LOCUS       pcDNA-SP-GFP-PDL1\CDS        6700 bp    DNA     circular     25-OCT-2017
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11998702|
COMMENT     VNTDBDATE|-11998702|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA-SP-GFP-PDL1 CDS|
COMMENT     VNTAUTHORNAME|mskcc|
COMMENT     VNTOAUTHORNAME|Christine_Mayr|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pcDNA-SP-GFP-PDL1 CDS" 0 6700 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaI" "ApaLI" "AvaI" "BamHI" "BbsI" "BglII" "BsrGI" "ClaI" 
COMMENT         "EcoRI" "HindIII" "KpnI" "MluI" "NcoI" "NheI" "NotI" "PstI" "PvuI" 
COMMENT         "PvuII" "SacI" "SacII" "SalI" "SmaI" "SpeI" "SphI" "StuI" "XbaI" "XhoI" 
COMMENT         "XmaI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 1 0 0 0 0 1 6700 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 1879 0 2523 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 33 0 5202 0 6445 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 2507 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2056 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 1845 0 2739 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BglII" "agatct" 2 0 1 13 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1671 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2189 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 229 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 611 0 3483 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 896 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2500 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "PstI" "ctgcag" 6 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 6148 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 2288 0 2796 0 4712 0 0 
COMMENT             "") #18=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 819 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 3818 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 4514 0 6699 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 250 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 2750 0 3324 0 3396 0 0 
COMMENT             "") 
COMMENT        #24=(CRSite (CStringList) "StuI" "aggcct" 4 0 2 3575 0 3935 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2513 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2507 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #28=(CFSignal (CObList) "EGFP" 21 0 0 961 1677 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "961..1677") 
COMMENT        #29=(CFSignal (CObList) "polyA" 21 0 0 2546 2773 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5245..5472") 
COMMENT        #30=(CFSignal (CObList) "Signal peptide" 21 0 0 907 960 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "907..960") 
COMMENT        #31=(CFSignal (CObList) "PDL1 CDS" 4 0 0 1678 2496 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1678..2496")) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA 'pcDNA-SP-GFP-PDL1 CDS'" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Christine_Mayr" 
COMMENT                          1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 10/25/17 09:37AM" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/25/17 09:37AM" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6700 bp" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 9-OCT-2017" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 1678  End: 2496" 1) 
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COMMENT                                        "   1678..2496" 1))) "PDL1 CDS"))) 
COMMENT                          "CDS (1 total)") 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   907..960" 1))) "Signal peptide") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 961   End: 1677" 1) 
COMMENT                                   #62=(CLinePar 
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COMMENT                                        "   961..1677" 1))) "EGFP") 
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COMMENT                                        "Start: 2546  End: 2773" 1) 
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COMMENT                                        " N2: 2523 " 1))))) "ApaI: 2 sites") 5 1 
COMMENT                          "GGGCCC" "CCCGGG") 
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COMMENT                                        " N1: 33 " 1) 
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COMMENT                          5 "GTGCAC" "CACGTG") 
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COMMENT                          "CYCGRG" "GRGCYC") 
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COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                          12 "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                          "AGATCT" "TCTAGA") 
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COMMENT                          "TGTACA" "ACATGT") 
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COMMENT                          5 "AAGCTT" "TTCGAA") 
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COMMENT                            (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" 
COMMENT                          "CCATGG") 
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COMMENT                           5 "GCTAGC" "CGATCG") 
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COMMENT                                           " N1: 2500 " 1))))) "NotI: 1 site") 2 
COMMENT                           6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                             (CObjectList)) "PstI: 0 sites") 5 1 "CTGCAG" 
COMMENT                           "GACGTC") 
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COMMENT                           2 "CGATCG" "GCTAGC") 
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COMMENT                           1 "GAGCTC" "CTCGAG") 
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COMMENT                           1 5 "GTCGAC" "CAGCTG") 
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COMMENT                           5 "ACTAGT" "TGATCA") 
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FEATURES             Location/Qualifiers
     misc_feature    961..1677
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    2546..2773
                     /vntifkey="21"
                     /label=polyA
     misc_feature    907..960
                     /vntifkey="21"
                     /label=Signal\peptide
     CDS             1678..2496
                     /vntifkey="4"
                     /label=PDL1\CDS
BASE COUNT     1615 a      1821 c      1712 g      1552 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gccaccatga ggatatttgc tgtctttata ttcatgacct actggcattt gctgaacgca 
      961 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 
     1021 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 
     1081 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 
     1141 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 
     1201 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 
     1261 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 
     1321 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 
     1381 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 
     1441 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 
     1501 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 
     1561 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 
     1621 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagttt 
     1681 actgtcacgg ttcccaagga cctatatgtg gtagagtatg gtagcaatat gacaattgaa 
     1741 tgcaaattcc cagtagaaaa acaattagac ctggctgcac taattgtcta ttgggaaatg 
     1801 gaggataaga acattattca atttgtgcat ggagaggaag acctgaaggt tcagcatagt 
     1861 agctacagac agagggcccg gctgttgaag gaccagctct ccctgggaaa tgctgcactt 
     1921 cagatcacag atgtgaaatt gcaggatgca ggggtgtacc gctgcatgat cagctatggt 
     1981 ggtgccgact acaagcgaat tactgtgaaa gtcaatgccc catacaacaa aatcaaccaa 
     2041 agaattttgg ttgtggatcc agtcacctct gaacatgaac tgacatgtca ggctgagggc 
     2101 taccccaagg ccgaagtcat ctggacaagc agtgaccatc aagtcctgag tggtaagacc 
     2161 accaccacca attccaagag agaggagaag cttttcaatg tgaccagcac actgagaatc 
     2221 aacacaacaa ctaatgagat tttctactgc acttttagga gattagatcc tgaggaaaac 
     2281 catacagctg aattggtcat cccagaacta cctctggcac atcctccaaa tgaaaggact 
     2341 cacttggtaa ttctgggagc catcttatta tgccttggtg tagcactgac attcatcttc 
     2401 cgtttaagaa aagggagaat gatggatgtg aaaaaatgtg gcatccaaga tacaaactca 
     2461 aagaagcaaa gtgatacaca tttggaggag acgtaaggcg gccgcctcga gtctagaggg 
     2521 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 
     2581 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 
     2641 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 
     2701 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 
     2761 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 
     2821 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 
     2881 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 
     2941 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 
     3001 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 
     3061 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 
     3121 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 
     3181 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 
     3241 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 
     3301 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     3361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     3421 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     3481 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
     3541 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccaa 
     3601 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 
     3661 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 
     3721 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 
     3781 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 
     3841 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 
     3901 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 
     3961 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 
     4021 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 
     4081 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 
     4141 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 
     4201 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 
     4261 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 
     4321 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 
     4381 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     4441 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     4501 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 
     4561 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     4621 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     4681 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     4741 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     4801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     4861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     4921 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     4981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     5041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     5101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     5161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     5221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     5281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     5341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 
     5401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     5461 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     5521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     5581 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     5641 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     5701 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     5761 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     5821 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     5881 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     5941 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     6001 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     6061 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     6121 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     6181 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     6241 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     6301 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     6361 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     6421 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     6481 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     6541 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     6601 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     6661 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
//
