LOCUS Exported 2142 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly BC part for UTR1 (very strong RBS).. ACCESSION . VERSION . KEYWORDS U4m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2142) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2142) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..2142 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 5..37 /note="UTR1" /label="UTR1" RBS 12..32 /Color="#a6acb3" /Transferred_From="RBS" /Transferred_Similarity="100.00%" /label="RBS" ORF complement(22..>38) /Genetic_code="Standard" /Reading_frame=3 /created_by="ORF Finder" /label="ORF 9 (frame 3)" misc_feature 43..2142 /ApEinfo_revcolor="#6ecff6" /ApEinfo_fwdcolor="#6ecff6" /label="DVLA" misc_feature complement(44..49) /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" misc_feature 50..70 /Color="#a6ccff" /Transferred_From="BioBrick_suffix" /Transferred_Similarity="100.00%" /label="BioBrick suffix" primer_bind complement(126..146) /Sequence="TTACCGCCTTTGAGTGAGCTG" /Hairpin_Tm="None" /Tm="60.6" /Self_Dimer_Tm="None" /%GC="52.4" /created_by="primer3" /label="SC190/Part.Seq.REV" primer_bind 2038..2058 /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /created_by="primer3" /label="SC189/Part.Seq.FOR" misc_feature 2107..2128 /Color="#99ccff" /Transferred_From="BioBrick_prefix_(2)" /Transferred_Similarity="100.00%" /label="BioBrick prefix" misc_feature 2136..2141 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" misc_feature 2136..2141 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" source 1..2142 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 11..33 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" misc_feature 50..70 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(76..93) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(247..835) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(327..346) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1006..1866) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1629..1648 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1867..1971) /gene="bla" /label=AmpR promoter primer_bind 2039..2057 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2061..2104 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2107..2128 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 tactaataat tttgtttaac tttaagaagg agatatacaa tgagagacct actagtagcg 61 gccgctgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 241 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 661 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 901 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 961 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 1021 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 1081 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 1141 accgcgcgac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 1201 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 1261 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 1321 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 1381 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 1441 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 1501 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 1561 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 1621 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 1681 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 1741 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 1801 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 1861 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 1921 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 1981 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 2041 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga 2101 tttctggaat tcgcggccgc ttctagagac tagtgggtct ca //