LOCUS Exported 3606 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5.. ACCESSION . VERSION . KEYWORDS C31m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3606) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 3606) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers promoter complement(635..704) /Color="#ffffff" /Transferred_From="AmpR_promoter_(4)" /Transferred_Similarity="100.00%" /label="AmpR promoter" promoter 3025..3054 /Color="#ffffff" /Transferred_From="RNA_I_promoter" /Transferred_Similarity="100.00%" /label="RNA I promoter" promoter complement(3179..3207) /Color="#ffffff" /Transferred_From="RNAII_promoter" /Transferred_Similarity="100.00%" /label="RNAII promoter" CDS complement(3378..632) /Color="#ccffcc" /X-Transferred_From="AmpR; AmpR_(3); AmpR_(4)" /X-Transferred_Similarity="99.65% -> 98.95%" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /label="AmpR" CDS complement(3378..632) /Color="#ccffcc" /X-Transferred_From="AmpR; AmpR_(3); AmpR_(4)" /X-Transferred_Similarity="99.65% -> 98.95%" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /label="AmpR" source 1..3606 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 395..414 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(633..737) /gene="bla" /label=AmpR promoter primer_bind 805..823 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 827..870 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 873..894 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" primer_bind complement(2448..2465) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2619..3207) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2699..2718) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3378..632) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 61 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 781 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa 841 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagg 901 tctcaaatgg tgagagccct ggtagtcatc cgcctgtccc gcgtcaccga tgctacgact 961 tcaccggagc gtcagctgga gtcttgccag cagctctgcg cccagcgcgg ctgggacgtc 1021 gtcggggtag cggaggatct ggacgtctcc ggggcggtcg atccgttcga ccggaagcgc 1081 agaccgaacc tggcccggtg gctagcgttc gaggagcaac cgtttgacgt gatcgtggcg 1141 taccgggtag atcggttgac ccgatcgatc cggcatcttc agcagctggt ccactgggcc 1201 gaggaccaca agaagctggt cgtctccgcg accgaagcgc acttcgatac gacgacgccg 1261 tttgcggcgg tcgtcatcgc gcttatggga acggtggcgc agatggaatt agaagcgatc 1321 aaagagcgga accgttcggc tgcgcatttc aatatccgcg ccgggaaata ccgaggatcc 1381 ctgccgccgt ggggatacct gcctacgcgc gtggacgggg agtggcggct ggtgccggac 1441 cctgtgcagc gagagcgcat cctcgaggtg tatcaccgcg tcgtcgacaa ccacgagccg 1501 ctgcatctgg tggcccacga cctgaaccgg cgtggtgtcc tgtcgccgaa ggactacttc 1561 gcgcagctgc aaggccgcga gccgcagggc cgggagtggt cggctaccgc gctgaagcga 1621 tcgatgatct ccggggcgat gctcgggtac gcgactctga acggtaagac cgtccgagac 1681 gacgacggag ccccgctggt gcgggctgag ccgatcctga cccgtgagca gctggaggcg 1741 ctgcgcgccg agctcgtcaa gacctcccgg gcgaagcccg cggtgtctac cccgtcgctg 1801 ctgctgcggg tgttgttctg cgcggtgtgc ggggagcccg cgtacaagtt cgccggggga 1861 ggacgtaagc acccgcgcta ccgctgccgc tcgatggggt tcccgaagca ctgcgggaac 1921 ggcacggtgg cgatggccga gtgggacgcg ttctgcgagg agcaggtact ggatctgctc 1981 ggggacgcgg agcgtctgga gaaagtctgg gtagcgggct cggactccgc ggtcgaactc 2041 gcggaggtga acgcggagct ggtggacctg acgtcgctga tcggctcccc ggcctaccgg 2101 gcgggctctc cgcagcgaga agcactggat gcccgtattg cggcgctggc cgcgcggcaa 2161 gaggagctgg agggcctgga ggctcgcccg tctggctggg agtggcgcga aaccgggcag 2221 cggttcgggg actggtggcg ggagcaggac accgcggcaa agaacacctg gcttcggtcg 2281 atgaacgttc ggctgacgtt cgacgtccgc ggcgggctga ctcgcacgat cgacttcggg 2341 gatcttcagg agtacgagca gcatctcagg ctcggcagcg tggtcgaacg gctacacacc 2401 gggatgtcgt aaaggttgag accctagtag cggccgctgc aggcttcctc gctcactgac 2461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 2521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 2581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct 2641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 2701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 2761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 2821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 2881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 2941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 3061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 3301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3361 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 3421 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 3481 ggcttaccat ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca 3541 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 3601 ttatcc //