LOCUS       Exported                2969 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin
            resistance protein)..
ACCESSION   .
VERSION     .
KEYWORDS    C77m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2969)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     overhang        1..4
                     /created_by="User"
                     /label="C overhang"
     misc_feature    2963..2968
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2963..2968
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     source          1..2969
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             5..865
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    877..897
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(903..920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1074..1662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1154..1173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1833..2693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2694..2798)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2866..2884
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2888..2931
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2934..2955
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
       61 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      121 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      181 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      241 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      301 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      361 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      421 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      481 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      541 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      601 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      661 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      721 gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      781 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      841 tgcctcactg attaagcatt ggtaaaggta gagacctact agtagcggcc gctgcaggct
      901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
     1081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1261 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1501 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1741 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1801 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1861 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1921 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgcgaccca
     1981 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2041 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2101 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2161 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2221 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2281 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2341 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2401 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2461 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2521 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2581 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2641 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2821 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2881 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
     2941 cggccgcttc tagagactag tgggtctca
//
