LOCUS       Exported                2804 bp ds-DNA     circular SYN 04-DEC-2018
DEFINITION  MoClo golden gate assembly CD part for mScarlet (RFP variant; see 
            https://www.fpbase.org/protein/mscarlet/)..
ACCESSION   .
VERSION     .
KEYWORDS    C59m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2804)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     CDS             2..706
                     /created_by="User"
                     /modified_by="Andrey"
                     /label="mScarlet"
     misc_feature    5..700
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    705..2804
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     misc_feature    complement(706..711)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(788..808)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190/Part.Seq.REV"
     primer_bind     2700..2720
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     misc_feature    2798..2803
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2798..2803
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     source          1..2804
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             5..700
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(138..156)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    712..732
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(738..755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(909..1497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(989..1008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1668..2528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2291..2310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2529..2633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2701..2719
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2723..2766
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2769..2790
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatggtg agcaagggcg aggcagtgat caaggagttc atgcggttca aggtgcacat
       61 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      121 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttctcctg
      181 ggacatcctg tcccctcagt tcatgtacgg ctccagggcc ttcaccaagc accccgccga
      241 catccccgac tactataagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      301 cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct
      361 gatctacaag gtgaagctcc gcggcaccaa cttccctcct gacggccccg taatgcagaa
      421 gaaaacaatg ggctgggaag cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa
      481 gggcgacatt aagatggccc tgcgcctgaa ggacggcggc cgctacctgg cggacttcaa
      541 gaccacctac aaggccaaga agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa
      601 gttggacatc acctcccaca acgaggacta caccgtggtg gaacagtacg aacgctccga
      661 gggccgccac tccaccggcg gcatggacga gctgtacaag aggtagagac ctactagtag
      721 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      901 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1801 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2641 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2701 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2761 gatttctgga attcgcggcc gcttctagag actagtgggt ctca
//
