LOCUS       Exported               10457 bp ds-DNA     circular SYN 06-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2018 from SnapGene Viewer 4.2.8
            http://www.snapgene.com
COMMENT     [DEFINITION]: . [LOCUS]: New_DNA [ACCESSION]:
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      17..457
                     /direction=RIGHT
                     /label=F1 ori
     CDS             complement(464..532)
                     /label=LacZ alpha
     primer_bind     603..620
                     /label=M13-fwd
     primer_bind     630..649
                     /label=T7
     misc_recomb     complement(1324..1344)
                     /label=attB4
     primer_bind     3790..3807
                     /label=M13-fwd
     primer_bind     3817..3836
                     /label=T7
     CDS             3900..4604
                     /label=mCherry
     misc_feature    4671..5567
                     /label=CDK2 DN
     misc_feature    4893..4899
                     /label=DN site
     primer_bind     complement(5634..5653)
                     /label=T3
     polyA_signal    5716..5907
                     /label=SV40 late polyA
     primer_bind     complement(8271..8290)
                     /label=T3
     primer_bind     complement(8308..8328)
                     /label=M13-rev
     misc_binding    complement(8334..8356)
                     /label=LacO
     promoter        complement(8361..8390)
                     /label=lac
     rep_origin      complement(8696..9324)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(9476..10135)
                     /label=AmpR
     promoter        complement(10375..10403)
                     /label=Amp prom
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttggctccg aattcgccct taaaactcga gagtttgact
     1381 tgggttgatt atagcaaata gtggaacaca aacagatttt gaaatattct tgcaaattta
     1441 tttattaaat ccattcaaat attgtcattt tttgtactgt gtaatttata ttgatttgct
     1501 catacaatat ttacatttcg cttaaacaat acactattat ttatatttaa aaatagctat
     1561 atataaatca aatgtatagc tcttttttac acaattaatc aaagctaaaa tatttgtcac
     1621 acaaatacag taagccactt cacaatgata tcttcaaaat ctgtgcttga ctgaatcatt
     1681 tcatttattg ttttgtaatt cacattatac cttacgttct ccaggcacag tttgaattat
     1741 tgataaatga agataataca tctgatttaa ttagtctcct ttgtttataa taactttaat
     1801 ctaatttgat agtaataatt ccagcaatac atccattaca ataaaaatcg ctttttaatt
     1861 ttgcacatta gtttgccggt atttgtttaa agtttcacca tacttttaaa tcatgtgcta
     1921 tttgaaaagg gctgttatga ctggaattaa atgttttttt ttttatatca gtgttttaga
     1981 ttgaagtaca atatttatag gtaaaattca ccagcatatg cttaaagata gccatgctcc
     2041 aggtccttgt gtccttctaa ggtctgtttg tggtttaagc tgtttgttga cacacgcttg
     2101 tcttctccat ttttctgtgc agttattgtc agtttattgc acactgggtt gcattaagct
     2161 gcagtttttt cacaacagtt ttccacattg taacagtcag tctccaaatt attccatcag
     2221 tcctcaaatt tagtcatttg aggccatgaa aacacttaaa actacataaa ggtagtttgg
     2281 cattaggttt tagacttgtt cttgtttggc aatattgcaa cctgtgtaaa ataatttgat
     2341 tctttttttt tttttttttt ttttaatttt cagaaagaaa tggtccaaaa ggagctatgt
     2401 gaacatttat ccatgtgttt aatgtttttt tttttattat taaaccactt ctgacttaac
     2461 tttttttagt tattctattt tgtgacgagt ctgaatggcc aaagtacttt tgaatccaaa
     2521 atcttactag taaaatgtta aatcttacta gcagtgttgg gtataatttg taacaaagtt
     2581 aaattccata tctgctaagt gaaaaagtaa agtaagagat tgctgtaaat atttaggtaa
     2641 tttgttttat taatacaata tagaaagtaa caaaacacaa tgtaaaaaaa tcaataaaga
     2701 aagtaaataa taaaagtaat gataaactaa tgatctaaac aaaattagta ataaaattta
     2761 tacaattcag ggagggtaaa gaatttcacc taatagtcaa actctgtatt aaataatata
     2821 aaatatttgt tcttagtctg aagaggagac tgtgagagaa agcttactgg ttattaaatt
     2881 agaaaatttg taacttgcag attaaagctg tgtgccttgc agattaaaat aatttaaata
     2941 atttaataaa ggccatgttt gagacaaatg taaaaacata ataaatgtta agaatttcag
     3001 atattgagat tttacaattt aaaaaaagca ataaagtaat agcagagacc tacactctta
     3061 aagtaaatta ctcaacactg cttactagta acttttggaa tagttactag tatccattga
     3121 acaaatacta gaatctaatt aaattgtgtg agtgtccaat gaaaagattc ttgtatttaa
     3181 aacagtacca gtatctatat atgtacaaaa tttagtgcta gcaaaatgca aataaatact
     3241 actgcatata atggattgca ttgtaaatcg acttgccata atacatttat ttcctatttt
     3301 tacttttaac aaaaaataag ttcactgagt atttcatgtc attaaatcgc attaaacctc
     3361 ttgcatgctt tatgtacact agtgcagttc gctaaatgac caccggtgtc actaataaca
     3421 cgatcgtttc tgaattctgc attaaacgcc atttatacgt tgctcttatt agtaaacaat
     3481 ttctccatca tgagctcgac cagtattgca tatttcatcc tcttaaatcc agagttgttt
     3541 cttttaatca ctttctcttc atgataaaag cagtttttgg ttttcagaag tttacaacaa
     3601 acccagtcag ttattgcctc agcaaacact ggagcaactg cacacttgtg aaaagcttct
     3661 tgcgacacaa acctttctct ggacttataa ataaaggcat tcagcagctc caccatacac
     3721 ctccactggt cttgataaag ggatccaaaa agggcgaatt cgacccaagt ttgtacaaaa
     3781 aagcaggctt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     3841 ttgggtaccg ggccccccct cgaggtcgac tcagcagtga tacaggtacc gccaccatgg
     3901 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     3961 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4021 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     4081 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     4141 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     4201 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     4261 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     4321 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     4381 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     4441 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     4501 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     4561 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggatct ggagccacga
     4621 acttctctct gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct atggagtctt
     4681 ttcagaaagt ggagaagatc ggagagggaa catacggagt ggtttataaa gccaaaaata
     4741 aagttacagg agaaactgtc gcgctgaaga aaatccgatt agatacggaa actgaaggtg
     4801 ttcccagcac tgccattcgt gagatctctc tgctaaaaga gctcaatcac cctaacatag
     4861 tcaagttgcg tgatgtgata cacactgaaa ataaacttta cttggtgttt gagtttcttc
     4921 accaagacct gaagaggttt atggactcga cgtctgtcag tggcatatcc ttgccactcg
     4981 tgaagagtta cctattccag ctgctccagg gattggcctt ctgtcactcc catcgggttc
     5041 ttcacagaga tcttaaaccc cagaatctcc tcatcaacgc tcagggcgag atcaaactgg
     5101 ctaactttgg tttggccaga gcgttcggtg tacctgtgcg gacttataca catgaggttg
     5161 taactttgtg gtacagagct ccagagattc tcctgggatg taaatattat tctacagctg
     5221 ttgacatctg gagtttgggc tgcatctttg ctgaaatgat tactcggagg gcactgtttc
     5281 ctggagactc tgaaatagat cagctcttcc ggatatttcg aactctgggc actcctgatg
     5341 agtctatatg gcctggagtt acatcaatgc cagactacaa accctccttt cccaaatggg
     5401 ctcgacagga cctgtctaaa gtggtgcccc cccttgatga agatggcaga gaccttctcg
     5461 ggcaaatgtt gacctatgat ccaaacaaga ggatctcggc aaagaacgct cttgttcatc
     5521 ggtttttccg cgacgtcacc atgccagtgc ctcctttacg cctataatca taatcagcca
     5581 tggatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     5641 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gcacccagct ttcttgtaca
     5701 aagttgatgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
     5761 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     5821 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     5881 ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg cattgggccc
     5941 tcgagcatat gagatctgtc ccgatctttc tcttctgtgc tgtcaattta gagtagtttt
     6001 tgaggtaatt tgggtgcatt ctctgtaaaa acaacaaata aatatttata ttgtatgcaa
     6061 taaatgtaac aattacacat acactctctg attacagcat taaagggtta gttcacccaa
     6121 aaatgaaaag atgtcattaa tgactcaccc tcatgtcgtt ccaagcccgt aacacctccg
     6181 ttcatcttcg gaacacagtt taagatattt tagatttagt ccgagagctt tctgtccctc
     6241 cactgaaaat gtatgtacgg tatactgtcc atgtccagaa aggtaataaa aacatcaaag
     6301 tagtccatgt gacatcagtg ggttaggtag aattatttga agcatcgaaa atacattttg
     6361 gtccaaaaat aacaaaacct acgactttat tcagcattgt agcgtcactg cgaagtcgtg
     6421 aacgcggatt gacaacagac ccggaagaga atacaatgcc gaataaagtc gtaggttttg
     6481 ttatttttgg accaaaatgt attcgatgct tcaaaaaatt ctaactaacc cactgatgtc
     6541 acatggacta ctttgatgtt tttattacct ttctggacat ggacagtata ccgtacatac
     6601 attctcaatg gaggcacaga aagctctcgg actaaatcta aaatatctta aactgtgttc
     6661 tgaagatgaa cggaggtctt acgggcttgg aacgacatga gggcgagtca ttaatgacat
     6721 tattttcatt tttgggtgaa ctaacccttt aataaaactg acatttgcag ccaattgcac
     6781 actttataat gttaaatact tgttgatttt agaaatacag tagcatttag tgacaatcaa
     6841 acattaattt tatttctgat tatattgaca aaaacacagg actgtaaggc tgacatgaca
     6901 gggtgaattg atttcattac attagttttg atggtaacac ttttacaata aggttcgtta
     6961 gttaactaca ttagttaaca tgaactaata atgaactgca cgtatacatg ttaatttcaa
     7021 catttactaa tactttatat cttgttaaca ttagttaatg cactgtgaac taacatgaac
     7081 aaacaatgaa catctgtatt tctattaact aacgttaaca aagattaaca gatacagtaa
     7141 caaatgtatt gctcatggtt atttaatgtt agttaataca tgaactaatc aacctcattg
     7201 taaagtgtta ccgttttgac aatacaacat gtttggaaaa aaatttctcg cttggttttc
     7261 ttgtgttttg catgaattca aacacaatga gagcacggaa tgataatgat tattcaaagc
     7321 tgcaataggc gccaaatttc ccgtggacct aacgttacca attacaaatg cattttagca
     7381 ttttattacg tgagccttat attgtcaatc gcatgcatta gtgaaaatag gcctactaca
     7441 catataaaag gcgcgccatc aacgtcaaaa catgcacacc caaaaaaaaa aaaaaaaaaa
     7501 aaaaaaaaaa ctatcagtaa aacaaaatac ttaatgtact tacctcaata tgcttcctta
     7561 ggtttgatgg cgaacttttg aaggccgata tttctttatt aagcgggaga cagaggacac
     7621 atttcatctt gaatgaatct ttatttacac cacttaaaga aaagaattcg cgaagatacg
     7681 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg
     7741 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca
     7801 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa
     7861 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa
     7921 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat
     7981 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     8041 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     8101 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     8161 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     8221 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     8281 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8341 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8401 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8461 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8521 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8581 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8641 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9121 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     9181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9541 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9601 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9661 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9721 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9781 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9841 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9901 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9961 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10021 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10081 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10441 catttccccg aaaagtg
//
