LOCUS       Exported                7147 bp ds-DNA     circular SYN 06-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2018 from SnapGene Viewer 4.2.8
            http://www.snapgene.com
COMMENT     [DEFINITION]: [LOCUS]: Construct_1 [ACCESSION]: pVec1
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    650..669
                     /label=PCDNA seq+
     primer_bind     863..882
                     /label=T7
     misc_feature    901..940
                     /label=199 insert+
     misc_feature    1185..1214
                     /label=intron primer
     misc_feature    1344..1618
                     /label=miR-199
     misc_feature    2011..2709
                     /label=dendra2
     misc_feature    2683..2700
                     /label=dendra-primer
     misc_feature    2687..2725
                     /label=199 insert-
     terminator      2744..2971
                     /label=bGH PA
     misc_feature    3639..3716
                     /label=SV40 ORI
     CDS             3858..4649
                     /label=Kan/neoR
     primer_bind     complement(4989..5009)
                     /label=M13-rev
     misc_binding    complement(5015..5037)
                     /label=LacO
     promoter        complement(5042..5071)
                     /label=lac
     CDS             complement(6154..6813)
                     /label=AmpR
     promoter        complement(7053..7081)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgtac atttaataaa ccatcctaat aacatgttaa tcatattgaa
      961 ccacattaaa ctctcatttg tgcattttac atcttttaaa accgatttat attcgttttt
     1021 agataatgaa gtttattaaa gtgttaattc acagctttat tgcctgtttt gagaatctgc
     1081 ttgcataagt gatttttaaa cttgatctga ttctcgtttg tttcgaagtt cttcagcttt
     1141 gaaagtcaga tcttcattca cagccatgcc atagttttag gatacagtat aagtagacga
     1201 attgggatgc atcgtagatt ttatgctgtg tttgttatgg tttttcagtg gtagagattt
     1261 tcagagtatt attagggtta tagtcagtgt caggtttatt tatcgtcagc gtgatgggca
     1321 aaaaacagct ggcagtacgg gctctctcca cagtcgtgtg cggtcctcag ggcagtgacc
     1381 tacgctccag ccacctcttg cctccccctc gcctgcccag tgttcagact acctgttcat
     1441 catgctgcag ctgaacagta gtccgcacat tggttaggct gggctgggac acacacaccc
     1501 gccaagccgc cgtaagatct ctgccattcg ccggtgaacg aataaagcaa gactttagcc
     1561 ccgcacaata caccgtttca gcatcgacat cgcaatgaac cacatcgctg aattcacaag
     1621 ccaactgctg ttactctcgc ccagaaatct gcgccaacat ggacacaccc agtggagcaa
     1681 gccatctgat tttcatggtc agattgtaaa gaagcaatta ctcttggaca gtttaagcag
     1741 aaaagccacc aagtcaagtc actggtcata tgatgaactc gaagatctaa taaaagttaa
     1801 tttaagcgtt cattacaata aattcaagtg aatctgtttt aagctagcct ttaacgtttt
     1861 taaattcctt caaacaaatt ggatcctctt tgcagttcct catttctcgg tgagctggtg
     1921 ctttaatccc tttacttttt cttcctctag ggagtgatgg ttggcatggg acgaattaat
     1981 tcctgcagcc cgggggtacc ggtcgccacc atgaacaccc cgggaattaa cctgatcaag
     2041 gaggacatgc gcgtgaaggt gcacatggag ggcaacgtga acggccacgc cttcgtgatc
     2101 gagggcgagg gcaagggcaa gccctacgag ggcacccaga ccgccaacct gaccgtgaag
     2161 gagggcgccc ccctgccctt cagctacgac atcctgacca ccgccgtgca ctacggcaac
     2221 cgggtgttca ccaagtaccc cgaggacatc cccgactact tcaagcagag cttccccgag
     2281 ggctacagct gggagcgcac catgaccttc gaggacaagg gcatctgcac catccgcagc
     2341 gacatcagcc tggagggcga ctgcttcttc cagaacgtgc gcttcaaggg caccaacttc
     2401 ccccccaacg gccccgtgat gcagaagaaa accctgaagt gggagcccag caccgagaag
     2461 ctgcacgtgc gcgacggcct gctggtgggc aacatcaaca tggccctgct gctggagggc
     2521 ggcggccact acctgtgcga cttcaagacc acctacaagg ccaagaaggt ggtgcagctg
     2581 cccgacgccc acttcgtgga ccaccgcatc gagatcctgg gcaacgacag cgactacaac
     2641 aaggtgaagc tgtacgagca cgccgtggcc cgctacagcc ccctgcccag ccaggtgtgg
     2701 taatctagat ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     2761 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2821 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2881 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2941 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3001 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3061 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3121 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3181 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3241 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3301 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3361 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3421 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3481 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3541 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3601 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3661 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3721 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3781 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     3841 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3901 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3961 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4021 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4081 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4141 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4201 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4261 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4321 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4381 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4441 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4501 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4561 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4621 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     4681 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     4741 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4801 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4861 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4921 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     4981 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5041 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5101 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5821 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     5941 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6001 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6061 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6121 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6181 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6241 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6301 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6361 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6421 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6481 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6541 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6601 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6661 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6721 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6781 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6841 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6901 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6961 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7021 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7081 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7141 tgacgtc
//
