LOCUS Exported 2819 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version).. ACCESSION . VERSION . KEYWORDS C24m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2819) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2819) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..2819 /Original_Bases /label /note="Geneious type: Editing History Insertion" CDS <5..715 /note="Oriinal location: 2359..3105" /note="lambda repressor (cI) + destruction tag" /label="cI-boom" misc_feature 720..2819 /ApEinfo_revcolor="#6ecff6" /ApEinfo_fwdcolor="#6ecff6" /label="DVLA" misc_feature complement(721..726) /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" primer_bind complement(803..823) /Sequence="TTACCGCCTTTGAGTGAGCTG" /Hairpin_Tm="None" /Tm="60.6" /Self_Dimer_Tm="None" /%GC="52.4" /created_by="primer3" /label="SC190/Part.Seq.REV" primer_bind 2715..2735 /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /created_by="primer3" /label="SC189/Part.Seq.FOR" misc_feature 2813..2818 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" misc_feature 2813..2818 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" source 1..2819 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 2..715 /codon_start=1 /gene="cI" /product="phage lambda repressor" /label=-lambda repressor /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV VGKVIASQWPEETFG" misc_feature 727..747 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(753..770) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(924..1512) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1004..1023) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1683..2543) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2306..2325 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2544..2648) /gene="bla" /label=AmpR promoter primer_bind 2716..2734 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2738..2781 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2784..2805 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa 61 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa 121 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc 181 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc 241 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag 301 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag 361 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc 421 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag 481 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt 541 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg 601 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg 661 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgaaggta 721 gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc 781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 901 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa 961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 1021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 1081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 1141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 1201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 1261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 1321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 1381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 1441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 1501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 1561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 1621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 1681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 1741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 1801 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata 1861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 1921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 1981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 2041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 2101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 2161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 2221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 2281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 2341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 2401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 2461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 2521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 2581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 2641 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 2701 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta 2761 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctca //