LOCUS       pET28a-mH6-Cas12i1      8634 bp ds-DNA     circular     20-NOV-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2515..2542
                     /label="LacO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2497..2515
                     /label="pET28 T7 promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(6710..7522)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      6176..6482
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2670..5954
                     /label="Cas12i1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     RBS             2591..2627
                     /label="pET RBS + 3' UTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      6159..6614
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      7568..8250
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2549..2578
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2628..2669
                     /label="mH6 tag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
       61 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
      121 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
      181 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
      241 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
      301 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
      361 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
      421 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
      481 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
      541 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
      601 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
      661 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
      721 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
      781 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
      841 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
      901 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
      961 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     1021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     1141 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     1201 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     1261 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     1321 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     1381 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     1441 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     1501 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     1561 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     1621 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     1681 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     1741 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     1801 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     1861 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     1921 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     1981 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     2041 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     2101 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     2161 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     2221 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     2281 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     2341 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     2401 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     2461 ccggcgtaga ggatcgagat ctcgatcccg cgaaatTAAT ACGACTCACT ATAGGGGAAT
     2521 TGTGAGCGGA TAACAATTCC CCTCTAGATT TACACTTTAT GCTTCCGGCT CGTATGTTTC
     2581 TAGAAAGCTT AAATAATTTT GTTTAACTTT AAGAAGGAGA TTTAAATatg AAAATCGAAG
     2641 AAGGTAAAGG TCACCATCAC CATCACCACA TGAGCAACAA AGAAAAGAAC GCGAGCGAAA
     2701 CCCGTAAGGC GTACACCACC AAAATGATCC CGCGTAGCCA CGACCGTATG AAGCTGCTGG
     2761 GCAACTTTAT GGACTATCTG ATGGATGGCA CCCCGATTTT CTTTGAACTG TGGAACCAGT
     2821 TCGGTGGCGG TATCGACCGT GATATCATTA GCGGCACCGC GAACAAAGAT AAGATTAGCG
     2881 ACGATCTGCT GCTGGCGGTT AACTGGTTTA AAGTGATGCC GATCAACAGC AAACCGCAAG
     2941 GCGTGAGCCC GAGCAACCTG GCGAACCTGT TCCAGCAATA CAGCGGTAGC GAACCGGACA
     3001 TCCAGGCGCA AGAGTATTTC GCGAGCAACT TTGACACCGA AAAGCACCAG TGGAAAGATA
     3061 TGCGTGTTGA GTACGAACGT CTGCTGGCGG AGCTGCAACT GAGCCGTAGC GACATGCACC
     3121 ATGATCTGAA GCTGATGTAC AAAGAAAAGT GCATTGGCCT GAGCCTGAGC ACCGCGCACT
     3181 ATATCACCAG CGTGATGTTT GGCACCGGTG CGAAGAACAA CCGTCAGACC AAACATCAAT
     3241 TCTATAGCAA AGTGATTCAG CTGCTGGAGG AAAGCACCCA GATCAACAGC GTTGAGCAAC
     3301 TGGCGAGCAT CATTCTGAAA GCGGGTGACT GCGATAGCTA CCGTAAACTG CGTATCCGTT
     3361 GCAGCCGTAA GGGTGCGACC CCGAGCATTC TGAAAATCGT GCAAGACTAT GAACTGGGCA
     3421 CCAACCACGA CGATGAGGTG AACGTTCCGA GCCTGATTGC GAACCTGAAA GAAAAGCTGG
     3481 GCCGTTTTGA GTACGAATGC GAGTGGAAGT GCATGGAGAA AATCAAGGCG TTCCTGGCGA
     3541 GCAAAGTTGG CCCGTACTAT CTGGGTAGCT ATAGCGCGAT GCTGGAGAAC GCGCTGAGCC
     3601 CGATTAAGGG TATGACCACC AAGAACTGCA AATTCGTGCT GAAACAGATT GACGCGAAGA
     3661 ACGATATCAA ATACGAAAAC GAGCCGTTTG GCAAGATCGT TGAAGGTTTC TTTGACAGCC
     3721 CGTATTTCGA GAGCGATACC AACGTGAAAT GGGTTCTGCA CCCGCACCAT ATTGGCGAAA
     3781 GCAACATCAA GACCCTGTGG GAGGACCTGA ACGCGATTCA TAGCAAATAC GAGGAAGACA
     3841 TCGCGAGCCT GAGCGAAGAT AAGAAAGAGA AGCGTATCAA GGTTTACCAG GGCGATGTGT
     3901 GCCAAACCAT CAACACCTAT TGCGAGGAAG TGGGCAAGGA AGCGAAAACC CCGCTGGTTC
     3961 AGCTGCTGCG TTACCTGTAT AGCCGTAAAG ACGATATTGC GGTGGACAAG ATCATTGATG
     4021 GTATCACCTT CCTGAGCAAG AAACACAAGG TGGAGAAACA GAAGATTAAC CCGGTTATCC
     4081 AAAAATACCC GAGCTTCAAC TTTGGCAACA ACAGCAAGCT GCTGGGTAAA ATCATTAGCC
     4141 CGAAAGACAA GCTGAAACAT AACCTGAAGT GCAACCGTAA CCAAGTGGAT AACTACATCT
     4201 GGATTGAAAT CAAGGTTCTG AACACCAAGA CCATGCGTTG GGAGAAACAC CATTATGCGC
     4261 TGAGCAGCAC CCGTTTTCTG GAGGAAGTTT ACTATCCGGC GACCAGCGAA AACCCGCCGG
     4321 ATGCGCTGGC GGCGCGTTTC CGTACCAAAA CCAACGGTTA TGAGGGCAAG CCGGCGCTGA
     4381 GCGCGGAACA GATTGAGCAA ATCCGTAGCG CGCCGGTTGG CCTGCGTAAA GTGAAGAAAC
     4441 GTCAGATGCG TCTGGAAGCG GCGCGTCAGC AAAACCTGCT GCCGCGTTAT ACCTGGGGCA
     4501 AGGATTTTAA CATTAACATC TGCAAACGTG GTAACAACTT CGAAGTTACC CTGGCGACCA
     4561 AGGTGAAGAA AAAGAAAGAG AAGAACTACA AAGTGGTTCT GGGTTATGAC GCGAACATTG
     4621 TGCGTAAAAA CACCTATGCG GCGATTGAGG CGCATGCGAA CGGTGATGGT GTGATTGACT
     4681 ATAACGATCT GCCGGTTAAG CCGATCGAAA GCGGCTTTGT GACCGTTGAG AGCCAGGTTC
     4741 GTGACAAAAG CTACGATCAA CTGAGCTATA ACGGTGTGAA GCTGCTGTAC TGCAAACCGC
     4801 ATGTTGAAAG CCGTCGTAGC TTCCTGGAGA AATATCGTAA CGGCACCATG AAGGACAACC
     4861 GTGGTAACAA CATTCAGATC GACTTCATGA AGGATTTTGA AGCGATCGCG GACGATGAAA
     4921 CCAGCCTGTA CTATTTTAAC ATGAAGTACT GCAAACTGCT GCAGAGCAGC ATTCGTAACC
     4981 ACAGCAGCCA AGCGAAAGAA TATCGTGAGG AAATCTTCGA GCTGCTGCGT GATGGCAAGC
     5041 TGAGCGTGCT GAAACTGAGC AGCCTGAGCA ACCTGAGCTT CGTGATGTTT AAGGTTGCGA
     5101 AAAGCCTGAT TGGCACCTAC TTTGGTCATC TGCTGAAGAA ACCGAAGAAC AGCAAAAGCG
     5161 ACGTTAAGGC TCCGCCGATC ACCGACGAAG ATAAGCAGAA AGCGGACCCG GAGATGTTCG
     5221 CGCTGCGTCT GGCGCTGGAG GAAAAACGTC TGAACAAGGT TAAAAGCAAG AAAGAAGTGA
     5281 TTGCGAACAA GATCGTTGCG AAAGCGCTGG AGCTGCGTGA CAAATACGGC CCGGTGCTGA
     5341 TTAAGGGCGA GAACATCAGC GATACCACCA AGAAAGGTAA GAAAAGCAGC ACCAACAGCT
     5401 TCCTGATGGA CTGGCTGGCG CGTGGCGTGG CGAACAAGGT TAAAGAAATG GTGATGATGC
     5461 ACCAGGGTCT GGAGTTCGTT GAGGTGAACC CGAACTTTAC CAGCCACCAA GATCCGTTCG
     5521 TTCATAAGAA CCCGGAGAAC ACCTTTCGTG CGCGTTACAG CCGTTGCACC CCGAGCGAAC
     5581 TGACCGAGAA GAACCGTAAA GAAATCCTGA GCTTCCTGAG CGACAAGCCG AGCAAACGTC
     5641 CGACCAACGC GTACTATAAC GAGGGTGCGA TGGCGTTTCT GGCGACCTAT GGCCTGAAGA
     5701 AAAACGACGT GCTGGGTGTT AGCCTGGAAA AGTTCAAACA GATTATGGCG AACATCCTGC
     5761 ATCAGCGTAG CGAGGATCAA CTGCTGTTTC CGAGCCGTGG CGGTATGTTC TACCTGGCGA
     5821 CCTATAAGCT GGACGCGGAT GCGACCAGCG TTAACTGGAA CGGCAAACAG TTTTGGGTGT
     5881 GCAACGCGGA CCTGGTTGCG GCGTACAACG TGGGTCTGGT TGACATCCAA AAAGATTTCA
     5941 AGAAAAAGTA ATAAAAGCTT CTCGAGAGAG ACCATGGCCA CCGGTCTCTA TCGAAGATAT
     6001 GAGGAGCTCA AACTCGAGct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     6061 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     6121 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
     6181 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6241 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6301 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6361 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6421 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6481 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6541 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6601 aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6661 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
     6721 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     6781 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     6841 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     6901 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     6961 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     7021 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     7081 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     7141 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     7201 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     7261 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     7321 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     7381 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     7441 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
     7501 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     7561 ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8221 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8281 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8341 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8401 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8461 caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     8521 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     8581 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagc
//