LOCUS       Exported                2182 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly AB part for PlacO (common LacI-repressed 
            promoter; see DOI: 10.1126/science.1256272)..
ACCESSION   .
VERSION     .
KEYWORDS    P32m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2182)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    1..2182
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    5..78
                     /created_by="User"
                     /modified_by="User"
                     /label="PlacO"
     misc_feature    7..23
                     /created_by="User"
                     /label="lacI Operator"
     misc_feature    30..46
                     /created_by="User"
                     /label="lacI Operator"
     misc_feature    83..2182
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     primer_bind     complement(166..186)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190/Part.Seq.REV"
     primer_bind     2078..2098
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     primer_bind     2078..2098
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     source          1..2182
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    7..23
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    30..46
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    90..110
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(116..133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(287..875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(367..386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1046..1906)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1669..1688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1907..2011)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2079..2097
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2101..2144
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2147..2168
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagaattgt gagcggataa caattgacat tgtgagcgga taacaagata ctgagcacat
       61 cagcaggacg cactgaccta ctagagacct actagtagcg gccgctgcag gcttcctcgc
      121 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      181 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
      301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      721 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      961 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1021 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1081 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1141 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac
     1201 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1261 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1321 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1381 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1441 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1501 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1561 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1621 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     1681 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1741 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1801 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1861 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1921 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     1981 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     2041 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     2101 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     2161 ttctagagac tagtgggtct ca
//
