LOCUS       Exported                3101 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase 
            active at transition from exponential phase to stationary phase). .
ACCESSION   .
VERSION     .
KEYWORDS    C38m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3101)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    1..3101
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             5..997
                     /created_by="User"
                     /modified_by="User"
                     /label="RpoS"
     misc_feature    1002..3101
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     primer_bind     complement(1085..1105)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190/Part.Seq.REV"
     primer_bind     2997..3017
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     primer_bind     2997..3017
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     source          1..3101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1009..1029
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1035..1052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1206..1794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1286..1305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1965..2825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2588..2607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2826..2930)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2998..3016
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      3020..3063
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3066..3087
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgagt cagaatacgc tgaaagttca tgatttaaat gaagatgcgg aatttgatga
       61 gaacggagtt gaggtttttg acgaaaaggc cttagtagaa caggaaccca gtgataacga
      121 tttggccgaa gaggaactgt tatcgcaggg agccacacag cgtgtgttgg acgcgactca
      181 gctttacctt ggtgagattg gttattcacc actgttaacg gccgaagaag aagtttattt
      241 tgcgcgtcgc gcactgcgtg gagatgtcgc ctctcgccgc cggatgatcg agagtaactt
      301 gcgtctggtg gtaaaaattg cccgccgtta tggcaatcgt ggtctggcgt tgctggacct
      361 tatcgaagag ggcaacctgg ggctgatccg cgcggtagag aagtttgacc cggaacgtgg
      421 tttccgcttc tcaacatacg caacctggtg gattcgccag acgattgaac gggcgattat
      481 gaaccaaacc cgtactattc gtttgccgat tcacatcgta aaggagctga acgtttacct
      541 gcgaaccgca cgtgagttgt cccataagct ggaccatgaa ccaagtgcgg aagagatcgc
      601 agagcaactg gataagccag ttgatgacgt cagccgtatg cttcgtctta acgagcgcat
      661 tacctcggta gacaccccgc tgggtggtga ttccgaaaaa gcgttgctgg acatcctggc
      721 cgatgaaaaa gagaacggtc cggaagatac cacgcaagat gacgatatga agcagagcat
      781 cgtcaaatgg ctgttcgagc tgaacgccaa acagcgtgaa gtgctggcac gtcgattcgg
      841 tttgctgggg tacgaagcgg caacactgga agatgtaggt cgtgaaattg gcctcacccg
      901 tgaacgtgtt cgccagattc aggttgaagg cctgcgccgt ttgcgcgaaa tcctgcaaac
      961 gcaggggctg aatatcgaag cgctgttccg cgagtaaagg tagagaccta ctagtagcgg
     1021 ccgctgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1201 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1741 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1921 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1981 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2041 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2101 ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2161 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2221 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2281 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2341 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2401 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2461 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2521 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2581 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2641 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2701 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2761 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2821 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2941 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     3001 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
     3061 ttctggaatt cgcggccgct tctagagact agtgggtctc a
//
