LOCUS Exported 2822 bp ds-DNA circular SYN 04-DEC-2018 DEFINITION MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab). ACCESSION . VERSION . KEYWORDS C91m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2822) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2822) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 4, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers misc_feature 2803..2080 /Source="referenceToDocument: urn:local:.:e4-9u0ntrl" /label="CD hifi backbone (concatenated sequence 1)" /note="Geneious type: Concatenated sequence" CDS 2082..2795 /created_by="User" /modified_by="User" /label="sfCFP-no tag,orig seq" CDS 2082..2795 /created_by="User" /modified_by="User" /label="sfCFP-no tag,orig seq" source 1..2822 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(14..31) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(185..773) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(265..284) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(944..1804) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1567..1586 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1805..1909) /gene="bla" /label=AmpR promoter primer_bind 1977..1995 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1999..2042 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2045..2066 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 2810..8 /label=BioBrick suffix /note="universal suffix for all parts" ORIGIN 1 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 61 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 181 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1081 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 1261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 1321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 1381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 1441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 1501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 1561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 1621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 1681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 1741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 1801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 1861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 1921 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 1981 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 2041 tctggaattc gcggccgctt ctagagacta gtgggtctca aatgcgtaaa ggcgaagagc 2101 tgttcactgg tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt 2161 tttccgtgcg tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca 2221 tctgtactac tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttggg 2281 gtgttcagtg ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg 2341 ccatgccgga aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca 2401 aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag 2461 gcattgactt taaagaagac ggcaatatcc tgggccataa gctggaatac aattacatca 2521 gcgacaatgt ttacatcacc gccgataaac aaaaaaatgg cattaaagcg aattttaaaa 2581 ttcgccacaa cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc 2641 caatcggtga tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc 2701 tgtctaaaga tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag 2761 cgggcatcac gcatggtatg gatgaactgt acaaataaag gtagagacct actagtagcg 2821 gc //