LOCUS       Exported                2381 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly CD part (phage lysis protein; lethal to 
            host when expressed)..
ACCESSION   .
VERSION     .
KEYWORDS    C86m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2381)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    2362..2080
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /label="DVLA"
     misc_feature    2362..2080
                     /Source="referenceToDocument: urn:local:.:an-9u0npf3"
                     /label="CD hifi backbone (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     complement(64..84)
                     /Sequence="TTACCGCCTTTGAGTGAGCTG"
                     /Hairpin_Tm="None"
                     /Tm="60.6"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="SC190/Part.Seq.REV"
     primer_bind     1976..1996
                     /Sequence="AAATAGGCGTATCACGAGGCA"
                     /Hairpin_Tm="46.5"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="SC189/Part.Seq.FOR"
     misc_feature    2058..2381
                     /Source="referenceToDocument: urn:local:.:ao-9u0npf3"
                     /label="pZA35\X174\(+Lux PCR Product (concatenated
                     sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    2058..2081
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        2058..2080
                     /label="Ligation"
     misc_feature    complement(2058..2080)
                     /modified_by="anandhswaminathan"
                     /Mismatches=0
                     /%GC="47.8"
                     /Hairpin_Tm="54.8"
                     /Self_Dimer_Tm="19.3"
                     /Pair_Dimer_Tm="16.9"
                     /Tm="57.9"
                     /annotation_group="3051639069842364f3d5e63d-1bec-4b28-bc71
                     -6bcc90eaf33a"
                     /Sequence="TGAGACCCACTAGTCTCTAGAAG"
                     /created_by="primer3"
                     /Product_Size=2100
                     /label="dva_R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2074..2079
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     CDS             2082..2354
                     /created_by="anandhswaminathan"
                     /modified_by="anandhswaminathan"
                     /label="phiX174E"
     misc_feature    complement(2330..2354)
                     /Mismatches=0
                     /Extension="GCCGCTACTAGTAGGTCTCTACCTTTA"
                     /%GC="48.0"
                     /Hairpin_Tm="44.6"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Tm="63.1"
                     /annotation_group="32161958834085719950efc0-b385-4ba0-b8a1
                     -b53c3d99a2e7"
                     /Sequence="CTCCTTCCGCACGTAATTTTTGACG"
                     /created_by="primer3"
                     /Product_Size=273
                     /label="X174E_R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2355..2381
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        2362..2381
                     /label="Ligation"
     misc_feature    complement(2363..2368)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2363..2368)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     source          1..2381
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(14..31)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(185..773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(265..284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(944..1804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1567..1586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1805..1909)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1977..1995
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1999..2042
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2045..2066
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2369..8
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
       61 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      181 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1081 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1921 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     1981 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
     2041 tctggaattc gcggccgctt ctagagacta gtgggtctca aatggtacgc tggactttgt
     2101 gggataccct cgctttcctg ctcctgttga gtttattgct gccgtcattg cttattatgt
     2161 tcatcccgtc aacattcaaa cggcctgtct catcatggaa ggcgctgaat ttacggaaaa
     2221 cattattaat ggcgtcgagc gtccggttaa agccgctgaa ttgttcgcgt ttaccttgcg
     2281 tgtacgcgca ggaaacactg acgttcttac tgacgcagaa gaaaacgtgc gtcaaaaatt
     2341 acgtgcggaa ggagtaaagg tagagaccta ctagtagcgg c
//
