LOCUS Exported 2144 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404).. ACCESSION . VERSION . KEYWORDS P33m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2144) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2144) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..2144 /Original_Bases /label /note="Geneious type: Editing History Insertion" promoter 5..40 /created_by="User" /label="P11" misc_feature 45..2144 /ApEinfo_revcolor="#6ecff6" /ApEinfo_fwdcolor="#6ecff6" /label="DVLA" primer_bind complement(128..148) /Sequence="TTACCGCCTTTGAGTGAGCTG" /Hairpin_Tm="None" /Tm="60.6" /Self_Dimer_Tm="None" /%GC="52.4" /created_by="primer3" /label="SC190/Part.Seq.REV" primer_bind 2040..2060 /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /created_by="primer3" /label="SC189/Part.Seq.FOR" primer_bind 2040..2060 /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /created_by="primer3" /label="SC189/Part.Seq.FOR" source 1..2144 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 52..72 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(78..95) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(249..837) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(329..348) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1008..1868) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1631..1650 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1869..1973) /gene="bla" /label=AmpR promoter primer_bind 2041..2059 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2063..2106 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2109..2130 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 ggagttgaca attaatcatc cggctcttag tgtttgtgga tactagagac ctactagtag 61 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 121 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 181 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 241 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 1021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 1081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 1141 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga 1201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 1261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 1321 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 1381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 1441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 1501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 1561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 1621 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 1681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 1741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 1801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 1861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 1921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 1981 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 2041 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat 2101 gatttctgga attcgcggcc gcttctagag actagtgggt ctca //