LOCUS       CAG_MCS_T2A_EGFP       12475 bp ds-DNA     circular     02-NOV-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    8250..8251
                     /locus_tag="EGFP"
                     /label="EGFP"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2193..7459
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct"
                     /label="source:synthetic DNA construct"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7172..7420
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                     subtilis bacteriophage (Mol et al., 1995)"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     A YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
                     /locus_tag="UGI"
                     /label="UGI"
                     /ApEinfo_label="UGI"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(10549..11231)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    9562..9773
                     /locus_tag="bGH pA"
                     /label="bGH pA"
                     /ApEinfo_label="bGH pA"
                     /ApEinfo_fwdcolor="#ffcccc"
                     /ApEinfo_revcolor="#ffcccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_site      9993..10323
                     /locus_tag="SV40ori/pA'"
                     /label="SV40ori/pA'"
                     /ApEinfo_label="SV40ori/pA'"
                     /ApEinfo_fwdcolor="#ffd39f"
                     /ApEinfo_revcolor="#ffd39f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7466..7528
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             8863..9462
                     /locus_tag="PuroR"
                     /label="PuroR"
                     /ApEinfo_label="PuroR"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7535..8249
                     /locus_tag="EGFP(1)"
                     /label="EGFP(1)"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8287..8859
                     /locus_tag="IRES"
                     /label="IRES"
                     /ApEinfo_label="IRES"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     regulatory      2198..2207
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /locus_tag="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /label="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /ApEinfo_label="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7433..7453
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(11329..11988)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2207..2272
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /locus_tag="3xFLAG"
                     /label="3xFLAG"
                     /ApEinfo_label="3xFLAG"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1..185)
                     /locus_tag="Polyoma Ori'"
                     /label="Polyoma Ori'"
                     /ApEinfo_label="Polyoma Ori'"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2279..2299
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /locus_tag="SV40 NLS(1)"
                     /label="SV40 NLS(1)"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        838..2079
                     /locus_tag="CAG pro"
                     /label="CAG pro"
                     /ApEinfo_label="CAG pro"
                     /ApEinfo_fwdcolor="#0066ff"
                     /ApEinfo_revcolor="#0066ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2324..3010
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from
                     rat"
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINW
                     G
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEF
                     L
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPS
                     N
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPH
                     I LWATGLK"
                     /locus_tag="APOBEC-1"
                     /label="APOBEC-1"
                     /ApEinfo_label="APOBEC-1"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     enhancer        468..826
                     /locus_tag="HCMVIEE"
                     /label="HCMVIEE"
                     /ApEinfo_label="HCMVIEE"
                     /ApEinfo_fwdcolor="#996633"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3059..7156
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     N
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     F
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     F
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     E
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQ
                     I
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVR
                     Q
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     R
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     N
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     F
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     S
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     L
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFI
                     Q
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     H
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     L
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     S
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     V
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     N
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     E
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     E
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     T
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNE
                     L
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     A
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     D ATLIHQSITGLYETRIDLSQLGG"
                     /locus_tag="Cas9"
                     /label="Cas9"
                     /ApEinfo_label="Cas9"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TTGTCATAGT AATGATTGGG TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC
       61 CTCTTGCAAA ACCACACTGA CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG
      121 CAAAACCACA CTGCCCTCTG GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC
      181 ACGGCCAGCT GCGGTCAGTT AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA
      241 AGAGTCGGGA GGAAAATTAC TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA
      301 ATTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
      361 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
      421 TCTTATCATG TCTGGATCTG ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG
      481 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
      541 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
      601 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
      661 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
      721 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
      781 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG
      841 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT
      901 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG GGGGGGGGGG GGGGCGCGCG
      961 CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG GGCGGGGCGA GGCGGAGAGG TGCGGCGGCA
     1021 GCCAATCAGA GCGGCGCGCT CCGAAAGTTT CCTTTTATGG CGAGGCGGCG GCGGCGGCGG
     1081 CCCTATAAAA AGCGAAGCGC GCGGCGGGCG GGAGTCGCTG CGCGCTGCCT TCGCCCCGTG
     1141 CCCCGCTCCG CCGCCGCCTC GCGCCGCCCG CCCCGGCTCT GACTGACCGC GTTACTCCCA
     1201 CAGGTGAGCG GGCGGGACGG CCCTTCTCCT CCGGGCTGTA ATTAGCGCTT GGTTTAATGA
     1261 CGGCTTGTTT CTTTTCTGTG GCTGCGTGAA AGCCTTGAGG GGCTCCGGGA GGGCCCTTTG
     1321 TGCGGGGGGA GCGGCTCGGG GGGTGCGTGC GTGTGTGTGT GCGTGGGGAG CGCCGCGTGC
     1381 GGCTCCGCGC TGCCCGGCGG CTGTGAGCGC TGCGGGCGCG GCGCGGGGCT TTGTGCGCTC
     1441 CGCAGTGTGC GCGAGGGGAG CGCGGCCGGG GGCGGTGCCC CGCGGTGCGG GGGGGGCTGC
     1501 GAGGGGAACA AAGGCTGCGT GCGGGGTGTG TGCGTGGGGG GGTGAGCAGG GGGTGTGGGC
     1561 GCGTCGGTCG GGCTGCAACC CCCCCTGCAC CCCCCTCCCC GAGTTGCTGA GCACGGCCCG
     1621 GCTTCGGGTG CGGGGCTCCG TACGGGGCGT GGCGCGGGGC TCGCCGTGCC GGGCGGGGGG
     1681 TGGCGGCAGG TGGGGGTGCC GGGCGGGGCG GGGCCGCCTC GGGCCGGGGA GGGCTCGGGG
     1741 GAGGGGCGCG GCGGCCCCCG GAGCGCCGGC GGCTGTCGAG GCGCGGCGAG CCGCAGCCAT
     1801 TGCCTTTTAT GGTAATCGTG CGAGAGGGCG CAGGGACTTC CTTTGTCCCA AATCTGTGCG
     1861 GAGCCGAAAT CTGGGAGGCG CCGCCGCACC CCCTCTAGCG GGCGCGGGGC GAAGCGGTGC
     1921 GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC
     1981 CTTCTCCCTC TCCAGCCTCG GGGCTGTCCG CGGGGGGACG GCTGCCTTCG GGGGGGACGG
     2041 GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT CTAGAGCCTC TGCTAACCAT
     2101 GTTCATGCCT TCTTCTTTTT CNTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT
     2161 CATCATTTTG GCAAAGAATT CCTCGaccgg tAgatccgcc accatggact ataaggacca
     2221 cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc
     2281 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccatgagct cagagactgg
     2341 cccagtggct gtggacccca cattgagacg gcggatcgag ccccatgagt ttgaggtatt
     2401 cttcgatccg agagagctcc gcaaggagac ctgcctgctt tacgaaatta attggggggg
     2461 ccggcactcc atttggcgac atacatcaca gaacactaac aagcacgtcg aagtcaactt
     2521 catcgagaag ttcacgacag aaagatattt ctgtccgaac acaaggtgca gcattacctg
     2581 gtttctcagc tggagcccat gcggcgaatg tagtagggcc atcactgaat tcctgtcaag
     2641 gtatccccac gtcactctgt ttatttacat cgcaaggctg taccaccacg ctgacccccg
     2701 caatcgacaa ggcctgcggg atttgatctc ttcaggtgtg actatccaaa ttatgactga
     2761 gcaggagtca ggatactgct ggagaaactt tgtgaattat agcccgagta atgaagccca
     2821 ctggcctagg tatccccatc tgtgggtacg actgtacgtt cttgaactgt actgcatcat
     2881 actgggcctg cctccttgtc tcaacattct gagaaggaag cagccacagc tgacattctt
     2941 taccatcgct cttcagtctt gtcattacca gcgactgccc ccacacattc tctgggccac
     3001 cgggttgaaa agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga
     3061 caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac
     3121 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
     3181 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
     3241 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
     3301 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
     3361 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
     3421 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
     3481 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
     3541 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
     3601 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
     3661 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
     3721 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat
     3781 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatac
     3841 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
     3901 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct
     3961 gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat
     4021 caagctgtac gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca
     4081 gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta
     4141 cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa
     4201 gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca
     4261 gcggaccttc gacaacggca tcatccccca ccagatccac ctgggagagc tgcacgccat
     4321 tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa
     4381 gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt
     4441 cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg agaaggtggt
     4501 ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct
     4561 gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa
     4621 cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg
     4681 cgaccagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa
     4741 gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg
     4801 cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa
     4861 ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac
     4921 cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct
     4981 gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct
     5041 gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt
     5101 cctgaagtcc gacggcttcg ccaacagaaa cttcatccag ctgatccacg acgacagcct
     5161 gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga
     5221 gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa
     5281 ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga
     5341 aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa
     5401 gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga
     5461 aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat
     5521 gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt
     5581 gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa
     5641 gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta
     5701 ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa
     5761 ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt
     5821 ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa
     5881 gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct
     5941 ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca
     6001 ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc
     6061 taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat
     6121 cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
     6181 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     6241 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     6301 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     6361 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
     6421 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
     6481 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     6541 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     6601 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     6661 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg tgctgcagaa
     6721 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     6781 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
     6841 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
     6901 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
     6961 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
     7021 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
     7081 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
     7141 gtctcagctg ggaggcgatt caggcggatc tactaatctg tcagatatta ttgaaaagga
     7201 gaccggtaag caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga
     7261 agtcattggg aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac
     7321 cgacgagaat gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt
     7381 catacaggat agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa
     7441 gaagaggaaa gtcgctagcG TCGACggctc cggagagggc agaggaagtc tgctaacatg
     7501 cggtgacgtc gaggagaatc ctggcccaAT CGATatggtg agcaagggcg aggagctgtt
     7561 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     7621 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcAtctg
     7681 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     7741 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     7801 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     7861 ccgcgccgag gtgaagttcg agggcgacac cctggtGaac cgcatcgagc tgaagggcat
     7921 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     7981 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     8041 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     8101 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     8161 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     8221 gatcactctc ggcatggacg agctgtacaa gtaaTTTAAA GAATTCCGCC CCCCCCCCCT
     8281 CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT
     8341 TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC
     8401 CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC AAAGGAATGC
     8461 AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA AGACAAACAA
     8521 CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG TGCCTCTGCG
     8581 GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG TGCCACGTTG
     8641 TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC AACAAGGGGC
     8701 TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT CGGTGCACAT
     8761 GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
     8821 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA GTACAAGCCC
     8881 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG
     8941 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC
     9001 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC
     9061 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG
     9121 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG
     9181 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
     9241 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC
     9301 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC
     9361 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA
     9421 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG
     9481 CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG CTCGCTGATC
     9541 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC
     9601 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC
     9661 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG
     9721 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA
     9781 GGCGGAAAGA ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
     9841 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
     9901 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
     9961 CCAGTCGGGA AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC
    10021 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
    10081 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA
    10141 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT
    10201 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG
    10261 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG
    10321 TCTGGATCCG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
    10381 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
    10441 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
    10501 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
    10561 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
    10621 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
    10681 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
    10741 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
    10801 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
    10861 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
    10921 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
    10981 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
    11041 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
    11101 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
    11161 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
    11221 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
    11281 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
    11341 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
    11401 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
    11461 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
    11521 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
    11581 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
    11641 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
    11701 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
    11761 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
    11821 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
    11881 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
    11941 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
    12001 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
    12061 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
    12121 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
    12181 ACTCATACTC TTCCTTTTTC AATGACCGCT TTGGAAAAAC AAAGACTGTA TTTCCTGGAA
    12241 ATTAATGTTT ATTCAATAAA CTGTGTATTC AGCTATATTC ACATAGTGGT GAGGCTGAAA
    12301 TGAGGCGGGA AGAGGCGGTT GGGGCTTAAT TATATCAATT TGGGTGGCCC CACAGCGCCT
    12361 CCAAGGCGCC AGTCCTGTTT TGACAAGTTG CCTCTGGAAG CCTCTCTTCT TTTTCTCCAG
    12421 AGTAAGCGGA GGCCAGGGGC CCCCGGCCTC TGCTTAATAC TAAAAAAAAC AGCTG
//
