LOCUS       Exported                9749 bp ds-DNA     circular SYN 06-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2018 from SnapGene Viewer 4.2.8
            http://www.snapgene.com
COMMENT     [DEFINITION]: [LOCUS]: Construct_1 [ACCESSION]: pVec1
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    940..969
                     /label=intron
     misc_feature    1099..1572
                     /label=tol2 insert
     misc_feature    1350..1445
                     /label=miR-100 hairpin
     misc_feature    1965..2657
                     /label=dendra2
     misc_feature    2637..2654
                     /label=dendra primer
     CDS             2664..2681
                     /label=6His
     misc_feature    3211..3798
                     /label=puro res
     CDS             complement(5066..5077)
                     /label=FactorXa site
     misc_feature    5282..5334
                     /label=delta U3
     LTR             5335..5515
                     /label=HIV-1 5 LTR
     misc_feature    5748..5825
                     /label=SV40 ORI
     promoter        5885..5913
                     /label=Amp prom
     CDS             6153..6812
                     /label=AmpR
     rep_origin      6964..7592
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        7898..7927
                     /label=lac
     misc_binding    7932..7954
                     /label=LacO
     primer_bind     7960..7980
                     /label=M13-rev
     primer_bind     7998..8017
                     /label=T3
     LTR             8270..8450
                     /label=HIV-1 5 LTR
     misc_feature    8561..8605
                     /label=HIV-1 psi pack
     misc_feature    9115..9348
                     /label=RRE
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat ggatgaggaa atcgctgccc tggtcgttga caacggctcc ggtatgtgca
      601 aagccggttt tgctggagat gatgcccctc gtgctgtttt cccctccatt gttggacgac
      661 ccagacatca ggtacattta ataaaccatc ctaataacat gttaatcata ttgaaccaca
      721 ttaaactctc atttgtgcat tttacatctt ttaaaaccga tttatattcg tttttagata
      781 atgaagttta ttaaagtgtt aattcacagc tttattgcct gttttgagaa tctgcttgca
      841 taagtgattt ttaaacttga tctgattctc gtttgtttcg aagttcttca gctttgaaag
      901 tcagatcttc attcacagcc atgccatagt tttaggatac agtataagta gacgaattgg
      961 gatgcatcgt agattttatg ctgtgtttgt tatggttttt cagtggtaga gattttcaga
     1021 gtattattag ggttatagtc agtgtcaggt ttatttatcg tcagcgtgat gggcaaaaaa
     1081 cagctggcag tacgggctgg aggcagcaga ggcttttact tcggacccat cagttttctt
     1141 gattgtttgt tagctgaacc gtaaaactgc aaatatgttt tgcgcctcag tgtttgagct
     1201 gccattccta tttacgtatg cagtcgcctt gctttcgcat cgcctttata tgattgaaat
     1261 tcatttccag atttagaatg atttgttttc gtcttttata acgaaagact gaaagtggat
     1321 ggatgacttg tgaacttgtt tttctccagg aagagacctg cctgctgaca caaacccgta
     1381 gatccgaact tgtggtgact gtccacacaa gcttgtatct ataggtatct gtctgtgtgg
     1441 ccttcacacc tactgatcag ggcctcgttt caaactgtcc tgtgctattc tgtataggtt
     1501 tttcaaagca agaaagagtc ttgtcagtat taacatcttt cttctgagat taatggaggg
     1561 ttgaccgtgt tcagccaact gctgttactc tcgcccagaa atctgcgcca acatggacac
     1621 acccagtgga gcaagccatc tgattttcat ggtcagattg taaagaagca attactcttg
     1681 gacagtttaa gcagaaaagc caccaagtca agtcactggt catatgatga actcgaagat
     1741 ctaataaaag ttaatttaag cgttcattac aataaattca agtgaatctg ttttaagcta
     1801 gcctttaacg tttttaaatt ccttcaaaca aattggatcc tctttgcagt tcctcatttc
     1861 tcggtgagct ggtgctttaa tccctttact ttttcttcct ctagggagtg atggttggca
     1921 tgggacgaat taattcctgc agcccggggg taccggtcgc caccatgaac accccgggaa
     1981 ttaacctgat caaggaggac atgcgcgtga aggtgcacat ggagggcaac gtgaacggcc
     2041 acgccttcgt gatcgagggc gagggcaagg gcaagcccta cgagggcacc cagaccgcca
     2101 acctgaccgt gaaggagggc gcccccctgc ccttcagcta cgacatcctg accaccgccg
     2161 tgcactacgg caaccgggtg ttcaccaagt accccgagga catccccgac tacttcaagc
     2221 agagcttccc cgagggctac agctgggagc gcaccatgac cttcgaggac aagggcatct
     2281 gcaccatccg cagcgacatc agcctggagg gcgactgctt cttccagaac gtgcgcttca
     2341 agggcaccaa cttccccccc aacggccccg tgatgcagaa gaaaaccctg aagtgggagc
     2401 ccagcaccga gaagctgcac gtgcgcgacg gcctgctggt gggcaacatc aacatggccc
     2461 tgctgctgga gggcggcggc cactacctgt gcgacttcaa gaccacctac aaggccaaga
     2521 aggtggtgca gctgcccgac gcccacttcg tggaccaccg catcgagatc ctgggcaacg
     2581 acagcgacta caacaaggtg aagctgtacg agcacgccgt ggcccgctac agccccctgc
     2641 ccagccaggt gtggtaaccg gtccaccacc accaccacca ctaaggatcc ggggttgggg
     2701 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     2761 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     2821 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     2881 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     2941 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     3001 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     3061 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     3121 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     3181 atcaccgacc tctctcccca gcaattcacc atgaccgagt acaagcccac ggtgcgcctc
     3241 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     3301 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     3361 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     3421 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     3481 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     3541 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     3601 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     3661 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     3721 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     3781 tggtgcatga cccgcaagcc cggtgccgaa ggtagaggtt ctctcctcac ttgtggtgat
     3841 gttgaagaaa accctggtcc aatgtctaga ctggacaaga gcaaagtcat aaacggagct
     3901 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     3961 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     4021 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     4081 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     4141 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     4201 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     4261 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     4321 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     4381 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     4441 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     4501 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     4561 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat
     4621 cgatccaaga tatcgtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4681 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4741 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4801 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4861 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4921 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4981 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     5041 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5101 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5161 ctccctttgg gccgcctccc cgcctgtttc gcctcggcgt ccggactaga ggtaccttta
     5221 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5281 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5341 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5401 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5461 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5521 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5581 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5641 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5701 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     5761 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5821 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5881 gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5941 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6001 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6061 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6121 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6181 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6241 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6301 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6361 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6421 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6481 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6541 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6601 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6661 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6721 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6781 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6841 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6901 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7621 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7681 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7741 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7801 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7861 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7921 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7981 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     8041 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     8101 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     8161 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     8221 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     8281 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8341 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8401 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     8461 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     8521 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8581 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8641 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8701 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8761 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8821 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8881 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8941 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9001 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9061 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9121 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9181 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9241 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9301 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     9361 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9421 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     9481 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9541 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     9601 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9661 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     9721 tcagacccac ctcccaaccc cgaggggac
//
