LOCUS       pACYC-OspCas12c-noncodi 5494 bp ds-DNA     circular     03-DEC-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1998..2028)
                     /label="F12 LARF substrate"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(82..994)
                     /label="p15A_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(4558..5214)
                     /label="CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1082..2272
                     /label="tet"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4943..4973
                     /label="F7 LARF substrate"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2793..3959
                     /label="OspCas12c noncoding"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2763..2792
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2661..2710
                     /label="l_flank"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(152..857)
                     /label="RNA Primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(313..348)
                     /label="+1 Origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1085..2269
                     /label="TetR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2711..2729
                     /label="pET28 T7 promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            complement(4555..5214)
                     /label="CAT/CamR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2729..2756
                     /label="LacO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3960..4009
                     /label="r_flank"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4221..4251)
                     /label="LARF F10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    751..857
                     /label="RNA I start"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
       61 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact
      121 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc
      181 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
      241 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
      301 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
      361 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
      421 aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc
      481 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
      541 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
      601 ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac
      661 atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag
      721 tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc
      781 cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag
      841 gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat
      901 catcttatta atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta
      961 gcacctgaag tcagccccat acgatataag ttgtaattct catgtttgac agcttatcat
     1021 cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
     1081 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
     1141 cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag
     1201 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
     1261 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
     1321 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta
     1381 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     1441 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     1501 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     1561 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     1621 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
     1681 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     1741 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
     1801 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
     1861 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
     1921 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
     1981 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
     2041 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
     2101 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc
     2161 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
     2221 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc
     2281 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg
     2341 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc
     2401 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc
     2461 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
     2521 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
     2581 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc
     2641 cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata
     2701 ctatgactga TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA
     2761 GATTTACACT TTATGCTTCC GGCTCGTATG TTTCTAGACT CGAGATGCGC ATACAAAACC
     2821 CATTGCAATG GTAGTTATAT AGTGATACAA GTATTGGATA TGAATGACGA AAATAAAACA
     2881 AAAAGCCACC CAAATTCAAT TTGGTATTGG TGTTCTGGTG AGAAACTATG GTATTTGCCT
     2941 TACTCTGGGA CACCTAAAAG AACATCTAAA TCTGTTTGGA CGGTTTCTGG TTCTTTTGGC
     3001 CAACTAGATA CATTAATCCA TGGGCAGGTA ATTGCTCTGC GACATTATTT AAATGGAAAT
     3061 ATGCAGCGTT ATTTACCCCC CGTTGAAGAA CTAGCAAAAG AGCGGGGGCG TAAGCGAGCA
     3121 GTCAGCTATA CACTACCTGG CCAAATTTGG AAAAAATAAT CATGACAAAG TTAAGACACC
     3181 GACAGAAAAA ATTAACACAC GACTGGGCTG GCTCCAAAAA GAGGGAAGTA TTAGGCTCAA
     3241 ATGGCAAGTT TCAGAATCCG TTGTTAATGC CGGTTAAAAA AGGTCAGGTT ACTGAGTTCC
     3301 GGAAAGCGTT TCATTCACCT GAGTTTATCG CTAAAAAACG CGGCATAGGG TCTGAGTATT
     3361 TTTGCGCTTA TTCCGATTGC AACTCATCCT TAAATTCTGA AGCTAACGCA GCTGCTAACG
     3421 TAGCGCAAAA ATTTCAAAAA CAGTTATTTT TTGAGTTATA AAATGCTTAA TAAAAATCGC
     3481 TTAAAATTAC TTGTTTTCTG GTTCTAATTA GGTAGTATAA AGGTTCATCG TTGGTATGGC
     3541 TGTGTTCTTC TGAACGCACA TCATTCTCTT AGAGACGTCT ACATACCAAC CCACGATGGG
     3601 GAGCGTGGAC AACAAAATCT CCCCACTACT TTCTTTTTTA GACTTAACGT TTGTTGTTTG
     3661 TTATTTTTCT ACTCTTAATT TATATCTTCT ATTTTTGTTG CCTGTGCTTT CATTCCCCCC
     3721 GCTTCCATAG TTAAACCTCT GTTTATTATC GTACTTATTG CTACAGCAGC CTGTAATGCG
     3781 TTTTCCATGT GTTGTTTTGC ATTCACTGGT TCTGCATTTC CGCACTCGAG CTGCTAACAA
     3841 AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT
     3901 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATg
     3961 agtcaacgcc atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgtc
     4021 cggtagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     4081 ctttatcatg caactcgtag gacaggtgcc ggcagcgccc aacagtcccc cggccacggg
     4141 gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc ggatctgcat
     4201 cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctaacc
     4261 gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg
     4321 gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg
     4381 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
     4441 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
     4501 ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
     4561 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     4621 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     4681 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     4741 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     4801 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     4861 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     4921 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     4981 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     5041 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     5101 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     5161 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     5221 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     5281 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt
     5341 ggcccagggc ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc
     5401 ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat
     5461 ttagtgtatg atggtgtttt tgaggtgctc cagt
//