LOCUS Exported 3007 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD part for NahRQ-VHAC (Adam Meyer's improved NahR activator from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).. ACCESSION . VERSION . KEYWORDS C75m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3007) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 3007) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3007 /organism="recombinant plasmid" /mol_type="other DNA" promoter 2808..2837 /label="RNA I promoter" promoter 2808..2837 /label="RNA I promoter" source 1..3007 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(154..1014) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 777..796 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1015..1119) /gene="bla" /label=AmpR promoter primer_bind 1187..1205 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1209..1252 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 1255..1276 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 2205..2225 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(2231..2248) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2402..2990) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2482..2501) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 61 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc 301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 1021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 1081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 1141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 1201 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc 1261 gcggccgctt ctagagacta gtgggtctca aatggaactg cgtgacctgg atttaaacct 1321 gctggtggtg ttcaaccagt tgctggtcga cagacgcgtc tctgtcactg cggagaacct 1381 gggcctgacc cagcctgccg tgagcaatgc gctgaaacgc ctgcgcacct cgctacagga 1441 cccactcttc gtgcgcacac atcagggaat ggaacccaca ccctatgccg cgcatctggc 1501 cgagcacgtc acttcggcca tgcacgcact gcgcaacgcc ctacagcacc atgaaagctt 1561 cgatccgctg accagcgagc gtaccttcac cctggccatg accgacattg gcgagatcta 1621 cttcatgccg cggctgatgg atgcgctggc tcaccaggcc cccaattgcg tgatcagtac 1681 ggtgcgcgac agttcgatga gcctgatgca ggccttgcag aacggaaccg tggacttggc 1741 cgtgggcctg cttcccaatc tgcaaactgg cttctttcag cgccggctgc tccagaatca 1801 ctacgtgtgc ctatgtcgca aggaccatcc agtcacccgc gaacccctga ctctggagcg 1861 cttctgttcc tacggccacg tgcgtgtcat cgccgctggc accggccacg gcgaggtgga 1921 cacgtacatg acacgggtcg gcatccggcg cgacatccgt ctggaagtgc cgcacttcgc 1981 cgccgttggc cacatcctcc agcgcaccga tctgctcgcc actgtgccga tatgtttagc 2041 cgactgctgc gtagagccct tcggcctaag cgccttgccg cacccagtcg tcttgcctga 2101 aatagccatc aacatgttct ggcatgcgaa gtaccacaag gacctagcca atatttggtt 2161 gcggcaactg atgtttgacc tgtttacgga ttgaggtaga gacctactag tagcggccgc 2221 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 2281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 2341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 2401 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 2461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 2521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 2581 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 2641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 2701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 2761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 2821 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 2881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 2941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 3001 tgatctt //