LOCUS       Exported                2786 bp ds-DNA     circular SYN 04-DEC-2018
DEFINITION  MoClo golden gate assembly CD part for mRFP (monomeric red 
            fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and 
            https://www.fpbase.org/protein/mrfp1/)..
ACCESSION   .
VERSION     .
KEYWORDS    C5m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2786)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    1..2786
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             2..679
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /NCBI="http://www.ncbi.nlm.nih.gov/protein/BAJ61355.1"
                     /modified_by="Andrey"
                     /label="mRFP"
     misc_feature    687..2786
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     misc_feature    complement(688..693)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(770..790)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190/Part.Seq.REV"
     primer_bind     2682..2702
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     misc_feature    2780..2785
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2780..2785
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     source          1..2786
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2..679
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    694..714
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(720..737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(891..1479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(971..990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1650..2510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2273..2292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2511..2615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2683..2701
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2705..2748
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2751..2772
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggcttcc tccgaagatg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
       61 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
      121 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
      181 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
      241 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
      301 ggacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
      361 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
      421 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gatggtgctc tgaaaggtga
      481 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
      541 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
      601 catcacctcc cacaacgagg actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
      661 tcactccacc ggtgcttaat gaaggtagag acctactagt agcggccgct gcaggcttcc
      721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
      901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg cgacccacgc
     1801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2641 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2701 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2761 ccgcttctag agactagtgg gtctca
//
