LOCUS       Exported                6896 bp ds-DNA     circular SYN 06-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2018 from SnapGene Viewer 4.2.8
            http://www.snapgene.com
COMMENT     [DEFINITION]: [LOCUS]: Construct_1 [ACCESSION]: pVec1
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     863..882
                     /label=T7
     misc_feature    917..1699
                     /label=intron insert
     misc_feature    1185..1214
                     /label=intron
     misc_feature    1760..2458
                     /label=dendra2
     misc_feature    2432..2449
                     /label=dendra primer
     terminator      2493..2720
                     /label=bGH PA
     misc_feature    3388..3465
                     /label=SV40 ORI
     CDS             3607..4398
                     /label=Kan/neoR
     primer_bind     complement(4738..4758)
                     /label=M13-rev
     misc_binding    complement(4764..4786)
                     /label=LacO
     promoter        complement(4791..4820)
                     /label=lac
     CDS             complement(5903..6562)
                     /label=AmpR
     promoter        complement(6802..6830)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgtac atttaataaa ccatcctaat aacatgttaa tcatattgaa
      961 ccacattaaa ctctcatttg tgcattttac atcttttaaa accgatttat attcgttttt
     1021 agataatgaa gtttattaaa gtgttaattc acagctttat tgcctgtttt gagaatctgc
     1081 ttgcataagt gatttttaaa cttgatctga ttctcgtttg tttcgaagtt cttcagcttt
     1141 gaaagtcaga tcttcattca cagccatgcc atagttttag gatacagtat aagtagacga
     1201 attgggatgc atcgtagatt ttatgctgtg tttgttatgg tttttcagtg gtagagattt
     1261 tcagagtatt attagggtta tagtcagtgt caggtttatt tatcgtcagc gtgatgggca
     1321 aaaaacagct ggcagtacgg gctatgtctt cgtctagaag aagacaaagc caactgctgt
     1381 tactctcgcc cagaaatctg cgccaacatg gacacaccca gtggagcaag ccatctgatt
     1441 ttcatggtca gattgtaaag aagcaattac tcttggacag tttaagcaga aaagccacca
     1501 agtcaagtca ctggtcatat gatgaactcg aagatctaat aaaagttaat ttaagcgttc
     1561 attacaataa attcaagtga atctgtttta agctagcctt taacgttttt aaattccttc
     1621 aaacaaattg gatcctcttt gcagttcctc atttctcggt gagctggtgc tttaatccct
     1681 ttactttttc ttcctctagg gagtgatggt tggcatggga cgaattaatt cctgcagccc
     1741 gggggtaccg gtcgccacca tgaacacccc gggaattaac ctgatcaagg aggacatgcg
     1801 cgtgaaggtg cacatggagg gcaacgtgaa cggccacgcc ttcgtgatcg agggcgaggg
     1861 caagggcaag ccctacgagg gcacccagac cgccaacctg accgtgaagg agggcgcccc
     1921 cctgcccttc agctacgaca tcctgaccac cgccgtgcac tacggcaacc gggtgttcac
     1981 caagtacccc gaggacatcc ccgactactt caagcagagc ttccccgagg gctacagctg
     2041 ggagcgcacc atgaccttcg aggacaaggg catctgcacc atccgcagcg acatcagcct
     2101 ggagggcgac tgcttcttcc agaacgtgcg cttcaagggc accaacttcc cccccaacgg
     2161 ccccgtgatg cagaagaaaa ccctgaagtg ggagcccagc accgagaagc tgcacgtgcg
     2221 cgacggcctg ctggtgggca acatcaacat ggccctgctg ctggagggcg gcggccacta
     2281 cctgtgcgac ttcaagacca cctacaaggc caagaaggtg gtgcagctgc ccgacgccca
     2341 cttcgtggac caccgcatcg agatcctggg caacgacagc gactacaaca aggtgaagct
     2401 gtacgagcac gccgtggccc gctacagccc cctgcccagc caggtgtggt aatctagatc
     2461 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     2521 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2581 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     2641 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     2701 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     2761 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     2821 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     2881 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     2941 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3001 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3061 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3121 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3181 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3241 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3301 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3361 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3421 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     3481 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3541 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     3601 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     3661 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3721 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     3781 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     3841 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     3901 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     3961 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     4021 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     4081 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     4141 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     4201 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4261 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     4321 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     4381 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     4441 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4501 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4561 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4621 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4681 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     4741 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4801 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4861 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4921 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
     5701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
