LOCUS       Exported                3023 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly CD part for pir (Initiation factor 
            required for replication of R6K origin plasmids)..
ACCESSION   .
VERSION     .
KEYWORDS    C83m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3023)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    2537..2557
                     /Color="#a6ccff"
                     /Transferred_From="BioBrick_suffix"
                     /Transferred_Similarity="100.00%"
                     /label="BioBrick suffix"
     primer_bind     complement(2613..2633)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /modified_by="andrey"
                     /Transferred_From="Part.Seq.REV"
                     /Transferred_Similarity="100.00%"
                     /label="Part.Seq.REV"
     source          1..3023
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(470..1330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1093..1112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1331..1435)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1503..1521
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1525..1568
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1571..1592
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2537..2557
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2563..2580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2734..299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2814..2833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
       61 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      121 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      181 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      241 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
      301 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
      361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      481 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      541 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      601 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      661 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      721 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      781 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      841 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      901 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      961 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     1021 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     1081 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     1141 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     1201 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     1261 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1321 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1381 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1441 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     1501 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     1561 atgatttctg gaattcgcgg ccgcttctag agactagtgg gtctcaaatg agactcaagg
     1621 tcatgatgga cgtgaacaaa aaaacgaaaa ttcgccaccg aaacgagcta aatcacaccc
     1681 tggctcaact tcctttgccc gcaaagcgag tgatgtatat ggcgcttgct cccattgata
     1741 gcaaagaacc tcttgaacga gggcgagttt tcaaaattag ggctgaagat cttgcagcgc
     1801 tcgccaaaat caccccatcg cttgcttatc gacaattaaa agagggtggt aaattacttg
     1861 gtgccagcaa aatttcgcta agaggggatg atatcattgc tttagctaaa gagcttaacc
     1921 tgccctttac tgctaaaaac tcccctgaag agttagatct taacattatt gagtggatag
     1981 cttattcaaa tgatgaagga tacttgtctt taaaattcac cagaaccata gaaccatata
     2041 tctctagcct tattgggaaa aaaaataaat tcacaacgca attgttaacg gcaagcttac
     2101 gcttaagtag ccagtattca tcttctcttt atcaacttat caggaagcat tactctaatt
     2161 ttaagaagaa aaattatttt attatttccg ttgatgagtt aaaggaagag ttaatagctt
     2221 atacttttga taaagatgga aatattgagt acaaataccc tgactttcct atttttaaaa
     2281 gggatgtgtt aaataaagcc attgctgaaa ttaaaaagaa aacagaaata tcgtttgttg
     2341 gcttcactgt tcatgaaaaa gaaggaagaa aaattagtaa gctgaagttc gaatttgtcg
     2401 ttgatgaaga tgaattttct ggcgataaag atgatgaagc tttttttatg aatttatctg
     2461 aagctgatgc agcttttctc aaggtatttg atgaaaccgt acctcccaaa aaagctaagg
     2521 ggtgaaggta gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct
     2581 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2641 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2701 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     2761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3001 cgttcagccc gaccgctgcg cct
//
