LOCUS pJW1592__GFP_SEC_B 10758 bp ds-DNA circular 29-JUN-2016 DEFINITION Cloning vector pUC19, complete sequence. KEYWORDS . SOURCE Cloning vector pUC19 ORGANISM Cloning vector pUC19 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2686) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (18-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT To prepare vector for cloning: -Digest with ClaI and SpeI to fuse 5' homology arm directly to mNG, or -Digest with AvrII and SpeI to include a flexible linker (for C-terminal tags). COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 404..1010 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=This sequence will be replaced by the homology arm /ApEinfo_fwdcolor=#cc9966 /ApEinfo_revcolor=green CDS join(2767..2907,2955..3788) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=SQT-1 /ApEinfo_fwdcolor=#00cccc /ApEinfo_revcolor=#006666 CDS 6097..7122 /note="hygromycinR" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=hygR /ApEinfo_fwdcolor=#006666 /ApEinfo_revcolor=#006666 misc_feature 7540..7551 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=spacer /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=green misc_feature 1983..2012 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=myc /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=green protein_bind 7552..7556 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=Flag /ApEinfo_fwdcolor=#cc3366 /ApEinfo_revcolor=green misc_RNA complement(8939..9491) /note="RNAII transcript (complementary strand)" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=RNAII transcript (complementary strand) /ApEinfo_fwdcolor=#cccc33 /ApEinfo_revcolor=#999933 misc_RNA 9381..9488 /note="RNAI transcript" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=RNAI transcript /ApEinfo_fwdcolor=#cccc33 /ApEinfo_revcolor=#999933 CDS complement(join(4161..4402,4454..4824,4876..5291)) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=Cre /ApEinfo_fwdcolor=#6699cc /ApEinfo_revcolor=#006666 misc_feature 7558..7575 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=GSGGGGlinker (GS is a BamHI site) /ApEinfo_fwdcolor=#c6b1ff /ApEinfo_revcolor=green rep_origin complement(8939..9527) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=pMB1 origin of replication (counter-clockwise) (RN /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink protein_bind 7888..7914 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=Flag(1) /ApEinfo_label=Flag /ApEinfo_fwdcolor=#ff6699 /ApEinfo_revcolor=green misc_feature 7564..7575 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=GSGGGGlinker (GS is a BamHI site)(1) /ApEinfo_label=GSGGGGlinker (GS is a BamHI site) /ApEinfo_fwdcolor=#c6b1ff /ApEinfo_revcolor=green -35_signal 9345..9350 /note="RNAI promoter (clockwise); TTGAAG" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=RNAI promoter (clockwise); TTGAAG /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink protein_bind 7861..7887 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=Flag(2) /ApEinfo_label=Flag /ApEinfo_fwdcolor=#ff3366 /ApEinfo_revcolor=green -35_signal complement(9522..9527) /note="RNAII promoter (counter-clockwise); TTGAGA" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=RNAII promoter (counter-clockwise); TTGAGA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 7672..7806 /label=AID* (from Dernburg lab) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green protein_bind 7834..7860 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=Flag(3) /ApEinfo_label=Flag /ApEinfo_fwdcolor=#cc3366 /ApEinfo_revcolor=green primer_bind 378..395 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green intron 1929..1939 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=intron /ApEinfo_fwdcolor=#cc66ff /ApEinfo_revcolor=#ff00ff -10_signal 9367..9372 /note="RNAI promoter (clockwise); GCTACA" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=RNAI promoter (clockwise); GCTACA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 7576..7644 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=BioTag /ApEinfo_fwdcolor=#ffdf0d /ApEinfo_revcolor=green misc_feature 1940..1973 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=loxP /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 -10_signal complement(9501..9506) /note="RNAII promoter (counter-clockwise); CGTAAT" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=RNAII promoter (counter-clockwise); CGTAAT /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS complement(9701..10360) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow misc_feature 1980..1982 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=spacer(1) /ApEinfo_label=spacer /ApEinfo_fwdcolor=#cccccc /ApEinfo_revcolor=green misc_difference 3018..3018 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=e1350 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 CDS complement(9698..10558) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=beta-lactamase /ApEinfo_fwdcolor=#00cccc /ApEinfo_revcolor=#006666 terminator 2016..2396 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=let-858 Terminator /ApEinfo_fwdcolor=#cc0000 /ApEinfo_revcolor=#cc0000 intron 1974..1979 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=intron(1) /ApEinfo_label=intron /ApEinfo_fwdcolor=#cc66ff /ApEinfo_revcolor=#ff00ff primer_bind complement(8534..8553) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green 5'UTR join(2681..2708,2765..2766) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=sqt-1 5'UTR /ApEinfo_fwdcolor=#ccffcc /ApEinfo_revcolor=green misc_signal 1902..1922 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=TEV site /ApEinfo_fwdcolor=#ff6600 /ApEinfo_revcolor=#ff6600 CDS join(1038..1340,1389..1463,1515..1634,1686..1901) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=GFP /ApEinfo_fwdcolor=#00ff33 /ApEinfo_revcolor=#006666 misc_feature 661..966 /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ccdB /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin complement(8921..9603) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 3'UTR 3789..3825 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=sqt-1 3'UTR /ApEinfo_fwdcolor=#cc3333 /ApEinfo_revcolor=#cc3333 promoter complement(5292..5691) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=hsp16-41 promoter /ApEinfo_fwdcolor=#0080c0 /ApEinfo_revcolor=#0080c0 promoter 2397..2766 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=Psqt1 /ApEinfo_fwdcolor=#33cc00 /ApEinfo_revcolor=#33cc00 CDS 754..963 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ccdB(1) /ApEinfo_label=ccdB /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow 3'UTR complement(3826..4157) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=tbb-2 3'UTR /ApEinfo_fwdcolor=#cc3333 /ApEinfo_revcolor=#993333 intron 7534..7539 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=intron(2) /ApEinfo_label=intron /ApEinfo_fwdcolor=#cc66ff /ApEinfo_revcolor=#ff00ff 3'UTR 7148..7499 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=unc-54 3'UTR /ApEinfo_fwdcolor=#990000 /ApEinfo_revcolor=#cc0000 misc_feature 7500..7533 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=loxP(1) /ApEinfo_label=loxP /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 promoter 5692..6054 /note="Prps-0" /note="/ApEinfo_fwdcolor=pink" /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink; /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=Prps-0 /ApEinfo_fwdcolor=#339933 /ApEinfo_revcolor=#339933 misc_feature 8523..8529 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=This sequence will be deleted if ClaI is used /ApEinfo_fwdcolor=#ffcc99 /ApEinfo_revcolor=green misc_feature join(7915..7922,7924..8522) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=This sequence will be replaced by the homology arm(1) /ApEinfo_label=This sequence will be replaced by the homology arm /ApEinfo_fwdcolor=#cc9966 /ApEinfo_revcolor=green misc_feature complement(7960..8265) /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ccdB(2) /ApEinfo_label=ccdB /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS complement(7963..8172) /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ccdB(3) /ApEinfo_label=ccdB /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow misc_structure 1011..1037 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=Flexible Linker (will be deleted if ClaI is used) /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=green misc_feature 7176..7198 /label=seq oligo 2976 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT 421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT 481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA 541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG 601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA 661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA 721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT 781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC 841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG 901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA 961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG 1021 GAGCCTCAGG AGCATCGATG AGTAAAGGAG AAGAATTGTT CACTGGAGTT GTCCCAATCC 1081 TCGTCGAGCT CGACGGAGAC GTCAACGGAC ACAAGTTCTC CGTCTCCGGA GAGGGAGAGG 1141 GAGACGCCAC CTACGGAAAG CTCACCCTCA AGTTCATCTG CACCACCGGA AAGCTCCCAG 1201 TCCCATGGCC AACCCTCGTC ACCACCTTCT GCTACGGAGT CCAATGCTTC TCCCGTTACC 1261 CAGACCACAT GAAGCGTCAC GACTTCTTCA AGTCCGCCAT GCCAGAGGGA TACGTCCAAG 1321 AGCGTACCAT CTTCTTCAAG gtaagtttaa acatatatat actaactact gattatttaa 1381 attttcagGA CGACGGAAAC TACAAGACCC GTGCCGAGGT CAAGTTCGAG GGAGACACCC 1441 TCGTCAACCG TATCGAGCTC AAGgtaagtt taaacagttc ggtactaact aaccatacat 1501 atttaaattt tcagGGAATC GACTTCAAGG AGGACGGAAA CATCCTCGGA CACAAGCTCG 1561 AGTACAACTA CAACTCCCAC AACGTCTACA TCATGGCCGA CAAGCAAAAG AACGGAATCA 1621 AGGTCAACTT CAAGgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt 1681 ttcagATCCG TCACAACATC GAGGACGGAT CCGTCCAACT CGCCGACCAC TACCAACAAA 1741 ACACCCCAAT CGGAGACGGA CCAGTCCTCC TCCCAGACAA CCACTACCTC TCCACCCAAT 1801 CCGCCCTCTC CAAGGACCCA AACGAGAAGC GTGACCACAT GGTCCTCCTC GAGTTCGTCA 1861 CCGCCGCCGG AATCACCCAC GGAATGGACG AGCTCTACAA GGAGAATCTG TACTTTCAAT 1921 CCGGAAAGgt aagtttaaaA TAACTTCGTA TAGCATACAT TATACGAAGT TATtttcagG 1981 GAGAGCAAAA GCTTATCTCC GAGGAGGACC TCTAAGGATG ATCGACGCCa ACGTCGTTGA 2041 ATTTTCAAAT TTTAAATACT GAATATTTGT TTTTTTTCCT ATTATTTATT TATTCTCTTT 2101 GTGTTTTTTT TCTTGCTTTC TAAAAAATTA ATTCAATCCA AATCTAAaca tttttttttc 2161 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat 2221 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca 2281 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa 2341 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aactagctgc 2401 cctcgattgt ctatcttcca ttctcaccaa gtttcaacaa ctttattcag tcattcttct 2461 ctggttcatt tcccaaccaa tcttcccgaa taaagcccat ctgtcctttc ggccacattc 2521 ctcacctaca tccgtactcc ttatctcccg ttcttatcca gtcttttatc gtctctgcgt 2581 ctcgcacact gtctcgtcgg taatgtaaaa aaaaaacaaa aaaaacttga aaacttgcgg 2641 aaagatttcc taataaaagg agacgaatcc ggtaggaaat cgacaagtct ctgcttctcg 2701 aagactaggt gagttacatt tctttggccg aattattttg aaatcctaaa tttgataatt 2761 tcagagATGT CTGTAAAACT TGCGTGTTAT GTGACGGCTT CGGTCACTGT CGCCACTCTT 2821 ATGGTATGCT TCATGACCAT GTCAACCATC TACTCGGAGG TTGATGGATT CAGAGAGAAG 2881 CTTGATACCG AGATGAATGT GTTCAGAgtg agttaaatta tattttgaat tttaaataat 2941 ttttaatttt ctagCAATCT ACCAATGGAT TGTGGAAGGA CATAGTTGTC ATCGGAAGAT 3001 CTAGCAAGCG TGTCCGTTGT CAATATGAAG AGACCAACGC TACCCCAACT CCACATGCTG 3061 ATGGATCCCC ATCTGCTCCA CCAGGTCAAC CACCAGCAGT TCCACCAGTC TTCAACCAGC 3121 CAAAGACTCC AAATGGAGCC AATGGAAATG GACCAACCTG CAACTGCAAT GCTGATAACA 3181 AGTGCCCAGC TGGACCATCC GGACCAAAGG GAGTTCCAGG AGTTCCAGGA CTCGACGGAG 3241 TTCCAGGACT TGACGGTGTT CCAGGAGTTG GAGCTGATGA TATCGCTCCA CAACGCGAGT 3301 CTGTCGGATG CTTCACTTGC CCACAAGGAC CAGTTGGACC ACCAGGAGCT CTTGGAAGAC 3361 CAGGACCACG TGGACTTCCA GGACCAAGAG GACAAAATGG AAACCCAGGA AGAGATGGAC 3421 AACCAGGACA TCCAGGAGAG CAAGGATCAT CCGGTCAAAT CGGAAAGATC GGAGAGCCAG 3481 GACCACCAGG AGAGAAGGGA CGCGACGCCG AGCATCCAAT CGGAAGACCA GGACCAAAGG 3541 GACCAAGAGG AGATCAAGGA CCAACAGGAC CAGCTGGACA GAACGGTCTT CACGGACCAC 3601 CAGGAGAGCC AGGAACCGTT GGACCAGAAG GACCATCTGG AAAGCAAGGA CGTCAAGGAC 3661 CAGACGGAAC CCAGGGAGAG ACTGGACCAG ACGGAAGACC AGGAAAGGAT GCCGAGTACT 3721 GCCAGTGCCC AGACAAGTCT CCACCATCAG AGGCTGTCAA CGCCAACCGT GGATACAGAA 3781 ATATCTAAat tgttggtgtt ttctaataaa atatttgaga ttcagtgaga cttttttctt 3841 ggcggcacaa taaagtttcc tctttttgtt gggtcactca acgaacacaa tacaaatatt 3901 tgagcgctcg catttttctc aacctttcgg gtttaaagca cttcaaaatg gaattgaaga 3961 gagattgtga aagtacatca gacagaatca cattttgata gcattcactt cactcagatg 4021 caaacttttc agtgactata aaaagtcata aaataataac attaaaaaag caaataaatt 4081 agcgagagaa ttttttgaca aaaagaaaga agagtgatag agaagaaggg aatgcttgaa 4141 aggattttgc atttatctta ATCACCGTCT TCGAGAAGAC GAACCATGGC GCCAGTTTCA 4201 GAATCCAAGT TTCTAATATA GTTCATCACA ATGTTGACGT TGGTCCAACC TCCGGCTTGC 4261 ATAATCTCTG GGATGGAAAC TCCAGCGCGA GCCATGTCAC GGGCAGCTCC GACACGGGCG 4321 GAATGTCCAG ACCAAGCCAA GTAACGTTGT CCGGAATCAT CCTTGGCTCC GTAGATGAGA 4381 CGGTGGGTAG CTTCGAAGAT TCctgaaaat ttattttcag cttattctta gctaatatgt 4441 tgtttaaact tacCTTCAAG AGCTCGAGTC GAGAGTTGTG ATGTTGCACT CGGGGCAGCA 4501 ACTCCGTTCT TGCGAACACG ACAGAACAGG TAGTTGTTTG GATCATCGGC GACTCCGGAA 4561 ACCGAAATCC AGCGCTCAAC GAGCTTGGTA ACTCCAAGGG AGAGCGCTTT TTCGACTCCG 4621 GCAGTCGAGA CCAGCGTTTT TGTACGTCCG ATGTGGATGA GCATTCTTCC TCCGTCTGTG 4681 CGACTAATAT CCTTGACGCG AATGCGGGCA ATTTCGGCGA TACGGAGAAG TGTGTTGTAA 4741 GCAATTCCGA GGAAGGCGAG GTTTCGGATG TCCTGGCAAC GATCGGAATT TTCCATAAGA 4801 GAACGGACCT GATCGAAGTC GGTTctgaaa atttcgtcaa gtcttaggat tgaatacatt 4861 aatatttaaa cttacCTTTC GAAGGCGAGG GCTTGCTTGG CTCTCTCTCC AGCATCAACA 4921 TTTTCCTTGC GGATACGTCT CATTACGAGA GATACGGCAT TAGAATCTGA TGGGCGTGGA 4981 AGTCCAGATC GGCGATGGAG CATATTGAGC TGTCCGAGAT GTTGTTGGAT CGTCTTCACC 5041 GCCAATCCAC GAGCTTGGAG GTAGAGCAAG TAGTCACGAA CGTCTTCTGG CTCGGCTGGG 5101 AACCACTTGC GGTTGTTAAG CTTGCACCAG GCAGCCCAAG AACGGCACAC TGAGAGAAGC 5161 ATCTTCCAGG TGTGCTCGGA GAAGGCTTGT CTGTCACGGA ACATATCCAT GAGGTTCTTG 5221 CGAACTTCAT CACTTGTCGC GTCAACTGGA AGTGCTGGAA GATTTTGATG AACAGTGAGA 5281 AGATTTGACA Tattttcgaa gttttttaga tgcactagaa caaagcgtgt tggcttcctc 5341 tgagcccgct ttccttatat acccgcattc tgcagcctta cagaatgttc tagaaggtcc 5401 tagatgcatt cgtttgaaaa tactcccggt gggtgcaaag agacgcagac ggaaaatgta 5461 tctgggtctc tttattgtgt acactacttt tccatgtacc gaatgtgagt cgccctcctt 5521 ttgcaacaag cagctcgaat gttctagaaa aaggtggaaa atagtataaa taccgttgaa 5581 aataaatacc gaacaacatt tgctctaatt gtgaaattag aaatcttcaa actataatca 5641 tgtcacttta ccactatttc cgtccagctc aacgttccgt ttttggtgat cATTTTTGCT 5701 TTCGTCGTAA ATCTACACAC GCGTCTCTTC CGTGCGAGAG TCCAAGCCAG CAGCCAAATT 5761 CGTTGACTGA GTATTCAACG TTTATACGTT GTCGGCAACG AGAAATAGGA AAATGCATCG 5821 GGAAATGTTC TTTTTTCGAT TTTTTCCAAG GTTTTGACAA ATTTTACCAC GAATTTTGCT 5881 ATGTTTTCAA TTAAAAAATA TGTTATTCAA CTGTTTCTAT GAGGAAAATA AGGCTTTGCA 5941 TGTAATTTTC TTATTCAGCA TAATTTTTAA TTAATTTGAA TTTTCTGTCC TAACGTTTAT 6001 TTTGTTTTCT TGGTTATGAC TGATCTGAAA TTAATTTTTG AATTTTAAGG TAATATGTCA 6061 GGCGGTGCCG CAAGTTTGTA CAAAAAAGCA GGCTCCATGA AAAAGCCTGA ACTCACCGCG 6121 ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC 6181 TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG ATATGTCCTG 6241 CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTTATCG GCACTTTGCA 6301 TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA GAGCCTGACC 6361 TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG 6421 CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC CGATCTTAGC 6481 CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC TACATGGCGT 6541 GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT GATGGACGAC 6601 ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC CGAGGACTGC 6661 CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA ACAATGTCCT GACGGACAAT 6721 GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGATTC CCAATACGAG 6781 GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA TGGAGCAGCA GACGCGCTAC 6841 TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC TCCGGGCGTA TATGCTCCGC 6901 ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA ATTTCGATGA TGCAGCTTGG 6961 GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGAGCCG GGACTGTCGG GCGTACACAA 7021 ATCGCCCGCA GAAGCGCGGC CGTCTGGACC GATGGCTGTG TAGAAGTACT CGCCGATAGT 7081 GGAAACCGAC GCCCCAGCAC TCGTCCGAGG GCAAAGGAAT AGACCCAGCT TTCTTGTACA 7141 AAGTGGGTCC AATTACTCTT CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC 7201 CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG 7261 TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC 7321 GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG 7381 TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA 7441 AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAA 7501 TAACTTCGTA TAGCATACAT TATACGAAGT TATtttcagG GAGCCGGATC TGATTAcGGA 7561 TCCGGAGGTG GCGGGATGGC TTCTTCTCTT CGTCAGATCC TCGACTCTCA GAAGATGGAG 7621 TGGCGTTCTA ACGCTGGAGG ATCTGGCGGA GGAGGCAGTG GTAGTGGCAG Tatgcctaaa 7681 gatccagcca aacctccggc caaggcacaa gttgtgggat ggccaccggt gagatcatac 7741 cggaagaacg tgatggtttc ctgccaaaaa tcaagcggtg gcccggaggc ggcggcgttc 7801 gtgaagGGTG GTGGCGGTTC TGGCTCAGGA TCTGATTATA AAGACGATGA CGATAAGCGT 7861 GACTACAAGG ACGACGACGA CAAGCGTGAT TACAAGGATG ACGATGACAA GAGActagtT 7921 CGaACCTGCA GACTGGCTGT GTATAAGGGA GCCTGACATT TATATTCCCC AGAACATCAG 7981 GTTAATGGCG TTTTTGATGT CATTTTCGCG GTGGCTGAGA TCAGCCACTT CTTCCCCGAT 8041 AACGGAGACC GGCACACTGG CCATATCGGT GGTCATCATG CGCCAGCTTT CATCCCCGAT 8101 ATGCACCACC GGGTAAAGTT CACGGGGGAC TTTATCTGAC AGCAGACGTG CACTGGCCAG 8161 GGGGATCACC ATCCGTCGCC CGGGCGTGTC AATAATATCA CTCTGTACAT CCACAAACAG 8221 ACGATAACGG CTCTCTCTTT TATAGGTGTA AACCTTAAAC TGCATTTCAC CAGCCCCTGT 8281 TCTCGTCGGC AAAAGAGCCG TTCATTTCAA TAAACCGGGC GACCTCAGCC ATCCCTTCCT 8341 GATTTTCCGC TTTCCAGCGT TCGGCACGCA GACGACGGGC TTCATTCTGC ATGGTTGTGC 8401 TTACCGAACC GGAGATATTG ACATCATATA TGCCTTGAGC AACTGATAGC TGTCGCTGTC 8461 AACTGTCACT GTAATACGCT GCTTCATAGC ATACCTCTTT TTGACATACT TCGGGTtcct 8521 aggaaatcga taaatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 8581 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 8641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 8701 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 8761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 8821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 8881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 8941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 9001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 9061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 9121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 9181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 9241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 9301 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 9361 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 9421 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 9481 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 9541 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 9601 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 9661 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 9721 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 9781 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 9841 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 9901 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 9961 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 10021 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 10081 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 10141 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 10201 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 10261 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 10321 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 10381 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 10441 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 10501 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 10561 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 10621 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 10681 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 10741 tcacgaggcc ctttcgtc //