LOCUS       Exported                2752 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly CD part for RhlI (quorum-sensing synthase
            for 3OC14)..
ACCESSION   .
VERSION     .
KEYWORDS    C36m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2752)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     ligation        1..4
                     /label="Ligation"
     CDS             2..604
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="C165_pLLacBCD2ms (modified),
                     C216_pRhl17LacOs (modified), C247_pRhlo17LacO (modified)"
                     /Transferred_Similarity="100.00%"
                     /label="RhlI from PAO1"
     misc_feature    5..24
                     /Mismatches=0
                     /Extension="TAGTGGAAGACGTAATG"
                     /created_by="primer3"
                     /annotation_group="281738780596554a8ba690a-5d0d-4004-8922-
                     ea495bdcafd5"
                     /%GC="40.0"
                     /Tm="53.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATCGAATTGCTCTCTGAATC"
                     /label="pr41a GG RhlI Fwd ohC"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    74..248
                     /Source="referenceToDocument: urn:local:.:6s-9u0niyb"
                     /label="Digestion of RhlI - Fragment 3 (concatenated
                     sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        74..77
                     /label="Ligation"
     misc_feature    complement(80..85)
                     /Recognition_pattern="GAAGAC(2/6)"
                     /annotation_group="GAAGAC"
                     /label="BbsI"
                     /note="Geneious type: restriction site"
     misc_feature    109..114
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    137..142
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    245..652
                     /Source="referenceToDocument: urn:local:.:6s-9u0niyb"
                     /label="Digestion of RhlI - Fragment 4 (concatenated
                     sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     ligation        245..248
                     /label="Ligation"
     misc_feature    complement(251..256)
                     /Recognition_pattern="GAAGAC(2/6)"
                     /annotation_group="GAAGAC"
                     /label="BbsI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(497..502)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     sig_peptide     611..643
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_label="ssrA"
                     /Transferred_From="C165_pLLacBCD2ms (modified),
                     C205a_pRhlLacsfC (modified), C216_pRhl17LacOs (modified),
                     C217_pCino17LacO (modified), C235_pCino17tetm (modified),
                     C247_pRhlo17LacO (modified)"
                     /Transferred_Similarity="100.00%"
                     /label="ssrA; ssrA(1)"
     misc_feature    649..77
                     /label="Digestion of DVA_CD (modified) - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        649..652
                     /label="Ligation"
     sig_peptide     653..2752
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     misc_feature    complement(654..659)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    661..666
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI"
                     /note="Geneious type: restriction site"
     misc_feature    2739..2744
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI"
                     /note="Geneious type: restriction site"
     misc_feature    2746..2751
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2746..2751
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     source          1..2752
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    660..680
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(686..703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(857..1445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(937..956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1616..2476)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2239..2258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2477..2581)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2649..2667
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2671..2714
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2717..2738
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatcgaa ttgctctctg aatcgctgga agggctttcc gccgccatga tcgccgagct
       61 gggacgctac cggcatcagg tcttcatcga gaagctgggc tgggacgtgg tctccacctc
      121 cagggtccgc gaccaggaat tcgaccagtt cgaccatccg caaacccgct acatcgtcgc
      181 catgagccgc cagggcatct gcggttgcgc ccgcctgctg ccgacgaccg acgcctacct
      241 gctcaaggac gtcttcgcct acctgtgcag cgaaaccccg ccgagcgatc cgtcggtctg
      301 ggagctttcg cgctacgccg ccagcgcggc ggacgatccg cagctggcga tgaagatatt
      361 ctggtccagc ctgcaatgcg cctggtacct gggcgccagt tcggtggtgg cggtgaccac
      421 cacggccatg gagcgctatt tcgttcgcaa cggcgtgatc ctccagcgcc tcggcccgcc
      481 gcagaaggtc aagggcgaga cgctggtcgc gatcagcttc ccggcctacc aggagcgcgg
      541 cctggagatg ctgctgcgct accacccgga atggctgcag ggcgtaccgc tgtcgatggc
      601 ggtgacctcc gcagcgaacg acgaaaatta cgcccttgca gcgtaataag gtagagacct
      661 actagtagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      841 ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac
      901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1441 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1501 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1561 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1621 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1681 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1741 ctgcaatgat accgcgcgac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1801 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1861 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1921 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1981 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2041 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2101 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2161 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2221 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2281 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2341 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2401 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2461 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2521 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2581 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2641 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     2701 ctaaggatga tttctggaat tcgcggccgc ttctagagac tagtgggtct ca
//
