LOCUS Exported 2846 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).. ACCESSION . VERSION . KEYWORDS C103m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2846) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2846) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers misc_feature <1..>42 /Source="referenceToDocument: urn:local:.:df-9u0ntrc" /label="C20m PCR Product LAA only (concatenated sequence 2)" /note="Geneious type: Concatenated sequence" misc_feature 1..42 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 1..42 /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="#ff8040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="LAA" CDS <1..42 /codon_start=1 /created_by="User" /label="sfYFP ssrA" misc_feature complement(48..53) /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" source 1..2846 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 54..74 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(80..97) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(251..839) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(331..350) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1010..1870) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1633..1652 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1871..1975) /gene="bla" /label=AmpR promoter primer_bind 2043..2061 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2065..2108 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2111..2132 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 actagtgcag cgaacgacga aaattacgcc cttgcagcgt gaaggtagag acctactagt 61 agcggccgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 241 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 421 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 661 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 1021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 1081 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 1141 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 1201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 1261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 1321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 1381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 1441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 1501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 1561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 1621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 1681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 1741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 1801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 1861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 1921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 1981 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 2041 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg 2101 atgatttctg gaattcgcgg ccgcttctag agactagtgg gtctcaaatg atggtcagta 2161 aaggcgaagc agttatcaaa gagttcatgc gcttcaaagt tcatatggaa gggtcgatga 2221 acgggcacga atttgaaatt gaaggcgaag gcgaaggccg cccatatgaa gggacccaaa 2281 ccgcaaagct taaggttact aaaggcggtc cattaccctt ttcgtgggac attttaagcc 2341 cacagtttat gtacgggagt cgcgctttca tcaagcaccc tgcggacatc ccagattact 2401 acaaacagtc tttccccgag gggttcaagt gggagcgcgt gatgaacttc gaggatggcg 2461 gagccgtgac ggtcacccaa gatacctctt tggaggacgg tacgttgatc tacaaagtga 2521 aattgcgtgg cacgaatttt ccacctgatg ggcctgtcat gcagaaaaag acaatgggat 2581 gggaagcttc cacggagcgc ctttacccag aggacggtgt tcttaaaggg gatatcaaaa 2641 tggcgctgcg tcttaaagat ggaggccgct acctggcgga cttcaagact acttacaagg 2701 ccaaaaaacc agtgcagatg ccgggtgcgt acaatgtaga tcgtaaatta gatattacaa 2761 gtcacaatga agattacacg gtcgtagagc agtatgagcg cagtgagggg cgtcactcta 2821 cgggcggtat ggacgagtta tacaag //