LOCUS       Exported                2144 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly AB part (medium-strength Mutalik library 
            promoter; see DOI: 10.1038/nmeth.2404)..
ACCESSION   .
VERSION     .
KEYWORDS    P33m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2144)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    1..2144
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     promoter        5..40
                     /created_by="User"
                     /label="P11"
     misc_feature    45..2144
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     primer_bind     complement(128..148)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190/Part.Seq.REV"
     primer_bind     2040..2060
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     primer_bind     2040..2060
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     source          1..2144
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    52..72
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(78..95)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(249..837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(329..348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1008..1868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1631..1650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1869..1973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2041..2059
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2063..2106
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2109..2130
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagttgaca attaatcatc cggctcttag tgtttgtgga tactagagac ctactagtag
       61 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      121 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      181 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      241 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1141 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1321 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1621 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1981 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2041 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2101 gatttctgga attcgcggcc gcttctagag actagtgggt ctca
//
