LOCUS Exported 2836 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).. ACCESSION . VERSION . KEYWORDS C70m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2836) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2836) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2836 /organism="recombinant plasmid" /mol_type="other DNA" source 1..2836 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(154..1014) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 777..796 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1015..1119) /gene="bla" /label=AmpR promoter primer_bind 1187..1205 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1209..1252 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 1255..1276 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 2034..2054 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(2060..2077) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2231..2819) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2311..2330) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 61 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc 301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 1021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 1081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 1141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 1201 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc 1261 gcggccgctt ctagagacta gtgggtctca aatgattgtg ggtgaagatc agctgtgggg 1321 tcgtcgtaca ctggaatttg ttgatagcgt tgaacgtctg gaagcaccgg cactgattag 1381 ccgttttgaa agcctgattg caagctgtgg ttttaccgcc tatatcatgg caggtctgcc 1441 gagccgtaat gccggtctgc cggaactgac cctggcaaat ggttggcctc gtgattggtt 1501 tgatctgtat gttagcgaaa actttagcgc agttgatccg gttccgcgtt atggtgcaac 1561 cactgttcat ccgtttgttt ggagtgatgc accgtatgat cgtgaccgtg atcaggcagc 1621 acatcgtgtt atgacccgtg cagcagaatt tggtctggtt gaaggttatt gtattccgct 1681 gcattacgat gatggtagcg cagcaattag tatggcaggt gaagatcctg atctgagtcc 1741 ggcagcccgt ggtgtaatgc agctggttag catttatgca catagccgtc tgcgtgtact 1801 gagccgtccg aaaccgattc gtcgtaatcg tctgacaccg cgtgaatgtg aaattctgca 1861 gtgggcagca cagggtaaaa ccgcatggga aattagcgtt attctgtgta ttaccgaacg 1921 caccgttaaa tttcatctga ttgaagcagc acgtaaactg gatgcagcaa atcgtaccgc 1981 agcagttgca aaagcactga cactgggtct gattcgtctg tgaggtagag acctactagt 2041 agcggccgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 2101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 2161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 2221 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 2281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 2341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 2401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 2461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 2521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 2581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 2641 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 2701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 2761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 2821 aagatccttt gatctt //