LOCUS       Exported                2768 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly CD cloning part for ChlorR 
            (chloramphenicol resistance protein)..
ACCESSION   .
VERSION     .
KEYWORDS    C78m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2768)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     overhang        1..4
                     /created_by="User"
                     /label="C overhang"
     resistance      5..664
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /modified_by="User"
                     /label="ChlorR / CmR"
     misc_feature    8..664
                     /Original_Bases="TCTTCTGCACCATATGCGGTGTGAAATACCGCACAGATGCG
                     TAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGA
                     AGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCT
                     GCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGA
                     CGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATG
                     CAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCAC
                     AATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGA
                     GTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACC
                     TGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAAGACGT"
                     /label="TCTTCTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAA
                     AATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATC
                     GGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGA
                     TTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTG
                     AATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGG
                     CGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACA
                     CAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA
                     CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCC
                     AGCTGCATTAATGAATCGGCCAACGCGCGGGGAAGACGT"
                     /note="Geneious type: Editing History Replacement"
     overhang        complement(665..668)
                     /created_by="User"
                     /label="D overhang"
     misc_feature    669..2768
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     misc_feature    complement(670..675)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2762..2767
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2762..2767
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     source          1..2768
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             5..664
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(71..90)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     misc_feature    676..696
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(702..719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(873..1461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(953..972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1632..2492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2255..2274
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2493..2597)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2665..2683
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2687..2730
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2733..2754
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
       61 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
      121 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
      181 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga
      241 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
      301 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
      361 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
      421 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
      481 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
      541 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
      601 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
      661 gtaaaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg
      721 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      781 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      841 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
      901 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      961 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1021 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1081 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1141 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1201 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1261 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1321 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1381 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1441 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1501 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1561 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1621 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1681 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1741 ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta
     1801 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1861 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1921 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1981 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2041 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2101 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2161 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2221 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2281 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2341 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2401 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2461 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2521 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2581 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2641 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     2701 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagt
     2761 gggtctca
//
