LOCUS       Exported                2819 bp ds-DNA     circular SYN 04-DEC-2018
DEFINITION  MoClo golden gate assembly CD part for Citrine (modified YFP; 
            variant of https://www.fpbase.org/protein/citrine/).
ACCESSION   .
VERSION     .
KEYWORDS    C11m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2819)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     CDS             2..712
                     /note="Citrine"
                     /modified_by="Andrey"
                     /label="Citrine"
     misc_feature    complement(265..285)
                     /note="Cit-seq-r_284"
                     /label="Cit-seq-r 284"
     SNP             649
                     /created_by="User"
                     /label="c->t"
     misc_feature    720..2819
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     misc_feature    complement(721..726)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(803..823)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190/Part.Seq.REV"
     primer_bind     2715..2735
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     misc_feature    2813..2818
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2813..2818
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     source          1..2819
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    727..747
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(753..770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(924..1512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1004..1023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1683..2543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2306..2325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2544..2648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2716..2734
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2738..2781
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2784..2805
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg
       61 tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg
      121 taaattgacc ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt
      181 agtcactact ttaggttatg gtttgatgtg ttttgctaga tacccagatc atatgaaaca
      241 acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt
      301 caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt
      361 taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa
      421 attggaatac aactataact ctcacaatgt ttacatcatg gctgacaaac aaaagaatgg
      481 tatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggttctgttc aattagctga
      541 ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta
      601 cttatcctat caatctagat tatccaaaga tccaaacgaa aagagagatc acatggtctt
      661 gttagaattt gttactgctg ctggtattac ccatggtatg gatgaattgt acaaaaggta
      721 gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc
      781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      901 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1801 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2641 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2701 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2761 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctca
//
