LOCUS Exported 2762 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD part for RpaI (synthase for Rpa-detected quorum sensing molecule 4-coumaroyl-homoserine lactone). . ACCESSION . VERSION . KEYWORDS C82m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2762) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2762) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers misc_feature 2743..2080 /ApEinfo_revcolor="#6ecff6" /ApEinfo_fwdcolor="#6ecff6" /label="DVLA" primer_bind complement(64..84) /Sequence="TTACCGCCTTTGAGTGAGCTG" /Hairpin_Tm="None" /Tm="60.6" /Self_Dimer_Tm="None" /%GC="52.4" /created_by="primer3" /label="SC190/Part.Seq.REV" primer_bind 1976..1996 /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /created_by="primer3" /label="SC189/Part.Seq.FOR" CDS 2082..2735 /ApEinfo_fwdcolor="#40a6ff" /ApEinfo_revcolor="#40a6ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}" /modified_by="anandhswaminathan" /label="RpaI" CDS 2082..2735 /ApEinfo_fwdcolor="#40a6ff" /ApEinfo_revcolor="#40a6ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}" /modified_by="anandhswaminathan" /label="RpaI" source 1..2762 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(14..31) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(185..773) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(265..284) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(944..1804) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1567..1586 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1805..1909) /gene="bla" /label=AmpR promoter primer_bind 1977..1995 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1999..2042 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2045..2066 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 2750..8 /label=BioBrick suffix /note="universal suffix for all parts" ORIGIN 1 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 61 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 181 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1081 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 1261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 1321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 1381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 1441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 1501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 1561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 1621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 1681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 1741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 1801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 1861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 1921 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 1981 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 2041 tctggaattc gcggccgctt ctagagacta gtgggtctca aatgcaggtt catgtcatcc 2101 gtcgagagaa ccgcgcgctc tatgccggtc tgctcgaaaa gtacttccgc atccgtcacc 2161 agatctacgt cgtcgagcgc ggctggaagg agctcgatcg gccggatggc cgcgagatcg 2221 atcagttcga caccgaagac gccgtgtatc tgctcggcgt cgacaatgac gacatcgtcg 2281 ccggcatgcg gatggtgccg accacgtcac cgacgctcct cagcgacgtc ttcccgcagc 2341 ttgcgctggc aggcccggtg cggcggccgg atgcctacga gctgtcgcgg atcttcgtgg 2401 taccgcgcaa gcgcggcgag catggcggcc cgcgcgccga agccgtgatc caggcggccg 2461 cgatggagta cggcctgtcg atcggtctgt cggccttcac catcgtgctg gagacctggt 2521 ggctgccgcg actggtggac cagggctgga aggcaaagcc gctcggcctg cctcaggaca 2581 tcaacggatt ctcgaccacc gcagtgatcg tcgacgtcga cgacgacgcc tgggtcggca 2641 tctgcaatcg ccgctcggtg cccggaccca cgctggaatg gcgcgggctc gaagccatcc 2701 gccgtcattc gcttccggaa ttccaggtga tttcataaag gtagagacct actagtagcg 2761 gc //