LOCUS       pET28a-mH6-Cas12g1      7802 bp ds-DNA     circular     20-NOV-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS             2591..2627
                     /label="pET RBS + 3' UTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      6736..7418
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(5878..6690)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2515..2542
                     /label="LacO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2497..2515
                     /label="pET28 T7 promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      5344..5650
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2549..2578
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      5327..5782
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2628..2669
                     /label="mH6 tag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2670..4976
                     /label="Cas12g1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
       61 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
      121 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
      181 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
      241 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
      301 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
      361 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
      421 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
      481 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
      541 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
      601 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
      661 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
      721 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
      781 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
      841 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
      901 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
      961 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     1021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     1141 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     1201 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     1261 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     1321 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     1381 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     1441 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     1501 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     1561 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     1621 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     1681 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     1741 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     1801 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     1861 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     1921 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     1981 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     2041 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     2101 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     2161 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     2221 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     2281 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     2341 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     2401 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     2461 ccggcgtaga ggatcgagat ctcgatcccg cgaaatTAAT ACGACTCACT ATAGGGGAAT
     2521 TGTGAGCGGA TAACAATTCC CCTCTAGATT TACACTTTAT GCTTCCGGCT CGTATGTTTC
     2581 TAGAAAGCTT AAATAATTTT GTTTAACTTT AAGAAGGAGA TTTAAATatg AAAATCGAAG
     2641 AAGGTAAAGG TCACCATCAC CATCACCACA TGGCGCAGGC GAGCAGCACC CCGGCGGTGA
     2701 GCCCGCGTCC GCGTCCGCGT TACCGTGAGG AACGTACCCT GGTTCGTAAA CTGCTGCCGC
     2761 GTCCGGGTCA AAGCAAGCAG GAGTTCCGTG AAAACGTGAA GAAACTGCGT AAGGCGTTCC
     2821 TGCAGTTTAA CGCGGATGTG AGCGGTGTTT GCCAATGGGC GATCCAGTTC CGTCCGCGTT
     2881 ATGGCAAACC GGCGGAGCCG ACCGAAACCT TTTGGAAGTT CTTTCTGGAG CCGGAAACCA
     2941 GCCTGCCGCC GAACGACAGC CGTAGCCCGG AGTTCCGTCG TCTGCAGGCG TTTGAAGCGG
     3001 CGGCGGGTAT TAACGGTGCG GCGGCGCTGG ACGATCCGGC GTTCACCAAC GAGCTGCGTG
     3061 ATAGCATTCT GGCGGTGGCG AGCCGTCCGA AGACCAAAGA AGCGCAACGT CTGTTTAGCC
     3121 GTCTGAAAGA CTACCAGCCG GCGCACCGTA TGATCCTGGC GAAGGTTGCG GCGGAGTGGA
     3181 TTGAAAGCCG TTACCGTCGT GCGCATCAGA ACTGGGAGCG TAACTATGAG GAATGGAAGA
     3241 AAGAAAAGCA AGAGTGGGAA CAGAACCACC CGGAGCTGAC CCCGGAGATC CGTGAAGCGT
     3301 TCAACCAAAT TTTTCAGCAA CTGGAAGTGA AGGAAAAACG TGTTCGTATC TGCCCGGCGG
     3361 CGCGTCTGCT GCAAAACAAA GACAACTGCC AGTATGCGGG CAAGAACAAA CACAGCGTGC
     3421 TGTGCAACCA ATTCAACGAG TTTAAGAAAA ACCATCTGCA GGGCAAGGCG ATCAAGTTCT
     3481 TTTACAAGGA TGCGGAAAAG TATCTGCGTT GCGGTCTGCA AAGCCTGAAA CCGAACGTTC
     3541 AGGGCCCGTT CCGTGAGGAC TGGAACAAGT ACCTGCGTTA TATGAACCTG AAAGAGGAAA
     3601 CCCTGCGTGG CAAGAACGGT GGCCGTCTGC CGCACTGCAA AAACCTGGGC CAAGAGTGCG
     3661 AATTTAACCC GCATACCGCG CTGTGCAAGC AGTACCAGCA ACAGCTGAGC AGCCGTCCGG
     3721 ACCTGGTGCA ACACGATGAA CTGTACCGTA AATGGCGTCG TGAGTATTGG CGTGAACCGC
     3781 GTAAGCCGGT GTTCCGTTAC CCGAGCGTTA AACGTCATAG CATCGCGAAG ATTTTTGGCG
     3841 AGAACTATTT CCAGGCGGAC TTTAAGAACA GCGTGGTTGG TCTGCGTCTG GATAGCATGC
     3901 CGGCGGGCCA GTACCTGGAG TTCGCGTTTG CGCCGTGGCC GCGTAACTAT CGTCCGCAAC
     3961 CGGGCGAAAC CGAAATCAGC AGCGTGCACC TGCATTTCGT TGGCACCCGT CCGCGTATTG
     4021 GCTTCCGTTT TCGTGTTCCG CACAAACGTA GCCGTTTTGA CTGCACCCAG GAAGAGCTGG
     4081 ATGAGCTGCG TAGCCGTACC TTCCCGCGTA AAGCGCAAGA TCAGAAGTTT CTGGAGGCGG
     4141 CGCGTAAGCG TCTGCTGGAA ACCTTCCCGG GTAACGCGGA GCAGGAACTG CGTCTGCTGG
     4201 CGGTGGACCT GGGCACCGAT AGCGCGCGTG CGGCGTTCTT TATCGGCAAA ACCTTCCAAC
     4261 AGGCGTTTCC GCTGAAGATC GTTAAAATTG AGAAGCTGTA CGAACAGTGG CCGAACCAAA
     4321 AACAAGCGGG CGACCGTCGT GATGCGAGCA GCAAGCAACC GCGTCCGGGT CTGAGCCGTG
     4381 ACCACGTGGG CCGTCATCTG CAAAAAATGC GTGCGCAGGC GAGCGAGATT GCGCAAAAGC
     4441 GTCAAGAACT GACCGGCACC CCGGCGCCGG AAACCACCAC CGACCAAGCG GCGAAGAAAG
     4501 CGACCCTGCA GCCGTTCGAT CTGCGTGGCC TGACCGTTCA CACCGCGCGT ATGATCCGTG
     4561 ACTGGGCGCG TCTGAACGCG CGTCAAATCA TTCAGCTGGC GGAGGAAAAC CAGGTGGATC
     4621 TGATTGTTCT GGAAAGCCTG CGTGGTTTCC GTCCGCCGGG TTACGAGAAC CTGGACCAAG
     4681 AAAAGAAACG TCGTGTGGCG TTCTTTGCGC ATGGTCGTAT CCGTCGTAAA GTGACCGAGA
     4741 AGGCGGTTGA ACGTGGCATG CGTGTGGTTA CCGTGCCGTA TCTGGCGAGC AGCAAAGTTT
     4801 GCGCGGAGTG CCGTAAGAAA CAAAAAGATA ACAAGCAGTG GGAGAAGAAC AAGAAACGTG
     4861 GTCTGTTCAA GTGCGAGGGT TGCGGCAGCC AAGCGCAGGT TGACGAAAAC GCGGCGCGTG
     4921 TGCTGGGTCG TGTGTTCTGG GGTGAGATCG AACTGCCGAC CGCGATTCCG TAATAAAAGC
     4981 TTCGACCCTT GACGGAACTG GCCGATTTGC TAAAATATTT TTGATGCTTA CTTAGTCATC
     5041 TGGTTGGCAA ACCTCCGCGG ACCTTCGGGA CCAATGGAGA GGAACCCAGC CGAGAAGCAT
     5101 CGAGCCGGTA AATGCCGGAA ATTTTTGACT CGAGAGAGAC CATGGCCACC GGTCTCTATC
     5161 GAAGATATGA GGAGCTCAAA CTCGAGctgc taacaaagcc cgaaaggaag ctgagttggc
     5221 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     5281 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
     5341 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5401 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5461 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5521 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5581 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5641 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5701 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5761 aaaatattaa cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5821 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa
     5881 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     5941 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     6001 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     6061 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     6121 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     6181 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     6241 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     6301 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     6361 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     6421 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     6481 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     6541 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     6601 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     6661 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     6721 agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6781 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6841 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6901 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6961 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7021 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7081 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7141 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7201 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7261 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7321 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7381 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7441 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7501 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7561 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     7621 gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7681 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     7741 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     7801 gc
//