LOCUS Exported 4379 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag).. ACCESSION . VERSION . KEYWORDS C67m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4379) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 4379) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers misc_feature complement(128..145) /Sequence="GGAGACGTCCAGATCCTC" /Hairpin_Tm="43.3" /Tm="66.5" /Self_Dimer_Tm="13.0" /%GC="61.1" /modified_by="User" /label="Bxb1_seq_R" /note="Geneious type: primer_bind_reverse" CDS complement(3243..4103) /ApEinfo_revcolor="#faac61" /ApEinfo_fwdcolor="#faac61" /label="bla" primer_bind 4275..4295 /Transferred_From="pVHgc_1" /Transferred_Similarity="100.00%" /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /modified_by="User" /label="Moclo_seq_F" primer_bind 4275..4295 /Transferred_From="pVHgc_1" /Transferred_Similarity="100.00%" /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /modified_by="User" /label="Moclo_seq_F" source 1..4379 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 2287..2307 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(2313..2330) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2484..3072) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2564..2583) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3243..4103) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3866..3885 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4104..4208) /gene="bla" /label=AmpR promoter primer_bind 4276..4294 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 4298..4341 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 4344..4365 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta cgacttcacc 61 ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg acgtcgtcgg 121 ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga agcgcagacc 181 gaacctggcc cggtggctag cgttcgagga gcaaccgttt gacgtgatcg tggcgtaccg 241 ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact gggccgagga 301 ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga cgccgtttgc 361 ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag cgatcaaaga 421 gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag gatccctgcc 481 gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt 541 gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca 601 tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca 661 gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga agcgatcgat 721 gatctccggg gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc gagacgacga 781 cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg aggcgctgcg 841 cgccgagctc gtcaagacct cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct 901 gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg 961 taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg ggaacggcac 1021 ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc tgctcgggga 1081 cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg aactcgcgga 1141 ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct accgggcggg 1201 ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc ggcaagagga 1261 gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgaaaccg ggcagcggtt 1321 cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa 1381 cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact tcggggatct 1441 tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac acaccgggat 1501 gtcgggtagc ggcagcggta gcggtagtgg gagcggcagc atggtcagta aaggcgaagc 1561 agttatcaaa gagttcatgc gcttcaaagt tcatatggaa gggtcgatga acgggcacga 1621 atttgaaatt gaaggcgaag gcgaaggccg cccatatgaa gggacccaaa ccgcaaagct 1681 taaggttact aaaggcggtc cattaccctt ttcgtgggac attttaagcc cacagtttat 1741 gtacgggagt cgcgctttca tcaagcaccc tgcggacatc ccagattact acaaacagtc 1801 tttccccgag gggttcaagt gggagcgcgt gatgaacttc gaggatggcg gagccgtgac 1861 ggtcacccaa gatacctctt tggaggacgg tacgttgatc tacaaagtga aattgcgtgg 1921 cacgaatttt ccacctgatg ggcctgtcat gcagaaaaag acaatgggat gggaagcttc 1981 cacggagcgc ctttacccag aggacggtgt tcttaaaggg gatatcaaaa tggcgctgcg 2041 tcttaaagat ggaggccgct acctggcgga cttcaagact acttacaagg ccaaaaaacc 2101 agtgcagatg ccgggtgcgt acaatgtaga tcgtaaatta gatattacaa gtcacaatga 2161 agattacacg gtcgtagagc agtatgagcg cagtgagggg cgtcactcta cgggcggtat 2221 ggacgagtta tacaaggcgg ctaacgacga gaattatgca ttggccgcct aataaaggta 2281 gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc 2341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 2401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 2461 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa 2521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 2581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 2641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 2701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 2761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 2821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 2881 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 2941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 3001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 3061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 3121 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 3181 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 3241 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 3301 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 3361 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata 3421 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 3481 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 3541 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 3601 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 3661 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 3721 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 3781 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 3841 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 3901 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 3961 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 4021 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 4081 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 4141 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 4201 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 4261 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta 4321 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctca //