LOCUS Exported 2828 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli).. ACCESSION . VERSION . KEYWORDS C84m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2828) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2828) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers misc_feature 5..724 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 5..724 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="sigma28" misc_feature 729..2828 /ApEinfo_revcolor="#6ecff6" /ApEinfo_fwdcolor="#6ecff6" /label="DVLA" misc_feature complement(730..735) /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" primer_bind complement(812..832) /Sequence="TTACCGCCTTTGAGTGAGCTG" /Hairpin_Tm="None" /Tm="60.6" /Self_Dimer_Tm="None" /%GC="52.4" /created_by="primer3" /label="SC190/Part.Seq.REV" primer_bind 2724..2744 /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /created_by="primer3" /label="SC189/Part.Seq.FOR" misc_feature 2822..2827 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" misc_feature 2822..2827 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" source 1..2828 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 736..756 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(762..779) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(933..1521) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1013..1032) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1692..2552) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2315..2334 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2553..2657) /gene="bla" /label=AmpR promoter primer_bind 2725..2743 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2747..2790 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2793..2814 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatgatgaat tcactctata ccgctgaagg tgtaatggat aaacactcgc tgtggcagcg 61 ttatgtcccg ctggtgcgtc acgaagcatt gcgcctgcag gttcgactgc ccgcgagcgt 121 ggaacttgac gatctgctac aggcgggcgg cattgggtta cttaatgccg tcgaacgcta 181 tgacgcccta caaggaacgg catttacaac ttacgcagtg cagcgtatcc gtggcgctat 241 gctggatgaa cttcgcagcc gtgactgggt gccgcgcagc gtgcgacgca acgcgcgtga 301 agtggcacag gcaatagggc aactggagca ggaacttggc cgcaacgcca cggaaactga 361 ggtagcggaa cgtttaggga tcgatattgc cgattatcgc caaatgttgc tcgacaccaa 421 taacagccag ctcttctcct acgatgagtg gcgcgaagag cacggcgata gcatcgaact 481 ggttactgat gatcatcagc gagaaaaccc gctacaacaa ctactggaca gtaatctgcg 541 ccagcgggtg atggaagcca tcgaaacgtt gccggagcgc gaaaaactgg tattaaccct 601 ctattaccag gaagagctga atctcaaaga gattggcgcg gtgctggagg tcggggaatc 661 gcgggtcagt cagttacaca gccaggctat taaacggtta cgcactaaac tgggtaagtt 721 ataaaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg 781 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 841 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 901 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag 961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 1021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 1081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 1141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 1201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 1261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 1321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 1381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 1441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 1501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 1561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 1621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 1681 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 1741 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 1801 ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta 1861 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 1921 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 1981 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 2041 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 2101 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 2161 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 2221 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 2281 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 2341 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 2401 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 2461 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 2521 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 2581 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 2641 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 2701 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa 2761 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagt 2821 gggtctca //