LOCUS       Exported                4757 bp ds-DNA     circular SYN 05-DEC-2018
DEFINITION  MoClo golden gate assembly CD part for T7 RNA polymerase (use with 
            P18m)..
ACCESSION   .
VERSION     .
KEYWORDS    C95m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4757)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    3374..3394
                     /Color="#a6ccff"
                     /Transferred_From="BioBrick_suffix"
                     /Transferred_Similarity="100.00%"
                     /label="BioBrick suffix"
     rep_origin      complement(3542..4159)
                     /direction="RIGHT"
                     /note="Medium copy ColE1 origin of  replication; color:
                     #ffff00"
                     /X-Transferred_From="pUC High Copy Cole1 ori"
                     /X-Transferred_Similarity="99.84%"
                     /modified_by="Andrey"
                     /Transferred_From="Cole1 Medium Copy"
                     /Transferred_Similarity="100.00%"
                     /label="Cole1 Medium Copy"
     promoter        3977..4006
                     /Color="#ffffff"
                     /Transferred_From="RNA_I_promoter"
                     /Transferred_Similarity="100.00%"
                     /label="RNA I promoter"
     promoter        complement(4131..4159)
                     /Color="#ffffff"
                     /Transferred_From="RNAII_promoter"
                     /Transferred_Similarity="100.00%"
                     /label="RNAII promoter"
     source          1..4757
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     196..215
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(434..538)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     606..624
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      628..671
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    674..695
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    3374..3394
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(3400..3417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3571..4159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3651..3670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4330..433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
       61 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
      121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      541 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
      601 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
      661 aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctcaa atgaacacga
      721 ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg ttcaacactc
      781 tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag catgagtctt
      841 acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg
      901 ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag atgattgcac
      961 gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg acagccttcc
     1021 agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag accactctgg
     1081 cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca atcggtcggg
     1141 ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag cacttcaaga
     1201 aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa gcatttatgc
     1261 aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg tggtcttcgt
     1321 ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc attgagtcaa
     1381 ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac tctgagacta
     1441 tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg ctggctggca
     1501 tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc attactggtg
     1561 gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac agtaagaaag
     1621 cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt aacattgcgc
     1681 aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta atcaccaagt
     1741 ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc ccgatgaaac
     1801 cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct gccgctgctg
     1861 tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc atgcttgagc
     1921 aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg gactggcgcg
     1981 gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc aaaggactgc
     2041 ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg aaaatccacg
     2101 gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag ttcattgagg
     2161 aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact tggtgggctg
     2221 agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg gtacagcacc
     2281 acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc tctggcatcc
     2341 agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac ttgcttccta
     2401 gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag attctacaag
     2461 cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag aacactggtg
     2521 aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg ctggcttacg
     2581 gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg tccaaagagt
     2641 tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat tccggcaagg
     2701 gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg atttgggaat
     2761 ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag tctgctgcta
     2821 agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc aagcgttgcg
     2881 ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag aagcctattc
     2941 agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc attaacacca
     3001 acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct aactttgtac
     3061 acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag aagtacggaa
     3121 tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac gctgcgaacc
     3181 tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat gtactggctg
     3241 atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa atgccagcac
     3301 ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc gcgttcgcgt
     3361 aaaggtagag acctactagt agcggccgct gcaggcttcc tcgctcactg actcgctgcg
     3421 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3481 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3541 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     3601 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3661 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3721 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3781 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3841 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3901 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3961 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4021 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4081 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4141 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4201 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4261 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4321 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4381 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4441 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
     4501 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4561 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4621 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4681 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4741 caaaaaagcg gttagct
//
