LOCUS       Exported                2825 bp ds-DNA     circular SYN 04-DEC-2018
DEFINITION  MoClo golden gate assembly CD part for sfYFP (superfolder YFP; 
            variant of https://www.fpbase.org/protein/yfp/.
ACCESSION   .
VERSION     .
KEYWORDS    C51m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2825)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     ligation        1..4
                     /label="Ligation"
     CDS             2..>715
                     /created_by="User"
                     /modified_by="Reed McCardell"
                     /label="sfYFP"
     misc_feature    join(2..607,611..715)
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="superfolder GFP->YFP"
     misc_feature    197..199
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Y66"
     misc_feature    608..610
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="T203Y YFP"
     misc_feature    complement(692..715)
                     /Mismatches=0
                     /Extension="AGGTCTGGTCTCAACCTTCATTA"
                     /created_by="primer3"
                     /annotation_group="283081391378767840b0ca2-4908-4a83-a9f3-
                     012499c00c3f"
                     /%GC="37.5"
                     /Tm="57.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTTGTACAGTTCATCCATACCATG"
                     /Product_Size=714
                     /label="BsaAGGT_sfYFP_end_R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    716..>725
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        722..725
                     /label="Ligation"
     misc_feature    726..2825
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     misc_feature    complement(809..829)
                     /Transferred_From="pVHgc_1"
                     /Transferred_Similarity="100.00%"
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /modified_by="User"
                     /label="Moclo_seq_R"
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(1689..2549)
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="bla"
     primer_bind     2721..2741
                     /Transferred_From="pVHgc_1"
                     /Transferred_Similarity="100.00%"
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /modified_by="User"
                     /label="Moclo_seq_F"
     misc_feature    2813..1
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    2813..1
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     source          1..2825
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2..715
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    733..753
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(759..776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(930..1518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1010..1029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1689..2549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2312..2331
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2550..2654)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2722..2740
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2744..2787
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2790..2811
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg
       61 tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg
      121 taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct
      181 ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca
      241 gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt
      301 taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt
      361 aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc tgggccataa
      421 gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg
      481 cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga
      541 tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta
      601 tctgagctac caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct
      661 gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaaataatg
      721 aaggtagaga cctactagta gcggccgctg caggcttcct cgctcactga ctcgctgcgc
      781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      901 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
      961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1801 tctggcccca gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca
     1861 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     1981 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2281 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2641 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2701 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     2761 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga gactagtggg
     2821 tctca
//
