LOCUS Exported 2224 bp ds-DNA circular SYN 04-DEC-2018 DEFINITION MoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes logic).. ACCESSION . VERSION . KEYWORDS P12m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2224) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2224) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 4, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers promoter <5..98 /created_by="User" /Transferred_From="Digestion of pLasTetO - Fragment 2, LasI, LasI PCR Product, p12m, p17, p18, pLas-tetO" /Transferred_Similarity="100.00%" /label="pLas-tetO" operator complement(30..49) /Transferred_From="p58m" /Transferred_Similarity="100.00%" /label="Las Box" protein_bind 75..93 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes; color: #31849b" /X-Transferred_From="tet_operator; tet_operator_(2); tet_operator_(3); tet_operator_(4); tet_operator_(5); tet_operator_(6); tet_operator_(7); tet_operator_(9)" /X-Transferred_Similarity="100.00%" /Transferred_From="AS1_partf.ab1 extraction, P9d" /Transferred_Similarity="100.00%" /label="tet operator" misc_feature complement(111..116) /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" source 1..2224 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 75..93 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 132..152 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(158..175) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(329..917) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(409..428) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1088..1948) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1711..1730 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1949..2053) /gene="bla" /label=AmpR promoter primer_bind 2121..2139 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2143..2186 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2189..2210 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 ggagctagca agggtccggg ttcaccgaaa tctatctcat ttgctagtta taaaattatg 61 aaatttgcgt aaattcccta tcagtgatag agattcagaa gcaattacta gagaccatag 121 tactagagac ctactagtag cggccgctgc aggcttcctc gctcactgac tcgctgcgct 181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 301 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc 361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 961 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 1021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 1081 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 1141 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 1201 ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca gatttatcag 1261 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 1321 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 1381 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 1441 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 1501 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 1561 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 1621 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 1681 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 1741 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 1801 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 1861 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 1921 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 1981 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 2041 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 2101 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat 2161 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt 2221 ctca //