LOCUS Exported 2969 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein).. ACCESSION . VERSION . KEYWORDS C77m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2969) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2969) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers overhang 1..4 /created_by="User" /label="C overhang" misc_feature 2963..2968 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" misc_feature 2963..2968 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" source 1..2969 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 5..865 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 877..897 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(903..920) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1074..1662) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1154..1173) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1833..2693) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2694..2798) /gene="bla" /label=AmpR promoter primer_bind 2866..2884 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2888..2931 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2934..2955 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatgatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 61 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 121 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 181 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 241 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 301 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 361 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 421 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 481 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 541 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 601 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 661 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 721 gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 781 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 841 tgcctcactg attaagcatt ggtaaaggta gagacctact agtagcggcc gctgcaggct 901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac 1081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 1141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 1201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 1261 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 1321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1501 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 1681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1741 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 1801 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 1861 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 1921 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgcgaccca 1981 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 2041 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 2101 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 2161 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 2221 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 2281 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 2341 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 2401 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 2461 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 2521 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 2581 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 2641 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 2701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 2761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 2821 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 2881 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg 2941 cggccgcttc tagagactag tgggtctca //