LOCUS       pcDNA-puro-mCherry-ZFP36L1\isoform1\CDS        6850 bp    DNA     circular      8-MAR-2017
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11593324|
COMMENT     VNTDBDATE|-11666284|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA-puro-mCherry-ZFP36L1 isoform1 CDS|
COMMENT     VNTAUTHORNAME|mskcc|
COMMENT     VNTOAUTHORNAME|Christine_Mayr|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pcDNA-puro-mCherry-ZFP36L1 isoform1 CDS" 0 6850 
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COMMENT         "EcoRI" "HindIII" "KpnI" "MluI" "NcoI" "NheI" "NotI" "PvuI" "PvuII" 
COMMENT         "SacI" "SacII" "SalI" "SmaI" "SpeI" "SphI" "StuI" "XbaI" "XhoI") 
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COMMENT            0 "") 
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COMMENT            0 2411 0 2657 0 0 "") 
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COMMENT        #6=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1602 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2624 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 229 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 611 0 1335 0 3633 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 896 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2651 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 6298 0 0 "") 
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COMMENT        #19=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 4664 0 6849 0 0 "") 
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COMMENT        #24=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2663 0 0 "") 
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COMMENT        #28=(CFSignal (CObList) "CMV immearly promoter" 30 0 0 236 852 0 
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COMMENT        #29=(CFSignal (CObList) "ZFP36L1 isoform1 CDS" 4 0 0 1609 2622 0 
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COMMENT                          "Currently local object. Original author: Christine_Mayr" 
COMMENT                          1) 
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COMMENT                          "Created: 01/16/17 08:19PM" 1) 
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COMMENT                          "Last Modified: 03/08/17 12:19PM" 1) 
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FEATURES             Location/Qualifiers
     misc_feature    904..1608
                     /vntifkey="21"
                     /label=mCherry
     promoter        863..881
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV\immearly\promoter
     CDS             1609..2622
                     /vntifkey="4"
                     /label=ZFP36L1\isoform1\CDS
BASE COUNT     1519 a      2013 c      1789 g      1529 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag 
      961 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 
     1021 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc 
     1081 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac 
     1141 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 
     1201 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac 
     1261 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta 
     1321 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 
     1381 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct 
     1441 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc 
     1501 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa 
     1561 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagac caccaccctc 
     1621 gtgtctgcca ccatcttcga cttgagcgaa gttttatgca agggtaacaa gatgctcaac 
     1681 tatagtgctc ccagtgcagg gggttgcctg ctggacagaa aggcagtggg cacccctgct 
     1741 ggtgggggct tccctcggag gcactcagtc accctgccca gctccaagtt ccaccagaac 
     1801 cagctcctca gcagcctcaa gggtgagcca gcccccgctc tgagctcgcg agacagccgc 
     1861 ttccgagacc gctccttctc ggaagggggc gagcggctgc tgcccaccca gaagcagccc 
     1921 gggggcggcc aggtcaactc cagccgctac aagacggagc tgtgccgccc ctttgaggaa 
     1981 aacggtgcct gtaagtacgg ggacaagtgc cagttcgcac acggcatcca cgagctccgc 
     2041 agcctgaccc gccaccccaa gtacaagacg gagctgtgcc gcaccttcca caccatcggc 
     2101 ttttgcccct acgggccccg ctgccacttc atccacaacg ctgaagagcg ccgtgccctg 
     2161 gccggggccc gggacctctc cgctgaccgt ccccgcctcc agcatagctt tagctttgct 
     2221 gggtttccca gtgccgctgc caccgccgct gccaccgggc tgctggacag ccccacgtcc 
     2281 atcaccccac cccctattct gagcgccgat gacctcctgg gctcacctac cctgcccgat 
     2341 ggcaccaata acccttttgc cttctccagc caggagctgg caagcctctt tgcccctagc 
     2401 atggggctgc ccgggggtgg ctccccgacc accttcctct tccggcccat gtccgagtcc 
     2461 cctcacatgt ttgactctcc ccccagccct caggattctc tctcggacca ggagggctac 
     2521 ctgagcagct ccagcagcag ccacagtggc tcagactccc cgaccttgga caactcaaga 
     2581 cgcctgccca tcttcagcag actttccatc tcagatgact aagaattctg cagatatcca 
     2641 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact 
     2701 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 
     2761 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 
     2821 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 
     2881 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 
     2941 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 
     3001 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 
     3061 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 
     3121 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 
     3181 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 
     3241 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 
     3301 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 
     3361 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 
     3421 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
     3481 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
     3541 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
     3601 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     3661 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     3721 gaggcctagg cttttgcaaa aagctcccaa ccatgaccga gtacaagccc acggtgcgcc 
     3781 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 
     3841 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 
     3901 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 
     3961 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 
     4021 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 
     4081 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 
     4141 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 
     4201 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 
     4261 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 
     4321 cctggtgcat gacccgcaag cccggtgcct gagcgggact ctggggttcg aaatgaccga 
     4381 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 
     4441 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 
     4501 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata 
     4561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     4621 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag 
     4681 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     4741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 
     4801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     4861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     4921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     4981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     5041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     5101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     5161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     5221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     5281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     5341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     5401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     5461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     5521 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     5581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt 
     5641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     5701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     5761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     5821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     5881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     5941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     6001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     6061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     6121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     6181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     6241 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     6301 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     6361 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     6421 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     6481 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     6541 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     6601 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     6661 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     6721 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     6781 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     6841 acctgacgtc 
//
