LOCUS       Exported                2864 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP 
            with ssrA degradation tag; variant of 
            https://www.fpbase.org/protein/yfp/).
ACCESSION   .
VERSION     .
KEYWORDS    C20m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2864)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    1..2864
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     Junction        join(4,8)
                     /Reads_using=2
                     /Reads_supporting_discovery=2
                     /Junction_Type="Deletion"
                     /Deletion_Size=3
                     /Junction_Source="4+"
                     /Junction_Destination="8+"
                     /ID="junction 1"
                     /label="junction 1"
     CDS             5..760
                     /created_by="User"
                     /label="sfYFP ssrA"
     misc_feature    join(5..610,614..718)
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="superfolder GFP->YFP"
     misc_feature    200..202
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Y66"
     misc_feature    611..613
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="T203Y YFP"
     misc_feature    719..760
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="#ff8040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="LAA"
     misc_feature    765..2864
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     primer_bind     complement(848..868)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190/Part.Seq.REV"
     primer_bind     2760..2780
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     source          1..2864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             5..718
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    772..792
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(798..815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(969..1557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1049..1068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1728..2588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2351..2370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2589..2693)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2761..2779
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2783..2826
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2829..2850
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga
       61 tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa
      121 tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac
      181 tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa
      241 gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc
      301 ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct
      361 ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca
      421 taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa
      481 tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc
      541 tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca
      601 ctatctgagc taccaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt
      661 tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaaac
      721 tagtgcagcg aacgacgaaa attacgccct tgcagcgtga aggtagagac ctactagtag
      781 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      841 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      901 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      961 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1021 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1081 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1141 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1201 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1261 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1321 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1381 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1441 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1501 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1561 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1621 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1681 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1741 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1801 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1861 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1921 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1981 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2041 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2101 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2161 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2221 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2281 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2341 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2401 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2461 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2521 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2581 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2641 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2701 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2761 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2821 gatttctgga attcgcggcc gcttctagag actagtgggt ctca
//
