LOCUS       pACYC-Cas12c2-noncoding 5978 bp ds-DNA     circular     03-DEC-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             1082..2272
                     /label="tet"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(82..994)
                     /label="p15A_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2763..2792
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    751..857
                     /label="RNA I start"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gene            complement(5039..5698)
                     /label="CAT/CamR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2729..2756
                     /label="LacO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2711..2729
                     /label="pET28 T7 promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(5042..5698)
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(313..348)
                     /label="+1 Origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1085..2269
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(152..857)
                     /label="RNA Primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(4705..4735)
                     /label="LARF F10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1998..2028)
                     /label="F12 LARF substrate"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5427..5457
                     /label="F7 LARF substrate"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2661..2710
                     /label="l_flank"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2793..4443
                     /label="Cas12c2 noncoding"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4444..4493
                     /label="r_flank"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
       61 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact
      121 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc
      181 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
      241 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
      301 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
      361 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
      421 aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc
      481 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
      541 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
      601 ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac
      661 atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag
      721 tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc
      781 cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag
      841 gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat
      901 catcttatta atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta
      961 gcacctgaag tcagccccat acgatataag ttgtaattct catgtttgac agcttatcat
     1021 cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
     1081 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
     1141 cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag
     1201 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
     1261 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
     1321 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta
     1381 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     1441 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     1501 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     1561 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     1621 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
     1681 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     1741 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
     1801 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
     1861 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
     1921 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
     1981 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
     2041 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
     2101 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc
     2161 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
     2221 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc
     2281 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg
     2341 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc
     2401 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc
     2461 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
     2521 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
     2581 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc
     2641 cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata
     2701 ctatgactga TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA
     2761 GATTTACACT TTATGCTTCC GGCTCGTATG TTTCTAGACT CGAGATGCTG GCCAAGGAAT
     2821 TTGGTTTGTC GGATGTAATG ATTGGGAAGC GATGCAAAAA ACTGGGTGTT GTAAAACCAA
     2881 AGCCGGGATT CTGGGCGAAG GTGGACGCTG GGAAAGTGCC ACATCCCCAA GGAAAGAAAG
     2941 TAGCCGGGCA GTAAATAACG CTTTGGAATA TGAGACGTAT CTGTAGAATT TTTGGTATCC
     3001 AGTAAAAAGA GCAAAACGAA ATGACAAAAC ACTCAATACC CCTTCACGCA TTCCGCAACT
     3061 CCGGCGCAGA CGCCAGAAAA TGGAAAGGCC GCATTGCTTT GCTGGCCAAG CGCGGCAAAG
     3121 AAACGATGCG AACACTGCAG TTTCCGCTCG AAATGAGCGA GCCTGAAGCA CTGCGCCGTG
     3181 CGCCCAAGAA TCGCCGCACC AAGGACACCA CCGTAAGCGA GTATCACTGT GTCTTTTCAG
     3241 ACTGCGGAAA GACAATGCAC GCGGATGAGA ATGCGGCAGT GAATATCGGC GGAAAGTTCA
     3301 TTGCAGATAT CGAAAAATAG TTTGCACAAA GTGCGAGAAA AGATATAATA CACACATACC
     3361 ACCCGTGCAT TTCTGGATCA ATGATCCGTA CCTCAATGTC CGGGCGCGCA GCTAGAGCGA
     3421 CCTGAAATCT GCACGAAAAC CGGCGAAAGC CGGTTTTTTG TTGCCTGTAG TTTGAGTTTA
     3481 TTTCTTTGCG CCTGGGATGG CAAAGCCAGC TTTCACCGGG CGACCGCCGA TACGCTCACC
     3541 TTCGACTGGG ATCACAAGCC TTGCCGTTTC CTTGGCGAGA TAGCTGCGCA GTGCCAACAC
     3601 TTCCGCATGC AAGACATTTT TACGCCTACC ACCTTTGTAC ACGAACTTAT AGCGAGCTTT
     3661 GGATTTTTGG ATAGGTTCAA TCGAATTTAG GTAGGGTATC CAAAGTGCCG CTCGACCACC
     3721 ACGGATGTTT TTCCATGTCC AAATAGAGTC TGGATGTTGA AATTGTGAGA TTTCCATATG
     3781 CAAGCTGGGT GGCTGTTAAA AATGAATTTC ATGATAATAC ATAATGTTAT ATTATGAACG
     3841 ACTTCCCCCG CGTCACTAGC AGGATTCAGG TTGGGTTTGA GGGGTCAGCG TACTCAGGAA
     3901 GCAGGATTCA GGTTGGGTTT GAGGAGCTGG ATGCCGTTCC ACAGCAGGAT TCAGGTTGGG
     3961 TTTGAGGGGC TGGCCAGAAC AAGAAAGCAG GATTCAGGTT GGGTTTGAGG CTGTCTCCTT
     4021 TTGGAAAAGC AGGATTCAGG TTGGGTTTGA GGGCTCAATC CTATTCAGAA AGCAGGATTC
     4081 AGGTTGGGTT TGAGGTGGCC AGGAGAACAG AAAGCAGGAT TCAGGTTGGG TTTGAGGTGT
     4141 GCATGAGGAT GCTAAAGCAG GATTCAGGTT GGGTTTGAGG CTAGCGGGAT TACAACAAAG
     4201 CAGGATTTAG GTTGGATTTG AGGGTGCCAT CAAAATTTGG TAGCAGGATT CAGGTTGGGG
     4261 TTGAGGCCGA AAAGATTCAG ATGAGCAGGA TTCAGGTTGG GTTTGAGGCT CGAGCTGCTA
     4321 ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC
     4381 CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG
     4441 GATgagtcaa cgccatgagc ggcctcattt cttattctga gttacaacag tccgcaccgc
     4501 tgtccggtag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     4561 tcttctttat catgcaactc gtaggacagg tgccggcagc gcccaacagt cccccggcca
     4621 cggggcctgc caccataccc acgccgaaac aagcgccctg caccattatg ttccggatct
     4681 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
     4741 aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa ttattacctc
     4801 cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct
     4861 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
     4921 ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta
     4981 tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt
     5041 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     5101 ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     5161 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     5221 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     5281 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     5341 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     5401 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     5461 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa
     5521 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     5581 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     5641 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     5701 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     5761 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa
     5821 agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt
     5881 gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac
     5941 tgatttagtg tatgatggtg tttttgaggt gctccagt
//