAG CCTCGGGGCT GCCGCAGGGG GACGGCTGCC TTCGGGGGGG ACGGGGCAGG
     1741 GCGGGGTTCG GCTTCTGGCG TGTGACCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT
     1801 GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT
     1861 TTTGGCAAAG AATTCTGCAG TCGACGgtac cgagctcgga tccactagtc cagtgtggtg
     1921 gaattcacca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca
     1981 ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg
     2041 cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac
     2101 ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat
     2161 cggatcgtgg tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg
     2221 ttcatcggtg tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac
     2281 agcatgggca tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc
     2341 ctcaacgtgc aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc
     2401 gactaccagg gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc
     2461 aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg
     2521 aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt
     2581 gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct
     2641 atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg
     2701 atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc
     2761 ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct
     2821 aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg
     2881 gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc
     2941 cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac
     3001 gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg
     3061 gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg
     3121 atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc
     3181 tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac
     3241 cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc
     3301 atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat
     3361 gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag
     3421 gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt
     3481 gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc
     3541 gagattctca ttaaggccaa gaagggcggc aagatcgccg tgggagatct aggaggtgga
     3601 ggtagcggtg gaggtggaag ccaggatccg atggtgagca agggcgagga ggtcatcaaa
     3661 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     3721 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     3781 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     3841 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     3901 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     3961 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4021 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4081 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4141 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4201 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4261 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc
     4321 ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc
     4381 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     4441 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     4501 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     4561 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     4621 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     4681 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     4741 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     4801 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     4861 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     4921 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     4981 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc
     5041 atggacgagc tgtacaagta agaattctgc agatatccag cacagtggcG GCCGCACTCC
     5101 TCAGGTGCAG GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCAATGCCC TGGCTCACAA
     5161 ATACCACTGA GATCTTTTTC CCTCTGCCAA AAATTATGGG GACATCATGA AGCCCCTTGA
     5221 GCATCTGACT TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT
     5281 TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTAAAAC ATCAGAATGA
     5341 GTATTTGGTT TAGAGTTTGG CAACATATGC CATATGCTGG CTGCCATGAA CAAAGGTGGC
     5401 TATAAAGAGG TCATCAGTAT ATGAAACAGC CCCCTGCTGT CCATTCCTTA TTCCATAGAA
     5461 AAGCCTTGAC TTGAGGTTAG ATTTTTTTTA TATTTTGTTT TGTGTTATTT TTTTCTTTAA
     5521 CATCCCTAAA ATTTTCCTTA CATGTTTTAC TAGCCAGATT TTTCCTCCTC TCCTGACTAC
     5581 TCCCAGTCAT AGCTGTCCCT CTTCTCTTAT GAAGATCCCT CGACCTGCAG GCTCCAGCCT
     5641 ACACAATCGC TCAAGACGTG TAATGCTTTT ATTATATATT AGTCACGATA TCTATAACAA
     5701 GAAAATATAT ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC
     5761 GTATGAGTTA AATCTTAAAA GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG
     5821 GTCGTTATAG TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT
     5881 CCAAGCGGCG ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG
     5941 AGAGCAATAT TTCAAGAATG CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAA
     6001 AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT
     6061 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC
     6121 TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC
     6181 CAGCGGATCC GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC
     6241 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA
     6301 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT
     6361 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TAACTTGTTT ATTGCAGCTT ATAATGGTTA
     6421 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
     6481 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCGCT GCATTAATGA
     6541 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
     6601 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
     6661 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
     6721 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
     6781 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
     6841 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
     6901 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA
     6961 TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
     7021 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
     7081 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
     7141 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
     7201 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
     7261 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
     7321 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
     7381 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     7441 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     7501 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     7561 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     7621 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG
     7681 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     7741 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     7801 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     7861 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     7921 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     7981 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     8041 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     8101 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     8161 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     8221 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     8281 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     8341 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     8401 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     8461 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTT
//