LOCUS Exported 2165 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515).. ACCESSION . VERSION . KEYWORDS T13m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2165) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2165) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers terminator 5..61 /created_by="anandhswaminathan" /modified_by="anandhswaminathan" /label="ECK120033737 (2nd strongest Voigt natural, 313x)" misc_feature 66..2165 /ApEinfo_revcolor="#6ecff6" /ApEinfo_fwdcolor="#6ecff6" /label="DVLA" misc_feature complement(67..72) /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" misc_feature 74..2151 /ApEinfo_revcolor="#6ecff6" /ApEinfo_fwdcolor="#6ecff6" /Transferred_From="DVA" /Transferred_Similarity="100.00%" /label="DVLA" primer_bind complement(149..169) /Sequence="TTACCGCCTTTGAGTGAGCTG" /Hairpin_Tm="None" /Tm="60.6" /Self_Dimer_Tm="None" /%GC="52.4" /created_by="primer3" /Transferred_From="DVA" /Transferred_Similarity="100.00%" /label="SC190/Part.Seq.REV" primer_bind 2061..2081 /Sequence="AAATAGGCGTATCACGAGGCA" /Hairpin_Tm="46.5" /Tm="59.6" /Self_Dimer_Tm="None" /%GC="47.6" /created_by="primer3" /Transferred_From="DVA" /Transferred_Similarity="100.00%" /label="SC189/Part.Seq.FOR" misc_feature 2159..2164 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" misc_feature 2159..2164 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" source 1..2165 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 73..93 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(99..116) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(270..858) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(350..369) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1029..1889) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1652..1671 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1890..1994) /gene="bla" /label=AmpR promoter primer_bind 2062..2080 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2084..2127 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2130..2151 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aggtggaaac acagaaaaaa gcccgcacct gacagtgcgg gctttttttt tcgaccaaag 61 ggcttagaga cctactagta gcggccgctg caggcttcct cgctcactga ctcgctgcgc 121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 241 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat 301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 1021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 1081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 1141 tctggcccca gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca 1201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 1261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 1321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 1381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 1441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 1501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 1561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 1621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 1681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 1741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 1801 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 1861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 1921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 1981 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 2041 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa 2101 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga gactagtggg 2161 tctca //