LOCUS Exported 2155 bp ds-DNA circular SYN 05-DEC-2018 DEFINITION MoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).. ACCESSION . VERSION . KEYWORDS T15m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2155) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2155) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 5, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers terminator 5..51 /created_by="Andrey" /X-Transferred_From="L3S3P11" /X-Transferred_Similarity="100.00%" /Color="#ffffff" /modified_by="Andrey" /Transferred_From="fivetermg" /Transferred_Similarity="100.00%" /label="L3S3P11 mod (172.6?)" misc_feature 56..2155 /Color="#cc99ff" /Transferred_From="CIDAR_DVA_backbone" /Transferred_Similarity="100.00%" /label="CIDAR DVA backbone" misc_feature 63..83 /Color="#a6ccff" /Transferred_From="BioBrick_suffix" /Transferred_Similarity="100.00%" /label="BioBrick suffix" primer_bind complement(139..159) /Sequence="TTACCGCCTTTGAGTGAGCTG" /Hairpin_Tm="None" /Tm="60.6" /Self_Dimer_Tm="None" /%GC="52.4" /created_by="primer3" /modified_by="andrey" /Transferred_From="Part.Seq.REV" /Transferred_Similarity="100.00%" /label="Part.Seq.REV" terminator complement(2074..2117) /Color="#ffffff" /Transferred_From="bacterial_terminator" /Transferred_Similarity="100.00%" /label="bacterial terminator" misc_feature 2120..2139 /Color="#99ccff" /Transferred_From="BioBrick_prefix" /Transferred_Similarity="100.00%" /label="BioBrick prefix" misc_feature 2120..2139 /Color="#99ccff" /Transferred_From="BioBrick_prefix" /Transferred_Similarity="100.00%" /label="BioBrick prefix" source 1..2155 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 63..83 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(89..106) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(260..848) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(340..359) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1019..1879) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1642..1661 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1880..1984) /gene="bla" /label=AmpR promoter primer_bind 2052..2070 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2074..2117 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2120..2141 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aggtccaatt attgaacacc cttcggggtg tttttttgtt tctggtctgc cgcttagaga 61 cctactagta gcggccgctg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc 121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 241 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc 301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1141 gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca gcaataaacc 1201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1321 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 1381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 1441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 1501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 1561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 1621 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 1681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 1741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 1801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 1861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 1921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 1981 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 2041 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 2101 tcgctaagga tgatttctgg aattcgcggc cgcttctaga gactagtggg tctca //